轉(zhuǎn)Bt基因抗蟲棉生殖器官殺蟲蛋白低表達(dá)量影響抗蟲性。為提高生殖器官蕾花鈴殺蟲蛋白的表達(dá),以常規(guī)品種泗抗1號(hào)（s1）和雜交品種泗抗3號(hào)（s3）為試驗(yàn)材料,分別于2016年、2017年和2018年在以前研究基礎(chǔ)上設(shè)計(jì)構(gòu)成Bt蛋白主要成份的5種氨基酸和所有21種氨基酸處理噴灑蕾花鈴探討氨基酸外用對(duì)Bt棉生殖器官殺蟲蛋白表達(dá)量影響及相關(guān)的蛋白質(zhì)周轉(zhuǎn)機(jī)制。研究結(jié)果表明:與對(duì)照相比,兩個(gè)氨基酸處理均顯著提高了蕾的Bt蛋白含量,Bt蛋白含量增加7.4%-19.4%,全氨基酸應(yīng)用對(duì)棉蕾Bt蛋白含量提高的幅度更大主要與蛋白質(zhì)降解酶蛋白酶和肽酶活性下降幅度更大有關(guān)。氨基酸處理顯著提高花中Bt蛋白含量,比對(duì)照提高了15.2%～25.8%。但兩個(gè)氨基酸處理間差異不顯著。相似地,2個(gè)氨基酸處理的鈴殼、棉子和纖維中Bt蛋白也顯著提高。如花后25天,泗抗1號(hào)Bt蛋白含量量鈴殼中提高了 7.4%%-9.30%、棉子中高了23.9%-71.1%,纖維中提高了 23.8%-172.0%;泗抗3號(hào)Bt蛋白含量量鈴殼中提高了39.0-41.3%、棉子中提高了 15.8%-53.8%、纖維中提高了 20.0%-230.3%。此... 

【文章來(lái)源】：揚(yáng)州大學(xué)江蘇省

【文章頁(yè)數(shù)】：50 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
Abstract
中文摘要
1. Introduction
2. Materials and Methods
    2.1 Materials and experimental design
    2.2 Preparation of plant material
        2.2.1 Sampling
            2.2.1.1 The cry IAc protein content
            2.2.1.2 Free amino acid and soluble protein content
            2.2.1.3 Glutamic-pyruvic transaminase (GPT) and glutamate oxaloacetatetransaminase (GOT)
            2.2.1.4 Protease and peptidase activity
    2.3 Statistics analysis
3. Results
    3.1 The amino acids application treatments on the square insecticidal property
        3.1.1 On the square insecticidal protein concentration
        3.1.2 The amino acids application treatments on square nitrogen metabolismcharacteristics
            3.1.2.1 On square GPT and GOT activity
            3.1.2.3 On the square amino acid and soluble protein content
        3.1.3 Relationship between the square insecticidal protein concentration and nitrogenmetabolism
    3.2 The amino acids application treatments on the flower insecticidal property
        3.2.1 On the flower Bt protein concentration
        3.2.2 On the flower nitrogen metabolism characteristics
            3.2.2.1 On flower GPT and GOT activity
            3.2.2.2 On flower protease and peptidase activity
            3.2.2.3 On the flower amino acid and soluble protein content
        3.2.3 Relationship between the flower insecticidal protein concentration and nitrogenmetabolism
    3.3 The amino acids application treatments on the boll insecticidal property
        3.3.1 On the boll shell insecticidal property
            3.3.1.1 on boll shell Bt protein concentration
            3.3.1.2 on boll shell nitrogen metabolism characteristics
                3.3.1.2.1 On boll shell GPT and GOT activity
                3.3.1.2.2 On boll shell protease and peptidase activity
                3.3.1.2.3 On the boll shell amino acid and soluble protein content
            3.3.1.3 Relationship between the boll shell insecticidal protein concentration andnitrogen metabolism
        3.3.2 The amino acids application treatments on the seeds insecticidal property
            3.3.2.1 On the Cotton seeds insecticidal protein concentration
            3.3.2.2 on seeds nitrogen metabolism characteristics
                3.3.2.2.1 On the seeds GPT and GOT activity
                3.3.2.2.2 On seeds protease and peptidase activity
                3.3.2.2.3 On the seeds amino acid and soluble protein content
            3.3.2.3 Relationship between the seeds insecticidal protein concentration andnitrogen metabolism
        3.3.3 The amino acids application treatments on the fiber insecticidal property
            3.3.3.1 On fiber insecticidal protein concentration
                3.3.3.1.1 On seeds GPT and GOT activity
                3.3.3.1.2 On fiber protease and peptidase activity
                3.3.3.1.3 On fiber soluble protein and amino acid content
            3.3.3.2 Relationship between the fiber insecticidal protein concentration andnitrogen metabolism
4. Discussion
    4.1 Amino acid application enhanced square, flower and boll Bt protein concentration in Btcotton
    4.2 Increased protein synthesis and reduced protein degradation by exterior amino acidapplication caused elevated Bt toxin content in Bt cotton
5. Conclusions
6. References
Acknowledgement
The publications of the author


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