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  本文關(guān)鍵詞：食用白酒對(duì)大鼠心臟ACE、ACE2表達(dá)的影響　出處：《遵義醫(yī)學(xué)院》2012年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的：在一定時(shí)間內(nèi)連續(xù)給大鼠灌胃不同劑量的食用白酒,觀察心臟結(jié)構(gòu)、功能及檢測心肌組織中ACE、ACE2及AngⅡ的變化。 方法： SD大鼠124只按4wk、8wk、12wk三個(gè)時(shí)間點(diǎn)隨機(jī)分成白酒組、酒精組、生理鹽水組,每組分別又分為低、中和高劑量組,每組6只,共27組。按低劑量(0.25ml/100g/d)、中劑量(0.5ml/100g/d)、高劑量(1ml/100g/d),每日量分兩次(每次按半量)給予大鼠灌胃,兩次間隔時(shí)間超過9h以上。每周稱體重一次,根據(jù)體重調(diào)整一周的灌胃量。分別在上述三個(gè)時(shí)間點(diǎn)測定左心室內(nèi)壓變化幅度(LVP),計(jì)算心臟／體重指數(shù)(HW/BW), Masson染色觀察心肌纖維化,ELISA檢測心肌組織AAngⅡ、ACE、ACE2含量,RT-PCR檢測心肌細(xì)胞ACEmRNA和ACE2mRNA的表達(dá)。 結(jié)果一：①4wk末白酒組的各劑量組左心室內(nèi)壓變化幅度、HW/BW與酒精組、生理鹽水組比較無顯著性差異(P0.05)。②4wk末白酒組、酒精組的中、高劑量組的心肌細(xì)胞胞漿輕度渾濁、變性,纖維組織輕度增生,且以高劑量變化明顯。③4wk末白酒組、酒精組的各劑量組的心肌組織AngⅡ、ACE、ACE2含量與生理鹽水組比較,無顯著性差異(P0.05),但出現(xiàn)隨劑量增加而升高的趨勢(shì)。④4wk末白酒組、酒精組的各劑量組心肌組織ACEmRNA、ACE2mRNA的表達(dá)與生理鹽水組比較,無明顯變化(P0.05)。 結(jié)果二：①8wk末白酒組的各劑量組LVP、HW/BW與酒精組、生理鹽水組比較無顯著性差異(P0.05)。②在8wk末,隨著時(shí)間延長,從低劑量組到高劑量組的心肌細(xì)胞胞漿逐漸發(fā)生變性、渾濁,纖維組織逐漸增生明顯,各劑量組中以酒精組變化明顯。③8wk末白酒的中、高劑量組和酒精的中、高劑量組心肌組織AngⅡ、ACE含量分別高于生理鹽水對(duì)照組、低劑量組(P0.05,P0.01),且酒精的高劑量組心肌組織ACE含量高于中劑量組(P0.05)。④8wk末白酒和酒精的低、中、高劑量組心肌組織ACE2含量均高于生理鹽水對(duì)照組(P0.05,P0.01,P0.01),白酒和酒精的中劑量組、高劑量組和心肌組織ACE2含量分別高于各自的低劑量組(P0.05,P0.01),且高劑量組心肌組織ACE2還含量高于中劑量組(P0.05)。⑤8wk末白酒和酒精中、高劑量組的心肌組ACE2mRNA、表達(dá)分別高于生理鹽水對(duì)照組和低劑量組(p0.05,p0.01)。⑥8wk末白酒和酒精中、高劑量組的心肌組織ACEmRNA均高于生理鹽水對(duì)照組(p0.05,p0.01)。白酒、酒精高劑量組的心肌組織ACEmRNA表達(dá)分別高于各自的低劑量組、中劑量組(p0.01,p0.05)。 結(jié)果三：①12wk末白酒組、酒精組的各劑量組的LVP,HW/BW均比生理鹽水組升高(P0.05)。②在12wk末中,隨著時(shí)間延長,從低劑量組到高劑量組的心肌細(xì)胞胞漿渾濁、變性逐步加重,纖維組織逐步明顯增生,高劑量組還出現(xiàn)脂肪組織增生,各劑量組中以酒精組變化明顯。③12wk末白酒組和酒精組的低、中、高劑量組心肌組織AngⅡ、ACE含量均高于生理鹽水對(duì)照組(P0.05,P0.01,P0.01),且中、高劑量組的AngⅡ、ACE含量均維持在高水平,高于低劑量組(P0.01),而白酒高劑量組的心肌組織AngⅡ含量高于中劑量組(P0.01)。④12wk末時(shí)白酒組和酒精組的低劑量組心肌組織ACE2含量明顯高于生理鹽水對(duì)照組(P0.01),而中、高劑量組的ACE2含量均低于生理鹽水對(duì)照組(P0.01,P0.01)。⑤12wk末白酒和酒精低劑量組的心肌組ACE2mRNA、ACE2表達(dá)均高于生理鹽水對(duì)照組(p0.01)。白酒和酒精中、高組的的心肌組織ACE2mRNA表達(dá)均低于生理鹽水對(duì)照組(p0.05)。⑥12wk末白酒和酒精低、中、高劑量組的心肌組織ACEmRNA表達(dá)分別高于生理鹽水對(duì)照組(p0.01)白酒和酒精的中、高劑量組的心肌組織ACEmRNA表達(dá)分別高于各自的低劑量組(p0.05,p0.01)。白酒和酒精高劑量組的ACEmRNA表達(dá)分別高于各自的中劑量組(p0.05,p0.05) 結(jié)論：1.短期內(nèi)少量飲酒對(duì)心肌功能無明顯損傷,AngⅡ、ACE、ACE2含量無明顯差異。2.長期飲酒可導(dǎo)致心肌纖維化,其變化程度與飲酒量以及時(shí)間長短有關(guān)。3.長期飲酒可破壞心肌內(nèi)的ACE和ACE2平衡,使ACE和AngⅡ的升高,ACE2降低,導(dǎo)致心肌纖維化。4.在相同時(shí)間、相同劑量下,單純酒精對(duì)心臟的損傷重于食用白酒。
[Abstract]:Objective: in a certain period of time, the rats were continuously given different doses of white wine to observe the structure and function of the heart, and to detect the changes of ACE, ACE2 and Ang II in the myocardium.
Methods: 124 SD rats, according to 4wk, 8wk, 12wk three time points were randomly divided into liquor group, alcohol group, normal saline group, each group is divided into low, medium and high dose group, 6 rats in each group, a total of 27 groups. According to the low dose (0.25ml/100g/d), middle dose, high dose (0.5ml/100g/d) (1ml/100g/d), daily amount of two times (by each half) rats were given orally, two times more than 9h. The interval time weighed once a week, according to the weight adjusted the amount of gastric lavage for a week. Left ventricular pressure was determined respectively in the range of three time points (LVP), calculated the heart / body weight index (HW/BW), myocardial fibrosis was observed by Masson staining, detection of myocardial ELISA AAng II, ACE, ACE2 content, RT-PCR to detect the expression of myocardial cells of ACEmRNA and ACE2mRNA.
Results: the amplitude of 4wk at the end of a liquor group each dose group of left ventricular pressure, HW/BW and alcohol group, there was no significant difference between the normal saline group (P0.05). At the end of the 4wk liquor group, alcohol group, high dose group myocardial cells slightly turbid, degeneration, mild proliferation of fibrous tissue, and the dosage change obviously. The 4wk end liquor group, alcohol group each dose group of myocardial tissue of Ang, ACE, ACE2 content compared with the saline group, no significant difference (P0.05), but the increased tendency with the dose. The 4wk end liquor group, alcohol group the myocardial tissue in each dose group of ACEmRNA, the expression of ACE2mRNA compared with the normal saline group, no significant change (P0.05).
Results: at the end of two 8wk liquor group each dose group of LVP, HW/BW and alcohol group, there was no significant difference between the normal saline group (P0.05). At the end of 8wk, with the prolongation of time, from low dose to myocardial cells in high dose group gradually degenerated, muddy, fibrous tissue hyperplasia gradually obviously, each dose group in the alcohol group changed significantly. The 8wk at the end of the liquor, the high dose group and alcohol in the high dose group of myocardial tissue of Ang II, ACE concentrations were higher than those in the saline control group, low dose group (P0.05, P0.01), myocardial tissue of high dose group was higher than that of ACE and the content of alcohol the middle dose group (P0.05). The 8wk at the end of liquor and alcohol, low content of ACE2 in myocardial tissue of high dose group were higher than those in the saline control group (P0.05, P0.01, P0.01), middle dose group of liquor and alcohol, the content of ACE2 in myocardial tissue of high dose group and low dose group were higher than that of the respective (P0.05 P0.01), And the myocardial tissue of high dose group was higher than that in ACE2 medium dose group (P0.05). At the end of the 8wk liquor and alcohol group, myocardial ACE2mRNA high dose group, the expression were higher than that in the saline control group and low dose group (P0.05, P0.01). The 8wk at the end of liquor and alcohol, the high dose group of myocardial tissue ACEmRNA was higher than that of the saline control group (P0.05, P0.01). The expression of alcoholic liquor, the high dose group of ACEmRNA in the myocardial tissue were higher than the low dose group the middle dose group (P0.01, P0.05).
Results: at the end of three 12wk liquor group, alcohol group each dose group of LVP, HW/BW were higher than that of normal saline group (P0.05). At the end of 12wk, with the prolongation of time, from low dose to myocardial cells turbidity high dose group, degeneration increased gradually, gradually obvious hyperplasia of fibrous tissue also, the high dose group of fat tissue, each dose group in the alcohol group changed significantly. The 12wk at the end of liquor group and alcohol group, low and high dose group myocardial Ang II, ACE content were higher than the normal saline control group (P0.05, P0.01, P0.01), and in high dose group Ang II, ACE levels were maintained at a high level, higher than the low dose group (P0.01), and higher myocardial Ang content in liquor of high dose group middle dose group II (P0.01). The 12wk at the end of the myocardial tissue in low dose group ACE2 content of liquor group and alcohol group was significantly higher than the saline control group (P0.01) however, in high dose The content of ACE2 group were lower than the normal saline control group (P0.01, P0.01). The 12wk at the end of liquor and alcohol in low dose group myocardial ACE2mRNA group, the expression of ACE2 was higher than that of the saline control group (P0.01). The liquor and alcohol, high group of myocardial tissue ACE2mRNA expression were lower than the normal saline control group (P0.05 12wk). At the end of liquor and alcohol, low and high dose group of myocardial ACEmRNA expression were higher than those in the saline control group (P0.01) of liquor and alcohol in the high dose group of myocardial ACEmRNA expression were higher than the low dose group respectively (P0.05, P0.01). The liquor and alcohol in high dose group ACEmRNA the expression was higher than that of middle dose group respectively (P0.05, P0.05)
Conclusion: 1. in the short term, a small amount of alcohol had no obvious damage on myocardial function of Ang, ACE, ACE2 and.2. had no significant difference in the long-term drinking can lead to myocardial fibrosis, the degree of change and consumption as well as the related.3. long term alcohol consumption can damage the balance of cardiac ACE and ACE2, increased ACE, ACE2 and Ang II reduced, leading to myocardial fibrosis.4. at the same time, under the same dosage, simple injury to the heart in edible alcohol liquor.

【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363

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