【摘要】：用不同電場條件(電場頻率為4～6kHz,電場強度為2～5kV/cm,處理時間為5min和l0min)對糜子種子進行處理后,采用濃度為16%的聚乙二醇6000溶液模擬干旱脅迫,實驗測定糜子萌發(fā)期發(fā)芽率、胚根長、胚芽長和根干重等,篩選顯著提高糜子種子抗旱性的電場處理條件;利用篩選所得電場條件處理糜子種子,測定干旱脅迫下幼苗的株高、根長、葉綠素含量和超氧化物歧化酶(SOD)活力等指標,分析電場處理條件對幼苗抗旱性的影響;用篩選出的電場條件處理糜子種子后,分別在當天,貯存7天,14天和21天進行萌發(fā)實驗,測定干旱脅迫下糜子種子的發(fā)芽勢和發(fā)芽率,探究電場對糜子影響的時效性。本實驗測定不同電場頻率,不同電場強度及不同處理時間對植物生長影響的規(guī)律,旨在找出顯著提高植物抗旱性的電場條件,為電生物技術(shù)在農(nóng)業(yè)生產(chǎn)中的開發(fā)利用提供理論和實驗依據(jù)。實驗結(jié)果表明:與自然對照組比較,干旱脅迫使糜子種子發(fā)芽率、胚根長及胚芽長均降低,下降幅度分別為17.84%、65.58%及67.41%,胚根干重與胚芽干重則顯著增加,增長幅度分別為34.63%與23.25%;電場處理糜子種子,可降低干旱脅迫對發(fā)芽率、胚根長及胚芽長的不利影響,使得胚根干重和胚芽干重整體保持上升趨勢,胚根干重和胚芽干重增長幅度最高可達58.45%和44.02%。電場處理對干旱脅迫下糜子幼苗的株高、根長、根鮮重與根干重有不同程度的促進作用,它們相對干旱脅迫對照組的增長率分別為25.33%、37.73%、217.19%和158.54%。實驗還發(fā)現(xiàn),電場處理糜子種子的生物效應(yīng)存在時效性,糜子種子經(jīng)電場處理后,貯存不同時間,糜子種子的發(fā)芽勢和發(fā)芽率產(chǎn)生不同變化,隨著貯存時間的推移,電場處理糜子種子產(chǎn)生的影響逐漸消失。
[Abstract]:The seeds were treated with different electric field conditions (electric field frequency of 4 ~ 6 kHz, electric field intensity of 2 ~ 5 kV / cm, treatment time of 5min and l0min). After treatment, 16% polyethylene glycol 6000 solution was used to simulate drought stress. The germination rate, radicle length, germ length and root dry weight were determined in order to select the electric field treatment conditions to improve the drought resistance of millet seeds. The plant height, root length, chlorophyll content and (SOD) activity of superoxide dismutase (SOD) were measured under drought stress, and the effects of electric field treatment on drought resistance were analyzed. The germinating experiments were carried out on the same day, 7 days, 14 days and 21 days after the treatment with the selected electric field conditions. The germinating potential and germination rate of the millet seeds under drought stress were measured, and the time effect of electric field on the mildew was investigated. The effects of different electric field frequencies, different electric field intensity and different treatment time on plant growth were determined in order to find out the electric field conditions that could significantly improve the drought resistance of plants. It provides theoretical and experimental basis for the development and utilization of electric biotechnology in agricultural production. The results showed that: compared with the natural control group, drought stress decreased the germination rate, radicle length and germ length of mycorrhiza hygrophylla, which decreased by 17.84% and 67.41%, respectively. The dry weight of radicle and dry weight of germ increased significantly. The increase was 34.63% and 23.25% respectively. When treated with electric field, the adverse effects of drought stress on germination rate, radicle length and germ length could be reduced, and the dry weight of radicle and dry weight of embryo kept a rising trend as a whole. The maximum increase range of dry weight of radicle and embryo was 58.45% and 44.02% respectively. The plant height, root length, root fresh weight and root dry weight were promoted by electric field treatment, and the growth rates of them were 25.33 ~ 37.73% and 158.54%, respectively. It was also found that the biological effects of electric field treatment were time-dependent. After stored in different time, the germination potential and germination rate of mycorrhiza mycorrhizae seeds varied with the storage time. The effect of electric field treatment on mildew seeds gradually disappeared.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S516

【參考文獻】

相關(guān)期刊論文 前10條

1 朱世秋;張琳雪;陳周;徐艷;王夢迪;閆建河;劉濱疆;;高壓電場對植物電特性的影響及促進光合作用的室內(nèi)試驗[J];農(nóng)業(yè)工程學(xué)報;2016年17期

2 張宇;王素萍;馮建英;;2015年秋季全國干旱狀況及其影響與成因[J];干旱氣象;2015年06期

3 趙宇;劉猛;劉斐;夏雪巖;南春梅;李順國;王慧軍;;中國谷子糜子產(chǎn)業(yè)發(fā)展趨勢及建議[J];農(nóng)業(yè)展望;2015年03期

4 張美俊;楊武德;喬治軍;馮美臣;王冠;段云;陳凌;;不同糜子品種萌發(fā)期對干旱脅迫的響應(yīng)及抗旱性評價[J];草地學(xué)報;2013年02期

5 馮曉敏;張永清;;水分脅迫對糜子植株苗期生長和光合特性的影響[J];作物學(xué)報;2012年08期

6 蔣耀庭;常秀蓮;李磊;;高壓靜電場處理對鮮切青花菜保鮮的影響[J];食品科學(xué);2012年12期

7 張盼盼;馮佰利;王鵬科;高小麗;高金鋒;宋慧;張小東;柴巖;;PEG脅迫下糜子苗期抗旱指標鑒選研究[J];中國農(nóng)業(yè)大學(xué)學(xué)報;2012年01期

8 張盼盼;馮佰利;王鵬科;高小麗;高金鋒;柴巖;宋慧;;糜子芽期抗旱性指標鑒選與利用研究[J];河北農(nóng)業(yè)科學(xué);2010年11期

9 王敏杰;;作物抗旱性生理生化鑒定指標的研究現(xiàn)狀[J];遼寧農(nóng)業(yè)職業(yè)技術(shù)學(xué)院學(xué)報;2010年05期

10 杜磊;趙尊練;鞏振輝;郭建偉;牛哲輝;郭永青;;水分脅迫對線辣椒葉片滲透調(diào)節(jié)作用的影響[J];干旱地區(qū)農(nóng)業(yè)研究;2010年03期

相關(guān)碩士學(xué)位論文 前5條

1 劉翔宇;不同電場條件處理檸條種子對干旱條件下幼葉基因表達的影響[D];內(nèi)蒙古大學(xué);2015年

2 汪金鵬;電場處理檸條種子對抗旱性影響的顯著性條件[D];內(nèi)蒙古大學(xué);2011年

3 張盼盼;糜子抗旱性鑒定及抗旱指標篩選[D];西北農(nóng)林科技大學(xué);2010年

4 閆淑春;我國干旱災(zāi)害影響及抗旱減災(zāi)對策研究[D];中國農(nóng)業(yè)大學(xué);2005年

5 齊永青;不同小麥品種的抗旱生理特性及其抗旱性評價的研究[D];河北農(nóng)業(yè)大學(xué);2003年

，

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