發(fā)布時間：2018-05-29 12:02

  本文選題：馬鈴薯 + StRac��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：小G蛋白是一類單體GTP結(jié)合蛋白,具有GTPase的活性。Rac是小G蛋白家族中的重要成員,已有研究結(jié)果表明小G蛋白Rac能夠參與多種細(xì)胞信號調(diào)控過程,其中包括植物抗病防衛(wèi)反應(yīng)、花粉管的伸長、細(xì)胞分化、根毛的發(fā)育以及細(xì)胞骨架的重組等。本研究以.StRac轉(zhuǎn)基因馬鈴薯植株為材料,測定StRac轉(zhuǎn)基因植株的各項(xiàng)生理指標(biāo)和廣譜抗性水平,具體結(jié)果如下:1.馬鈴薯StRac轉(zhuǎn)基因植株試管苗各項(xiàng)生理指標(biāo)的測定結(jié)果表明,過量表達(dá)激活型CA-StRac基因植株的根/莖/葉的鮮重、干重、單株株高、莖粗、葉片數(shù)、葉面積、節(jié)長、節(jié)數(shù)、根系長度、根條數(shù)均比空載體P1300-GFP的高,分別增加了 33.33%、11.54%、34.44%、12.99%、25.50%、28.89%、22.83%、10%、2.30%、6.39%、46.10%、7.8%、25%、27.2%。相反,過量表達(dá)失活型DN-StRac基因植株的各項(xiàng)生理指標(biāo)除莖粗比空載體P1300-GFP增加6.25%外,其余各項(xiàng)指標(biāo)比空載體P1300-GFP有所降低,降低比率分別為 64.82%、42.31%、46.67%、63.28%、35.30%、40%、8.7%、8.4%、39%、4.7%、1.9%、35.42%、10.61%。2.馬鈴薯StRac轉(zhuǎn)基因植株大田中各項(xiàng)生理指標(biāo)測定的結(jié)果表明,過量表達(dá)激活型CA-StRac基因植株的花期比空載體P1300-GFP僅僅提早一天。單個薯塊的大小、重量、芽眼數(shù)、分枝數(shù)分別比空載體P1300-GFP增加了 22.12%、49.82%、2.71%、10.41%。3.馬鈴薯StRac轉(zhuǎn)基因植株廣譜抗性鑒定結(jié)果表明,接種早疫病菌、黑痣病菌、枯萎病菌和黃萎病菌后,過量表達(dá)CA-StRac的轉(zhuǎn)基因馬鈴薯抗性均有所提高;其中接種早疫病菌后轉(zhuǎn)基因馬鈴薯葉片上病斑面積相比對照降低了 72.8%;在薯片上接種黑痣病菌后,病斑面積也相應(yīng)降低了 35.6%;接種枯萎病菌和黃萎病菌后,病情指數(shù)相比對照也相應(yīng)降低了 40%和11.6%。說明StRac能夠正向調(diào)控馬鈴薯廣譜抗性的建立。
[Abstract]:Small G protein is a kind of monomeric GTP binding protein, which has the activity of GTPase. Racc is an important member of small G protein family. It has been shown that small G protein Rac can participate in many cell signal regulation processes, including plant disease resistance and defense response. Pollen tube elongation, cell differentiation, root hair development and cytoskeleton recombination. In this study, the physiological indexes and broad-spectrum resistance of StRac transgenic potato plants were determined. The results were as follows: 1. The physiological indexes of potato StRac transgenic plantlets in vitro were determined. The results showed that the fresh weight, dry weight, plant height, stem diameter, leaf number, leaf area, node length and node number of over-expressed CA-StRac gene plants were measured. The length of root system and the number of root strips were higher than that of empty carrier P1300-GFP, and they were increased by 33.333.11.54 and 12.99, and 25.50 and 28.89, respectively, and there was a score of 22.83and a score of 22.833.It was about 2.306.396.390.46.100.7.8250.27.20.The root length and the number of roots were higher than those of the empty carrier P1300-GFP, and the number of roots was increased by 33.333.11.54 and 11.54, respectively. On the contrary, with the exception of 6.25% increase in physiological index of inactivated DN-StRac gene plant, the other physiological indexes of inactivated DN-StRac gene plant were lower than that of empty vector P1300-GFP by 6.25%, and the other indexes were decreased by 64.82% (64.82%) than empty vector P1300-GFP (64.82%) by the ratio of 63.2835.308.78.78.43.4394.77.The other indexes were lower than that of empty vector P1300-GFP (64.82%). The physiological indexes of potato StRac transgenic plants were determined in the field. The results showed that the flowering time of the plants with overexpression of activated CA-StRac gene was only one day earlier than that of empty vector P1300-GFP. Compared with empty carrier P1300-GFP, the size, weight, bud eye number and branching number of a single tuber increased 22.12% and 10.41% than that of empty carrier P1300-GFP. The results of broad-spectrum resistance identification of potato StRac transgenic plants showed that after inoculation with early blight, nevus, Fusarium wilt and Verticillium wilt, the resistance of transgenic potato with overexpression of CA-StRac was increased. After inoculation with Phytophthora infestans, the leaf spot area of transgenic potato decreased by 72.8 compared with the control. After inoculating potato chips with nevus, the spot area was also reduced by 35.60.After inoculation with Fusarium wilt and Verticillium wilt, The disease index was also decreased by 40% and 11. 6% compared with the control. The results showed that StRac could positively regulate the establishment of broad-spectrum resistance in potato.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S532

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