本文關鍵詞：中國小腸結腸炎耶爾森菌在生豬中的分布及傳播方式的研究　出處：《中國疾病預防控制中心》2012年碩士論文　論文類型：學位論文

  更多相關文章： 小腸結腸炎耶爾森菌 生物型 血清型 脈沖場凝膠電泳 傳播途徑

【摘要】：小腸結腸炎耶爾森菌是一類在自然界廣泛分布的人獸共患病原菌,目前研究表明豬是其主要宿主,也是造成人類感染的主要來源。為了研究中國生豬中小腸結腸炎耶爾森菌的分布特征,于2009年3月至2011年11月期間選取位于中國中部的河南��；西南部的四川省、云南省；華北的北京市、內(nèi)蒙古自治區(qū)、天津市；東北的黑龍江�。晃鞅钡膶幭幕刈遄灾螀^(qū)、青海省和華東的江蘇省、江西省共11省區(qū)的大型屠宰場,養(yǎng)殖場及散養(yǎng)農(nóng)戶作為采樣地點共采集8773份標本,其中屠宰場宰殺生豬咽拭子標本4495份,回盲部腸內(nèi)容物標本1239份以及當?shù)仞B(yǎng)殖場或農(nóng)戶散養(yǎng)豬糞標本3039份。細菌分離結果表明,小腸結腸炎耶爾森菌的平均帶菌率從高到低依次為豬咽喉部19.53%,豬回盲部腸內(nèi)容物7.51%和豬糞便5.30%。血清分型結果顯示850株致病性菌株中除3株為2/0：9生物血清型,3株為4/0：3型外其他均為3/0：3型,可見0：3血清型已經(jīng)取代0：9型成為我國致病性小腸結腸炎耶爾森菌的主要型別。從北京、天津、江西、江蘇、寧夏回族自治區(qū)、內(nèi)蒙古自治區(qū)、四川省區(qū)豬咽拭子中分離到的小腸結腸炎耶爾森菌均以致病性的3/0：3型為主(85.17%-100%),相反在東北部的黑龍江省豬咽拭子中分離到的菌株僅有3.48%(4/115)為3/0：3型,96.52%(111/115)為非致病性的生物1A型多種血清型混雜的菌株。對所有致病性菌株進行脈沖場凝膠電泳分析,結果表明847株0：3型致病性小腸結腸炎耶爾森菌菌共分為49個帶型,其中以K6GN11C30021和K6GN11C30012帶型占多數(shù),分別為53.13%(450/847)和21.37%(181/847),且各個省市均有其獨特的PFGE帶型；同時通過聚類分析發(fā)現(xiàn)四川、江蘇、天津、河南等地腹瀉病人分離的致病性菌株與當?shù)刎i中分離株的主要帶型一致。病人中小腸結腸炎耶爾森菌的PFGE帶型組成與宿主動物豬、犬相似,而與其他家畜家禽則不大相同。 本研究結果表明中國多地區(qū)生豬中小腸結腸炎耶爾森菌的帶菌率均較高,分離菌株的生物血清型分布具有明顯地域性特征,并且通過分子生物學手段充分證實了豬的攜帶與病人感染的小腸結腸炎耶爾森菌密切相關。中國生豬養(yǎng)殖場和屠宰場分布廣泛,人群食用豬肉普遍,應警惕致病性小腸結腸炎耶爾森菌通過豬感染人和其它動物以及環(huán)境的危險。
[Abstract]:Yersinia enterocolitica is a kind of common zoonotic bacteria which are widely distributed in nature. At present, studies have shown that pigs are the main hosts and the main source of human infection. In order to study distribution characteristics of bacteria in the small intestine of the pig China Yersinia colitis, during the period from March 2009 to November 2011 were located in central Henan province China; southwestern Sichuan Province, Yunnan Province, Beijing City, North China; the Inner Mongolia Autonomous Region, Tianjin; Northeast Heilongjiang province; northwest of the Ningxia Hui Autonomous Region, Qinghai province and East China's Jiangsu Province, Jiangxi province a total of 11 provinces of the large slaughterhouses, farms and backyard farmers as the sampling sites were collected 8773 specimens, including the slaughter hog slaughter 4495 throat swab specimens, ileocecus intestinal contents of 1239 samples and local farms or farmers backyard pig manure samples were 3039. The results showed that the bacteria isolation, average carrier yersiniaenterocolitica rate from high to low was pig throat 19.53% pigs, ileocecus intestinal contents of 7.51% and 5.30% pig manure. Serotyping results showed that 850 strains of pathogenic strains in 3 strains of 2/0:9 serotype 3 strains for biological, 4/0:3 and others are of type 3/0:3, visible 0:3 serotype has replaced 0:9 in China has become the main type of pathogenic Yersinia enterocolitica. Enterocolitis Jerson bacteria isolated in pharyngeal swabs from Beijing, Tianjin, Jiangxi, Jiangsu, the Ningxia Hui Autonomous Region, the Inner Mongolia Autonomous Region, Sichuan provinces that are pig disease type 3/0:3 (85.17%-100%), in contrast to the pig in the northeast of Heilongjiang province from pharyngeal swab in strains to only 3.48% (4/115) 3/0:3, 96.52% (111/115) for hybrid non pathogenic biotype 1A multiple serotype strain. By pulsed field gel electrophoresis analysis of all pathogenic strains, the results showed that 847 strains of type 0:3 pathogenic fungus enterocolitis Jerson is divided into 49 types, among which the K6GN11C30021 and K6GN11C30012 band majority, were 53.13% (450/847) and 21.37% (181/847), and each province has its unique PFGE band at the same time; through cluster analysis found that the main type strain of Sichuan, Jiangsu, Tianjin, Henan and other places separated from the diarrhea patients of pathogenic strains and local pigs. In patients with intestinal bacteria Yersinia colitis with PFGE type composition and host animal swine, dogs and other animals are similar, and are not the same.
【學位授予單位】：中國疾病預防控制中心
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R378

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本文編號：1342814