本文選題：俄羅斯鱘 + 養(yǎng)殖密度　； 參考：《中國海洋大學》2015年碩士論文

【摘要】：為研究流水養(yǎng)殖系統(tǒng)中不同養(yǎng)殖密度對俄羅斯鱘(Acipenser gueldenstaedti)幼魚生長、血液生理生化以及非特異性免疫功能的影響,實驗選取初始體重為(29.70±1.32)g的俄羅斯鱘幼魚分置于2.5kg/m3 (SD1)、3.6kg/m3 (SD2)和4.7kg/m3 (SD3) 3個養(yǎng)殖密度進行流水池塘(4.4×4.4×0.45 m3)養(yǎng)殖,每個密度設3個重復,實驗周期為90d。實驗結果如下：1、低密度組(SD1)幼魚增重率(WG)、體長增長率(LG)、特定生長率(SGR)和餌料轉化率(FCR)分別為：362.01士15.87、55.88±4.77、1.79±0.03、114.95±4.52,顯著高于高密度組(SD3)幼魚相應指標(272.30±2.74、46.34±6.22、1.53±0.02、94.49±1.96)(P0.05),而SD3組幼魚生長離散程度和死亡率顯著高于SD1組幼魚(P0.05)；隨養(yǎng)殖密度增加,幼魚蛋白和脂肪含量降低,水分和灰分含量升高,但是不存在顯著性差異(P0.05)。研究表明,較高的養(yǎng)殖密度對俄羅斯鱘幼魚的生長造成了顯著的負面影響。2、高養(yǎng)殖密度慢性脅迫對俄羅斯鱘幼魚的生理生化指標均產生一定的抑制作用,血紅蛋白含量與養(yǎng)殖密度呈顯著正相關。不同養(yǎng)殖密度中俄羅斯鱘幼魚的白細胞含量具有顯著性差異,SD3組幼魚血液中白細胞數(shù)量較SD1、SD2組大幅增多(P0.05),這表明長期高密度養(yǎng)殖對俄羅斯鱘幼魚產生一定程度的病理性損傷。各密度組幼魚血糖含量均呈現(xiàn)出先上升后下降的趨勢,最終SD1組幼魚血糖含量顯著低于SD2、SD3組(P0.05)。總膽固醇和總蛋白未發(fā)生顯著性變化。在實驗結束時,SD3組幼魚血液中甘油三酯含量顯著高于SD1、SD2組(P0.05),且SD1和SD2組之間無顯著性差異。綜合上述結果推斷俄羅斯鱘鱘幼魚能量利用順序是先血糖之后是血清蛋白,最后利用血脂。在密度達到12.12kg/m3時,皮質醇含量急劇增加,加速幼魚糖異生作用,耗能增加,俄羅斯鱘幼魚生長明顯受到抑制。3、高養(yǎng)殖密度組幼魚SOD活性顯著低于中低密度組(P0.05),表明較高的養(yǎng)殖密度打破機體抗氧化系統(tǒng)的平衡,抑制機體抗氧化功能的發(fā)揮,使機體清除自由基的能力’下降,體內過氧自由基含量升高,對機體造成損傷。同時實驗結果顯示SD3組幼魚MDA含量顯著高于SD1、SD2組(P0.05),這是脂質過氧化反應增強,脂質過氧化產物增多的表現(xiàn)。實驗結果可以看出,過分提高養(yǎng)殖密度,將會導致俄羅斯鱘幼魚出現(xiàn)擁擠脅迫,使機體的自由基“穩(wěn)態(tài)性動態(tài)平衡”遭受破壞,影響機體的正常生理狀態(tài)。SD3組俄羅斯鱘幼魚脾臟系數(shù)顯著低于SD1、SD2組水平(P0.05),血液溶菌酶活性以及C3補體因子含量也顯著降低,表明長期的擁擠脅迫致使高密度組俄羅斯鱘幼魚脾臟嚴重受損,免疫功能也受到一定程度的抑制,且隨養(yǎng)殖密度增加抑制效應愈加嚴重。
[Abstract]:In order to study the effects of different culture densities on the growth, blood physiology, biochemistry and non-specific immune function of juvenile Acipenser (Acipenser gueldenstaedti) in flowing water culture system. The juvenile Acipenser Russian Sturgeon with initial body weight of (29.70 鹵1.32) g was cultured in flowing water ponds (4.4 脳 4.4 脳 0.45 m3) with 3 densities of 2.5kg/m3 (SD1) 3.6 kg / m3 (SD2) and 4.7kg/m3 (SD3). The experiment period was 90 days. The results of the experiment are as follows: 1. The weight gain rate (WG), growth rate of body length (LG), specific growth rate (SGR) and feed conversion rate (FCR) in the low density group (SD1) were: 1: 362.01 + 15.87 鹵15.87 鹵5.79 鹵0.03114.95 鹵4.52, respectively, which were significantly higher than those in the high density group (SD3) (272.30 鹵2.74) 46.34 鹵6.221.53 鹵0.042 (94.49 鹵1.96) (P0.05), while in the SD3 group, the corresponding indexes were significantly higher than those in the high density group (SD3) (272.30 鹵2.74 鹵6.221.53 鹵0.049 鹵1.96) (P0.05). The degree and mortality of juvenile fish in SD1 group were significantly higher than those in SD1 group (P0.05). With the increase of culture density, the contents of protein and fat decreased, the content of water and ash increased, but there was no significant difference (P0.05). The results showed that the high culture density had a significant negative effect on the growth of juvenile Acipenser Russian Sturgeon, and the chronic stress of high culture density could inhibit the physiological and biochemical indexes of juvenile Acipenser Russian Sturgeon to some extent. There was a significant positive correlation between hemoglobin content and culture density. There was a significant difference in the white blood cell content of juvenile Acipenser Russian Sturgeon (SD3) compared with SD1 SD2 group (P0.05), which indicated that long-term high density culture had a certain degree of pathological damage to juvenile Acipenser Russian Sturgeon. The blood glucose content of juvenile fish in each density group increased first and then decreased, and the blood glucose content in SD1 group was significantly lower than that in SD2 + SD3 group (P0.05). Total cholesterol and total protein did not change significantly. At the end of the experiment, the content of triglyceride in the blood of young fish in SD3 group was significantly higher than that in SD1 / SD2 group (P0.05), and there was no significant difference between SD1 and SD2 groups. According to the above results, the order of energy utilization of juvenile Acipenser Russian Sturgeon is first blood sugar, then serum protein, and finally blood lipid. When the density reached 12.12kg/m3, the content of cortisol increased sharply, which accelerated the glycosylation of juvenile fish and increased the energy consumption. The growth of juvenile Acipenser Russian Sturgeon was inhibited significantly. The SOD activity of juvenile in high density group was significantly lower than that in medium and low density group (P0.05), which indicated that higher culture density upset the balance of antioxidant system and inhibited the exertion of antioxidant function. The ability of scavenging free radical is decreased, and the content of peroxy free radical is increased. The results showed that the MDA content of young fish in SD3 group was significantly higher than that in SD1 SD2 group (P0.05), which was the result of increased lipid peroxidation and increased lipid peroxidation products. The experimental results show that excessive increase in the density of culture will lead to crowding stress in juvenile Sturgeon and destroy the free radical "steady state dynamic balance" of the body. The spleen coefficient of juvenile Acipenser Russian Sturgeon in SD3 group was significantly lower than that in SD1 SD2 group (P0.05), and the activity of hemolytic enzyme and the content of C3 complement factor were also significantly decreased in SD3 group. The results showed that the spleen of juvenile Acipenser Russian Sturgeon in high density group was seriously damaged and the immune function was inhibited to a certain extent during the long period of crowded stress, and the inhibitory effect was more serious with the increase of culture density.
【學位授予單位】：中國海洋大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S965.215

【相似文獻】

相關期刊論文 前10條

1 夏華;李威名;陳阿琴;徐國成;戴習林;呂為群;;兩種養(yǎng)殖密度下鯽魚生長和免疫酶活性的比較[J];廣東農業(yè)科學;2013年12期

2 李志安;投喂浮性飼料 促進健康養(yǎng)魚[J];漁業(yè)致富指南;2002年18期

3 王桂芹;牛小天;李子平;蘆洪梅;朱興華;李清華;;飼料中添加維生素E對不同養(yǎng)殖密度鯉魚生產性能的影響[J];中國飼料;2010年02期

4 張曦文;吳垠;賀茹靖;蘇延明;劉琦;;循環(huán)水養(yǎng)殖模式下養(yǎng)殖密度對青石斑魚生長及生理指標的影響[J];大連海洋大學學報;2012年06期

5 ;在不同的氣候季節(jié)選擇恰當?shù)姆硼B(yǎng)密度[J];漁業(yè)現(xiàn)代化;2013年02期

6 李玉全;李健;王清印;張海艷;;養(yǎng)殖密度對工廠化對蝦養(yǎng)殖池氮磷收支的影響[J];中國水產科學;2007年06期

7 郭建誼;駱明飛;羅俊標;李勇;李望東;盤潤洪;;不同養(yǎng)殖密度對雜交尖塘鱧幼魚培育的影響[J];科學養(yǎng)魚;2014年03期

8 陳永勝,姚維志,謝大敬;不同養(yǎng)殖密度下鱘魚生長規(guī)律的研究[J];水利漁業(yè);2004年05期

9 廖銳;區(qū)又君;勾效偉;;養(yǎng)殖密度對魚類福利影響的研究進展Ⅰ．死亡率、生長、攝食以及應激反應[J];南方水產;2006年06期

10 蕭云樸;陳舜;伍德瀛;李定海;;養(yǎng)殖密度對蝦夷扇貝在浙江南麂海區(qū)生長的影響[J];南方水產;2009年05期

相關會議論文 前2條

1 白慶利;于洪賢;張玉勇;賈志英;尹家勝;包玉龍;李建興;;養(yǎng)殖密度對哲羅魚稚魚生長和存活的影響[A];2008年中國水產學會學術年會論文摘要集[C];2008年

2 吳增明;;肉雞至關重要的前十天[A];2008首屆中國白羽肉雞產業(yè)發(fā)展大會會刊[C];2008年

相關重要報紙文章 前10條

1 記者 高燕;牲畜養(yǎng)殖密度不宜過大[N];新疆科技報(漢);2009年

2 車烏丘;養(yǎng)殖密度過高導致扇貝死亡[N];中國漁業(yè)報;2012年

3 ;如何讓蚯蚓高產分期飼養(yǎng)[N];山西科技報;2003年

4 鄭衛(wèi)生;養(yǎng)龜要獲益 四項需注意[N];河南科技報;2012年

5 蔣麗英;大蝦為何越來越少[N];蘇州日報;2006年

6 姜陽;如何讓蚯蚓高產[N];農民日報;2002年

7 中養(yǎng)網;蚯蚓的高產措施[N];四川科技報;2006年

8 黎笠;養(yǎng)殖密度高羅非魚易發(fā)肥胖病[N];中國漁業(yè)報;2013年

9 曹靜;秋季養(yǎng)殖密度過高易引發(fā)泥鰍水腫與肝膽病[N];江蘇農業(yè)科技報;2012年

10 市水產處 管清雅;夏季魚病如何預防[N];渭南日報;2005年

相關博士學位論文 前1條

1 李賢;循環(huán)水系統(tǒng)中基于魚類福利學特征性指示因子的基礎研究[D];中國科學院研究生院（海洋研究所）;2012年

相關碩士學位論文 前9條

1 鄧超準;養(yǎng)殖密度和原花青素對星洲紅魚生長及生理生化的影響[D];集美大學;2015年

2 宋志飛;養(yǎng)殖密度對流水養(yǎng)殖系統(tǒng)中俄羅斯鱘幼魚生長、血液生理生化以及非特異性免疫的影響[D];中國海洋大學;2015年

3 董曉煜;溶解氧水平與養(yǎng)殖密度對褐牙鲆幼魚生長及生理機能的影響[D];中國海洋大學;2008年

4 丁厚猛;養(yǎng)殖密度對雜交幼魚鱘攝食、生長及生理機能的影響[D];中國海洋大學;2014年

5 趙霞;不同養(yǎng)殖密度對兩種鲆鰈魚類生長及免疫指標的影響[D];中國海洋大學;2010年

6 宋文華;養(yǎng)殖密度和溫度對草魚（Ctenopharyngodonidellus）生長和生理生化指標的影響[D];中國海洋大學;2012年

7 顏付云;循環(huán)海水養(yǎng)魚系統(tǒng)中氮磷收支的研究[D];寧波大學;2012年

8 劉文奎;烏鱧（♂）×斑鱧（♀）雜交F_1代生長與存活的研究[D];華中農業(yè)大學;2007年

9 曹陽;養(yǎng)殖密度和限食投飼對俄羅斯鱘幼魚生長和生理生化指標的影響[D];華東師范大學;2014年

，

本文編號：2090239