【摘要】：大豆生育期和株型對(duì)大豆產(chǎn)量具有重要影響。本文通過(guò)春夏播研究了不同播期對(duì)大豆生育期的影響,并對(duì)大豆葉柄長(zhǎng)度相關(guān)QTL位點(diǎn)進(jìn)行了初步定位。在播期對(duì)不同大豆種質(zhì)生育期的影響方面,本研究以來(lái)自國(guó)內(nèi)不同生態(tài)區(qū)的57份大豆種質(zhì)和來(lái)自國(guó)外的10份大豆種質(zhì)為研究材料,分春夏兩季進(jìn)行播種,在整個(gè)生育期調(diào)查生育期相關(guān)性狀。研究結(jié)果表明,春播條件下,與原生育期相比,16份種質(zhì)的生育期縮短、12份相差5 d之內(nèi)、39份生育期延長(zhǎng)。生育期縮短的種質(zhì)中,綏農(nóng)6號(hào)縮短天數(shù)最多,達(dá)33 d;生育期延長(zhǎng)的種質(zhì)中,盤石豆延長(zhǎng)天數(shù)最多,達(dá)54 d。夏播條件下,與原生育期相比,27份種質(zhì)的生育期縮短、28份相差5 d之內(nèi)、12份生育期延長(zhǎng)。生育期縮短的種質(zhì)中,吉育30縮短天數(shù)達(dá)55 d。與春播相比,夏播時(shí),大豆全生育期縮短,主要是生育前期的縮短,即播種至結(jié)莢階段的縮短。從積溫角度來(lái)說(shuō),夏播大豆比春播大豆全生育期所需積溫減少,主要是開(kāi)花和結(jié)莢期的減少,而在其他發(fā)育階段兩者差異不明顯。以兩個(gè)具有相同遺傳背景的且葉柄長(zhǎng)度差異明顯的回交導(dǎo)入系作為親本配制定位群體,對(duì)大豆葉柄長(zhǎng)度QTL進(jìn)行了初步定位。經(jīng)過(guò)對(duì)F2群體進(jìn)行表型分析及基因分型,運(yùn)用IciMapping軟件,將大豆葉柄長(zhǎng)度相關(guān)基因定位到2號(hào)染色體Satt290引物所在的D1b連鎖群上,LOD值為2.6632。
[Abstract]:Soybean growth period and plant type have important influence on soybean yield. In this paper, the effects of different sowing dates on soybean growth period were studied by sowing in spring and summer, and the QTL loci related to petiole length of soybean were preliminarily mapped. In terms of the effect of sowing date on the growth period of soybean germplasm, 57 soybean germplasms from different ecological areas in China and 10 soybean germplasms from abroad were used as materials for sowing in spring and summer. The related traits of growth period were investigated during the whole growth period. The results showed that under the condition of spring sowing, the growth period of 16 germplasms was shorter than that of the original germplasm, and 39 germplasms had a longer growth period within 5 days of the difference of 12 germplasms. Among the germplasm with shorter growth period, Suinong 6 shortened the most days, up to 33 days, and among the germplasm with longer growth period, the most extended days were Panshi bean (54 days). Under the condition of summer sowing, the growth period of 27 germplasms was shorter than that of the original germplasm, and the growth period of 12 germplasms was prolonged within 5 days of the difference of 28 germplasms. Among the germplasm with shorter growth period, Jiyu 30 shortened the days to 55 days. Compared with spring sowing, the whole growth period of soybean was shortened during summer sowing, mainly the shortening of early growing stage, that is, the shortening of sowing stage to pod-setting stage. From the point of view of accumulated temperature, the required accumulated temperature of summer sowing soybean was lower than that of spring sowing soybean in the whole growth period, mainly the decrease of flowering and pod-setting stage, but there was no significant difference between them in other developmental stages. Two backcross lines with the same genetic background and significant difference in petiole length were used as parent to prepare the localizing population. The QTL of petiole length of soybean was preliminarily mapped. After phenotypic analysis and genotyping of F2 population, IciMapping software was used to localize the length related gene of petiole to the D1b linkage population on chromosome 2. The Lod value was 2.6632.
【學(xué)位授予單位】：聊城大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S565.1

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