【摘要】：天麻是我國傳統(tǒng)名貴中藥之一,為蘭科植物天麻(Gastrodia elata B1.)的干燥塊莖。具有息風(fēng)止痙,平抑肝陽,祛風(fēng)通絡(luò)的功效。臨床上多用于小兒驚風(fēng),癲癇抽搐,破傷風(fēng),頭痛眩暈,肢體麻木,風(fēng)濕痹痛等癥。研究還發(fā)現(xiàn),天麻具有抗驚厥、鎮(zhèn)靜、鎮(zhèn)痛、改善供血、增加腦血流量等多種藥理活性。四川滎經(jīng)縣是四川省確認的天麻適宜生態(tài)區(qū),種植的天麻有滎經(jīng)“明麻”之盛名,其產(chǎn)品2012年獲得了國家農(nóng)產(chǎn)品地理標志,在藥材商品中享有較高的聲譽。本試驗以滎經(jīng)天麻為研究對象,收集其野生、家種、引種樣品共25個,以國內(nèi)主產(chǎn)區(qū)所產(chǎn)天麻作對比,對其天麻素、天麻多糖、氨基酸、微量元素等的含量進行了測定,并初步繪制了滎經(jīng)天麻的HPLC指紋圖譜。采用HPLC法對的天麻素含量進行測定,測得滎經(jīng)天麻的天麻素含量在0.157%-0.236%之間,平均值為0.21%,達到藥典(2010版)0.2%的要求；以苯酚-濃硫酸法測定天麻中天麻多糖的含量,測得滎經(jīng)天麻的天麻總多糖含量在12.21%-31.29%之間,平均含量為22.97%,建議將滎經(jīng)天麻含量不低于20%作為質(zhì)量評價的指標；以氨基酸自動分析儀對天麻中的氨基酸含量進行測定,測得滎經(jīng)天麻的氨基酸含量在230.80 mg/g-375.39 mg/g之間,平均氨基酸總含量為288.65mg/g,建議將氨基酸總含量不低于230.80mg/g作為質(zhì)量評價的指標；采用火焰原子吸收光譜儀測定天麻的微量元素含量,測得滎經(jīng)縣天麻中含有常見的微量元素,如Fe、Zn、 Cu、Mg、Mn、Co等,尤其以Fe、Mn的含量較高,建議作為滎經(jīng)天麻的特征元素指標；初步建立了滎經(jīng)天麻成分的HPLC指紋圖譜。這樣系統(tǒng)研究滎經(jīng)天麻尚屬首次,為全面評價滎經(jīng)天麻質(zhì)量奠定了一定基礎(chǔ)。
[Abstract]:Gastrodia elata B1. is one of the precious traditional Chinese medicines. It has the effect of relieving wind and spasm, calming liver yang, dispelling wind and dredging collaterals. Xingjing County, Sichuan Province, is a suitable ecological region for Gastrodia elata. The cultivated Gastrodia elata is well-known as "Mingma" by Xingjing. Its products won the national geographical indications of agricultural products in 2012 and enjoy a high reputation in medicinal commodities. The contents of gastrodin, gastrodin polysaccharide, amino acid, trace element, etc. in the main producing areas of China were determined, and the fingerprint of Xingjing Gastrodia elata by HPLC was preliminarily drawn. The content of Gastrodin in Xingjing Gastrodia elata was determined by HPLC. The content of Gastrodin in Xingjing Gastrodia elata was 0.1. The content of polysaccharide in Gastrodia elata was determined by phenol-concentrated sulfuric acid method. The total polysaccharide content of Gastrodia elata was between 12.21% and 31.29%, and the average content was 22.97%. It was suggested that the content of Gastrodia elata elata elata elata should be no less than 20% as the quality evaluation index. The content of amino acids in Gastrodia elata was determined by automatic analyzer. The content of amino acids in Gastrodia elata was 230.80 mg/g-375.39 mg/g and the average content of amino acids was 288.65 mg/g. It was suggested that the total content of amino acids should not be less than 230.80 mg/g as the quality evaluation index. The content of trace elements such as Fe, Zn, Cu, Mg, Mn and Co in Gastrodia elata from Xingjing County was determined. Especially the content of Fe and Mn was high. It was suggested that the content of these elements should be taken as the characteristic element index of Gastrodia elata. The HPLC fingerprint of the components of Gastrodia elata elata elata was preliminarily established. Fixed a certain foundation.
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S567.239

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本文編號：2219884