本文選題：西伯利亞杏 + 種源��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本研究基于SCoT分子標(biāo)記利用23個(gè)引物對(duì)內(nèi)蒙古西伯利亞杏10個(gè)種源的278個(gè)家系進(jìn)行遺傳多樣性,研究探討內(nèi)蒙古地區(qū)山杏資源的收集及保護(hù),為內(nèi)蒙古西伯利亞杏的保存與利用提供有效依據(jù)。結(jié)果顯示:1.23個(gè)引物共擴(kuò)增出292個(gè)位點(diǎn),其中多態(tài)性位點(diǎn)(NPB)為289個(gè),平均每個(gè)引物擴(kuò)增的多態(tài)性位點(diǎn)為12.6條,SCoT21、SCoT32、SCoT53三個(gè)引物擴(kuò)增的位點(diǎn)最多為17條,多態(tài)性比率為100%;引物SCoT3擴(kuò)增的位點(diǎn)最少為9條,多態(tài)性比率為90%。2.內(nèi)蒙古西伯利亞杏種源引物多態(tài)性位點(diǎn)比率為97.94%,等位基因數(shù)(Na)為1.9803,有效等位基因數(shù)(Ne)為1.4252,Nei's遺傳多樣性指數(shù)(H)為0.2571,Shannon's信息多樣性指數(shù)(I)為0.3989;10個(gè)種源等位基因數(shù)范圍在1.3934～1.6230;有效等位基因數(shù)范圍在1.1956～1.3130;Nei's遺傳多樣性指數(shù)范圍0.1181～0.1871;Shannon's信息多樣性指數(shù)范圍在0.1812～0.2859;其中科右中旗種源的遺傳多樣性水平最高,其次是科左后旗;萬(wàn)家溝種源的遺傳多樣性最低。遺傳分化系數(shù)(Gst)為0.3601,基因流(Nm)為0.8884。經(jīng)分子方差分析39%的遺傳變異來(lái)自種源間,61%的遺傳變異來(lái)自種源內(nèi)。3.內(nèi)蒙古西伯利亞杏種源遺傳相似度范圍0.7799～0.9694,遺傳距離范圍在0.0311～0.2486:基于遺傳相似度的聚類分和遺傳距離的主坐標(biāo)分析均將供試10個(gè)種源分成2組,大部分地理距離相近的種源聚類到一起。研究表明,內(nèi)蒙古西伯利亞杏有較高的遺傳多樣性。
[Abstract]:In this study, the genetic diversity of 278 families from 10 provenances of Siberian apricot in Inner Mongolia was studied by using 23 primers based on SCoT molecular markers, and the collection and protection of apricot resources in Inner Mongolia were studied. It provides an effective basis for the preservation and utilization of Siberian apricot in Inner Mongolia. The results showed that 292 loci were amplified by 23 primers, of which 289 were polymorphic loci (NPB), and the average number of polymorphic loci per primer amplified was 12.6 SCoT21 / SCoT32SCoT53. The polymorphic ratio was 100 and the primer SCoT3 amplified the least 9 loci and the polymorphic ratio was 90. 2. 2. In Inner Mongolia, the ratio of primer polymorphic loci was 97.94, the number of alleles was 1.9803, the effective number of alleles was 1.4252Neis genetic diversity index (HH) was 0.2571Shannon 's information diversity index (I) was 0.3989.The allele number of 10 provenances was in the range of 1.9803, and the number of alleles in 10 provenances was in the range of 1.9803. The number of effective alleles was in the range of 1.19561.3130Neis genetic diversity index 0.1181 / 0.1871 Shannons information diversity index was 0.18120.2859.The genetic diversity level of the middle flag provenance was the highest. The genetic diversity of Wanjiagou provenance was the lowest. The coefficient of genetic differentiation was 0.3601, and the coefficient of genetic differentiation was 0.8884. By molecular variance analysis, 39% of the genetic variation came from the interprovenance. 61% of the genetic variation came from within the provenance. In Inner Mongolia, the genetic similarity of Siberian apricot provenances was 0.7799 / 0.9694, and the genetic distance was 0.03110.2486. The cluster and the principal coordinate analysis of genetic distance based on genetic similarity divided 10 provenances into two groups. Most provenances with similar geographical distances are clustered together. The results showed that there was high genetic diversity in Siberian apricot in Inner Mongolia.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S662.2

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本文編號(hào)：1956445