本文選題：稻瘟病菌 + MoVps41　； 參考：《福建農(nóng)林大學(xué)》2017年碩士論文

【摘要】：稻瘟病菌侵染以水稻為主的眾多重要農(nóng)作物,同時(shí)也是研究絲狀真菌的生長(zhǎng)、發(fā)育和發(fā)病機(jī)理的重要模式生物。內(nèi)吞途徑對(duì)稻瘟病菌致病性的影響逐漸被證明,在真核生物中,從真菌到植物和動(dòng)物,HOPS 復(fù)合體(Vps11、Vps16、Vps18、Vps33、Vps39 和 Vps41)對(duì)同型液泡融合、液泡生成和胞吐運(yùn)輸具有重要作用。然而,它在稻瘟病菌中的功能尚未被研究。本研究搜索到酵母Vps41在稻瘟病菌中的同源蛋白,并對(duì)該基因(MGG_03313.7)進(jìn)行敲除,實(shí)驗(yàn)結(jié)果證明它是稻瘟病菌液泡分選蛋白41(MoVps41),也是HOPS復(fù)合體的一個(gè)亞基。MoVps41蛋白的缺失導(dǎo)致稻瘟病菌的有性生殖和無性生殖受阻,生長(zhǎng)速率減慢,對(duì)水稻葉片、大麥葉片和水稻根部喪失了致病性;MoVps41蛋白缺失導(dǎo)致液泡碎片化,喪失了對(duì)胞內(nèi)過量金屬離子的解毒作用;胞外抑制劑測(cè)試試驗(yàn)表明MoVps41蛋白的缺失對(duì)細(xì)胞壁的敏感性、細(xì)胞膜的滲透壓和囊泡運(yùn)輸造成了嚴(yán)重的影響。
[Abstract]:Magnaporthe grisea infects many important crops, mainly rice, and is also an important model organism to study the growth, development and pathogenesis of filamentous fungi. The effects of endocytosis pathway on pathogenicity of rice blast fungus have been gradually demonstrated. In eukaryotes, the VPS11 / VPS16 / VPS3VPS39 and VPS41 play an important role in the syndromic vacuolar fusion, vacuolar formation and emetic transport from fungi to plants and animals. However, its function in rice blast fungus has not been studied. In this study, the homologous protein of yeast Vps41 in Magnaporthe oryzae was found, and the gene MGG03313.7) was knocked out. The results showed that it was a vacuolar sorting protein of Magnaporthe oryzae, and it was also a subunit of HOPS complex. The deletion of MoVPS41 protein resulted in the inhibition of sexual reproduction and asexual reproduction, and the decrease of growth rate in rice leaves. The loss of pathogenicity of MoVps41 protein in leaves and roots of barley resulted in vacuolar fragmentation and detoxification of intracellular excess metal ions, and the extracellular inhibitor test showed that the deletion of MoVps41 protein was sensitive to cell wall. Membrane osmotic pressure and vesicle transport have serious effects.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S435.111.41
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本文編號(hào)：1986639