藥劑種子處理對(duì)玉米幼苗化學(xué)防御反應(yīng)的影響及機(jī)理初探
[Abstract]:Plant defense is a ubiquitous phenomenon in the plant community, which is the main form of plant resistance to environmental stress. Plant defense is controlled by genetic factors, but under the guidance of different environmental factors, different defense responses can be generated. Plant chemical defense is mainly to resist environmental stress by regulating the activity of defense enzymes and the content of secondary metabolites in vivo. This paper deals with the changes of the defense system in plant seedlings when two kinds of suspension seed dressing agents were used to treat the three maize varieties, namely, Yuyu 35, Shengrui 999 and Oyu 21, respectively. The effects of these two kinds of suspending agents on the maize defense system were studied by measuring the activities of CAT, POD and PPO and the changes of plant defense substances (p-Hydroxybenzoic acid, caffeic acid, cinnamic acid, Syringic acid, Xiangdou acid, water-soluble acid, ferulic acid and fragrant oxalic acid). At the same time, the changes of gene expression of plant defense related substances (butylphenol, phenolic acid and defense protein) were detected, and the changes of plant defense ability were analyzed. This is of practical significance to guide the use of anti-virus agent in combination with plant's defense ability, and to improve the insect-resistant and disease-resistant ability of plants. The main results of this experiment were as follows: (1) The activity of catalase in plant seedlings was obviously increased after the treatment with oxytetracycline or pentosan alcohol, and the activity of catalase in plant seedlings increased by 3. 6 times, 2. 3 and 2. 8 times higher than that of the control group. (2. 4 times, 3. 2 and 2. 3 times respectively) were increased after the use of pentode alcohol. The activities of POD and PPO were relatively complex, and the activity of POD was increased by 62. 6% and the activity of POD in other treatment groups decreased at day 8. However, POD activity gradually approached the activity of control group on day 18. The PPO activity increased by 37. 5% on day 8 and PPO activity in other treatment groups decreased, and the PPO activity decreased by 33.3% at the 12th day. The activity of PPO decreased by 26. 3% on day 8. (2) The content of Syringic acid and ferulic acid in the five phenolic acids detected was significantly lower than that of the control group at day 8, and the contents of the five phenolic acids were decreased by 10. 31 ug/ g and 4. 3 ug/ g respectively on day 8, and the content of the control group was close to the control group at day 18. The content of caffeic acid and ferulic acid decreased by 3.39 & mu; g/ g and 6.31 & mu; g/ g after use of sodium pentode, and the content of caffeic acid recovered gradually on day 18. The content of five phenolic acids was higher than that of the control group after the use of carvedilol. The content of caffeic acid and ferulic acid in the seedlings of St. Rainy's 999 seedlings decreased, but returned to normal level at day 10. After treatment of Oyu 21, the content of five phenolic acids decreased, in which the content of clove acid and ferulic acid was the largest, and the content of five phenolic acids decreased by 2.64 ug/ g and 3.09 ug/ g at 18 days. In addition, the content of ferulic acid in the seedling was decreased, and the content of other four phenolic acids increased in short time. (3) The expression levels of Bx1, Bx6 and PR-2a were decreased by 3.89, 3.67 and 1. 5 times respectively on day 18, and the expression levels of other genes were up-regulated. The expression of Bx6, MPI, TPS and FPS was decreased by 4.04 fold, 2.09 times, 1. 15 fold and 2.43 fold, 2. 09 times, 1. 15 fold and 2. 43 fold, 2. 09 times, 1. 15 fold, and 2. 43 fold, 2. 09 times, 1. 15 fold and 2. 43 fold, and the total phenol synthesis was increased. The synthesis of direct defense protein and indirect defense material was increased, and the expression of PR-2a was decreased by 3. 01 times on 14 days after use of pentode alcohol. only the PAL expression is down regulated by 3.48 times, the synthesis of the butylphenol, the direct defense protein and the indirect defense material is promoted, the synthesis of the total phenol is inhibited, It can promote the synthesis of total phenol and direct defense protein.
【學(xué)位授予單位】:河南科技學(xué)院
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類號(hào)】:S513;S351.1
【參考文獻(xiàn)】
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