南平市豬繁殖與呼吸綜合征血清學(xué)和病原學(xué)的監(jiān)測分析
發(fā)布時間：2018-11-02 16:20
【摘要】：豬繁殖與呼吸綜合征(英文簡稱PRRS)又稱"藍耳病",由豬繁殖與呼吸綜合征病毒(PRRSV)引起。患病動物以妊娠母豬和仔豬最為常見,且感染后容易發(fā)生混合感染,給養(yǎng)豬業(yè)帶來巨大經(jīng)濟損失,屬于我國的一類疫病。為了因地制宜制定豬藍耳病防控方法,本課題擬對南平市PRRSV發(fā)病與強制免疫情況進行系統(tǒng)分析。初步掌握全市高致病性藍耳病的抗體水平,并結(jié)合病原學(xué)調(diào)查分析深層次因素,為防治豬藍耳病提供理論依據(jù)。在2014-2016年期間,我們在南平市全市范圍內(nèi)60個規(guī)模養(yǎng)殖場和90個散養(yǎng)戶中隨機挑選2040頭接種藍耳疫苗后一個月的健康豬,前腔靜脈采集血樣,分離血清,采用ELISA檢測方法檢測豬藍耳病抗體,其中規(guī)模養(yǎng)殖場采樣1500頭,散養(yǎng)戶采樣540頭。結(jié)果顯示,全市共有1845份血清樣品的PRRSV抗體陽性,合格率為90.4%;其中,規(guī)�；B(yǎng)豬場共有1414份血清呈PRRSV抗體陽性,合格率為94.3%;散養(yǎng)式豬場共有431份血清呈PRRSV抗體陽性,合格率為79.8%。從2014-2016年期間,全市每年的豬藍耳病抗體合格率依次為88.7%、90.4%和92.2%,呈現(xiàn)逐年遞增的趨勢。同時,我們于2014-2016年期間采集規(guī)模養(yǎng)殖場和散養(yǎng)戶的豬鼻拭子并提取RNA,采用RT-PCR方法檢測其中PRRSV,結(jié)果顯示,所有待檢樣品均未檢測到PRRSV,這一結(jié)果說明所檢測的規(guī)模養(yǎng)豬場和散養(yǎng)戶中暫無豬藍耳病病毒傳播,其所在一定區(qū)域內(nèi)防控工作開展較好。綜上所述,南平市全市在2014-2016年期間的豬只PRRSV抗體合格率呈逐年遞增趨勢,且規(guī)模化養(yǎng)殖場的PRRSV抗體合格水平高于散養(yǎng)戶。同時,在調(diào)查期間,所有檢測樣品均未檢出PRRSV。這提示全市在2014-2016年期間無PRRS疫情,相對于散養(yǎng)戶,規(guī)模養(yǎng)殖場能夠更嚴格執(zhí)行免疫計劃。
[Abstract]:Porcine reproductive and respiratory syndrome (PRRS), also called blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (PRRSV). Pregnant sows and piglets are the most common diseased animals, and the mixed infection is easy to occur after infection, which brings huge economic losses to the pig industry and belongs to a class of epidemic diseases in China. In order to make the prevention and control method of pig blue ear disease according to local conditions, this paper systematically analyzed the incidence and compulsory immunity of PRRSV in Nanping city. In order to provide theoretical basis for the prevention and treatment of blue ear disease in pigs, the antibody level of high pathogenic blue ear disease in the whole city was preliminarily grasped and the underlying factors were analyzed in combination with etiological investigation. During 2014-2016, we randomly selected 2040 healthy pigs one month after they were vaccinated against blue ear in 60 large-scale farms and 90 free-range households in Nanping City. Blood samples were collected from the anterior vena cava to isolate serum. ELISA detection method was used to detect antibodies to blue ear disease in pigs. Among them, 1500 were sampled in scale farms and 540 in scattered households. The results showed that 1845 serum samples were positive for PRRSV antibody, and the qualified rate was 90.4. Among them, 1414 sera from large-scale pig farms were positive for PRRSV antibody, and the qualified rate was 94.3%. A total of 431 sera were positive for PRRSV antibody, and the qualified rate was 79.8%. From 2014-2016, the qualified rate of antibody against blue ear disease of pigs in the whole city was 88.70.40% and 92.2% respectively, which showed an increasing trend year by year. At the same time, during 2014-2016, we collected pig swabs from large-scale farms and scattered farmers and extracted RNA,. The results of PRRSV, detection by RT-PCR method showed that no PRRSV, was detected in all the samples to be tested. The results showed that there was no transmission of blue ear disease virus in pig farms and free-range farms, and the prevention and control work was well carried out in a certain area. To sum up, the qualified rate of PRRSV antibody of pigs in Nanping city from 2014-2016 is increasing year by year, and the qualified level of PRRSV antibody in large-scale breeding farm is higher than that in retail farmers. At the same time, no PRRSV. was detected in all samples during the investigation. This suggests that there will be no PRRS outbreak in the city between 2014-2016 and that scale farms will be able to enforce immunization programs more strictly than free-range farmers.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.28
本文編號：2306316
[Abstract]:Porcine reproductive and respiratory syndrome (PRRS), also called blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (PRRSV). Pregnant sows and piglets are the most common diseased animals, and the mixed infection is easy to occur after infection, which brings huge economic losses to the pig industry and belongs to a class of epidemic diseases in China. In order to make the prevention and control method of pig blue ear disease according to local conditions, this paper systematically analyzed the incidence and compulsory immunity of PRRSV in Nanping city. In order to provide theoretical basis for the prevention and treatment of blue ear disease in pigs, the antibody level of high pathogenic blue ear disease in the whole city was preliminarily grasped and the underlying factors were analyzed in combination with etiological investigation. During 2014-2016, we randomly selected 2040 healthy pigs one month after they were vaccinated against blue ear in 60 large-scale farms and 90 free-range households in Nanping City. Blood samples were collected from the anterior vena cava to isolate serum. ELISA detection method was used to detect antibodies to blue ear disease in pigs. Among them, 1500 were sampled in scale farms and 540 in scattered households. The results showed that 1845 serum samples were positive for PRRSV antibody, and the qualified rate was 90.4. Among them, 1414 sera from large-scale pig farms were positive for PRRSV antibody, and the qualified rate was 94.3%. A total of 431 sera were positive for PRRSV antibody, and the qualified rate was 79.8%. From 2014-2016, the qualified rate of antibody against blue ear disease of pigs in the whole city was 88.70.40% and 92.2% respectively, which showed an increasing trend year by year. At the same time, during 2014-2016, we collected pig swabs from large-scale farms and scattered farmers and extracted RNA,. The results of PRRSV, detection by RT-PCR method showed that no PRRSV, was detected in all the samples to be tested. The results showed that there was no transmission of blue ear disease virus in pig farms and free-range farms, and the prevention and control work was well carried out in a certain area. To sum up, the qualified rate of PRRSV antibody of pigs in Nanping city from 2014-2016 is increasing year by year, and the qualified level of PRRSV antibody in large-scale breeding farm is higher than that in retail farmers. At the same time, no PRRSV. was detected in all samples during the investigation. This suggests that there will be no PRRS outbreak in the city between 2014-2016 and that scale farms will be able to enforce immunization programs more strictly than free-range farmers.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.28
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