【摘要】：馬鈴薯黃萎病是近年來影響馬鈴薯產(chǎn)量和品質(zhì)的主要病害之一,為了了解我國北方馬鈴薯產(chǎn)區(qū)馬鈴薯黃萎病的發(fā)生情況和病原菌種類,我們在2013-2015年在內(nèi)蒙古、黑龍江、吉林、河北、山西、甘肅等馬鈴薯產(chǎn)區(qū)采集馬鈴薯病株,對(duì)其進(jìn)行組織分離和菌種的鑒定。共分離到馬鈴薯黃萎病菌菌株132個(gè),根據(jù)菌株形態(tài)及利用ITS和特異性引物鑒定,結(jié)果表明其中有72株大麗輪枝菌Verticilliumdahliae和60株黑白輪枝菌Verticilliumal6o-atrum。同時(shí)本試驗(yàn)利用ISSR標(biāo)記方法對(duì)采集到的兩種黃萎病菌分別進(jìn)行遺傳多樣性分析,分析其親緣關(guān)系與地理分布和致病力分化的相關(guān)性規(guī)律。采自不同地區(qū)的大麗輪枝菌與黑白輪枝菌的種內(nèi)親緣關(guān)系結(jié)果均表明,烏蘭察布地區(qū)的菌株遺傳距離與錫林郭勒盟、黑龍江、吉林、河北壩上幾個(gè)地區(qū)的菌株遺傳距離較遠(yuǎn);基于不同致病力菌株的遺傳多樣性也表明,無論大麗輪枝菌還是黑白輪枝菌,強(qiáng)致病力菌株和中等致病力菌株組沒有顯著的遺傳差異,但是弱致病力菌株與中等或強(qiáng)致病力菌株組均有顯著性差異。生防菌篩選試驗(yàn)結(jié)果表明,菌株XT1-4對(duì)馬鈴薯黃萎病有明顯的防治效果且防治效果在60%以上,應(yīng)用16S rDNA序列分析,并結(jié)合其形態(tài)學(xué)以及革蘭氏染色等生理生化指標(biāo)的測定,將菌株XT1-4鑒定為特基拉芽孢桿菌Bacillus tequilensis。
[Abstract]:Verticillium wilt is one of the major diseases affecting the yield and quality of potato in recent years. In order to understand the occurrence of Verticillium wilt and the kinds of pathogenic bacteria in potato producing areas in northern China, we have been in Inner Mongolia, Heilongjiang Province from 2013 to 2015. Potato diseased plants were collected from potato producing areas in Jilin, Hebei, Shanxi and Gansu provinces. 132 strains of Verticillium dahliae were isolated and identified by ITS and specific primers. The results showed that 72 strains of Verticilliumdahliae and 60 strains of Verticilliumal6o-atrum. were isolated from Verticillium dahliae. At the same time, the genetic diversity of two species of Verticillium wilt collected was analyzed by ISSR marker method, and the correlation of genetic relationship with geographical distribution and pathogenicity differentiation was analyzed. The results showed that the genetic distance of the strains in Wulanchabu region was far from that in Xilingol League, Heilongjiang, Jilin and Bashang of Hebei. Based on the genetic diversity of different virulence strains, there was no significant genetic difference between the strong pathogenic strains and the moderate pathogenic strains, regardless of the number of rotifers or black-and-white rotifers. However, there were significant differences between weak virulence strains and moderate or strong virulence strains. The results of biocontrol screening test showed that XT1-4 had obvious control effect on Verticillium wilt of potato and the control effect was more than 60%. The 16s rDNA sequence analysis was used, and the physiological and biochemical indexes such as morphology and Gram staining were determined. Identification of Bacillus Tequila Bacillus tequilensis. by XT1-4
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S435.32

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