發(fā)布時(shí)間：2018-05-28 07:49

  本文選題：甘藍(lán)型油菜 + 廣適性��； 參考：《華中農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：光周期對(duì)植物的生長(zhǎng)發(fā)育有著重要的影響,油菜是長(zhǎng)日照植物,尋找具有廣適應(yīng)性的油菜品種,有利于甘藍(lán)型油菜的種質(zhì)資源的創(chuàng)新,提高甘藍(lán)型油菜對(duì)不同播種季節(jié)和地理緯度的適應(yīng)性,對(duì)油菜的遺傳改良和品種選育有著重要的意義。本研究以對(duì)光周期敏感的丙409和對(duì)光周期不敏感的中雙8號(hào)為親本構(gòu)建的包含有150個(gè)株系的DH群體為材料,利用DH群體的遺傳連鎖圖譜,采用復(fù)合區(qū)間作圖法,通過(guò)3年多點(diǎn)的不同光周期試驗(yàn),對(duì)開(kāi)花時(shí)間進(jìn)行QTL定位和效應(yīng)分析。同時(shí)在氣候箱里對(duì)親本進(jìn)行不同的光周期處理(16h/8h,12h/12h),長(zhǎng)到一個(gè)月時(shí)從光照開(kāi)始的時(shí)間開(kāi)始取樣,每隔4個(gè)小時(shí)取一次樣,取樣時(shí)間為0h(光照開(kāi)始時(shí)),4h,8h,12h,16h,20h,24h。取葉片提RNA,兩個(gè)重復(fù),進(jìn)行RNA-seq,找到控制花期的差異基因。主要研究結(jié)果如下:1.DH群體在6個(gè)環(huán)境下的開(kāi)花期QTL信息DH群體在4個(gè)地點(diǎn)(武漢,和政,烏魯木齊,韶關(guān))共6個(gè)環(huán)境(3年*1點(diǎn)+1年*3點(diǎn))下調(diào)查開(kāi)花期,共檢測(cè)到11個(gè)與初花期有關(guān)的QTL,其中在6個(gè)環(huán)境中都檢測(cè)到了C8染色體上的同一個(gè)QTL,平均解釋了表型變異的11.31%;在E1,E2,E3,E6環(huán)境下都檢測(cè)到在A(yíng)3染色體上的同一個(gè)QTL,平均解釋了表型變異的7.89%;在E1,E3,E5,E6環(huán)境下都檢測(cè)到了在A(yíng)2染色體同一個(gè)QTL,解釋了表型變異的10.32%。只在一個(gè)地點(diǎn)檢測(cè)到QTL有7個(gè)。2.光周期敏感性的分析對(duì)不同地點(diǎn)的開(kāi)花期做了光周期敏感性分析,共檢測(cè)到11個(gè)和光周期敏感性相關(guān)的位點(diǎn)。其中在E1-E6,E2-E6,E3-E6,E3-E4,E4-E6,E5-E6都檢測(cè)到了在A(yíng)2染色體上的同一個(gè)QTL位點(diǎn),平均解釋了表型變異的12.59%;在E1-E6,E2-E5,E2-E6,E3-E6,E4-E5,E4-E6都檢測(cè)到了在C8染色體上的同一個(gè)QTL位點(diǎn),平均解釋表型變異的17%;在E1-E6,E2-E4,E2-E6環(huán)境下都檢測(cè)到了A3染色體上的QTL位點(diǎn),平均解釋了表型變異的5.12%。只在兩個(gè)環(huán)境比對(duì)下檢測(cè)到的QTL有5個(gè)。3.甘藍(lán)型油菜在不同光周期下的轉(zhuǎn)錄組分析在相同光周期下的相同時(shí)間點(diǎn)對(duì)丙409和中雙8號(hào)的相對(duì)表達(dá)進(jìn)行分析,發(fā)現(xiàn)16h光周期條件下24h時(shí)的差異表達(dá)基因最多,為19965個(gè);在12個(gè)小時(shí)光照下T12差異表達(dá)基因最多,為18859個(gè)。比較了丙409和中雙8號(hào)分別在16h與12h光周期相比在相同時(shí)間點(diǎn)DEG的表達(dá)情況,發(fā)現(xiàn)丙409在24h的時(shí)候差異表達(dá)基因最多,為20396個(gè);中雙8號(hào)在16h時(shí)差異表達(dá)基因最多為20237個(gè)。在光周期的臨界點(diǎn)基因上調(diào)下調(diào)表達(dá)比較多。4.候選基因的分析QTL結(jié)果結(jié)合轉(zhuǎn)錄組數(shù)據(jù)找到A2,A3,C8染色體上在區(qū)段內(nèi)的差異表達(dá)基因,有676個(gè)。沒(méi)有發(fā)現(xiàn)和開(kāi)花相關(guān)的基因,這些基因在KEGG分析中,淀粉和蔗糖代謝,嘌呤的代謝所包含的基因較多。
[Abstract]:Photoperiod has an important effect on the growth and development of plants. Rape is a long sunshine plant. It is beneficial to the innovation of germplasm resources of Brassica napus to search for a variety of rapeseed with wide adaptability. It is of great significance to improve the adaptability of Brassica napus to different sowing seasons and geographical latitudes, and to improve the genetics and breeding of Brassica napus. In this study, a DH population containing 150 lines was constructed from Pro409, which was sensitive to photoperiod, and Zhongshuang 8, which was insensitive to photoperiod. The genetic linkage map of DH population was used to make use of compound interval mapping method. QTL location and effect analysis of flowering time were carried out through different photoperiod experiments of more than 3 years. At the same time, the parents were treated with different photoperiod at 16 h / 8 h / 12 h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h / 12h, respectively in the climate box. The RNAs were extracted from the leaves and two repeats were carried out to find the differentially expressed genes controlling the flowering period. The main results were as follows: 1. The QTL information of DH population in 6 environments was investigated in 4 locations (Wuhan, Hezheng, Urumqi, Shaoguan) in 6 environments (3 points at 1: 1 in 3 years), and the flowering period of DH population was investigated in 4 locations (Wuhan, Hezheng, Urumqi, Shaoguan). A total of 11 QTLs related to early flowering were detected, of which the same QTLs on the C8 chromosome were detected in 6 environments, which accounted for the average phenotypic variation of 11.31 cells, and the same QTLs on the A3 chromosome were detected in the E1E2E3E6 environment. 7.89% of phenotypic variation was explained, and the same QTL on A2 chromosome was detected in E1E3 and E5 / E6 environment, which explained 10.32% of phenotypic variation. There are only 7. 2. 2 QTL detected in one site. The photoperiod sensitivity analysis was carried out for the flowering period of different locations, and a total of 11 sites related to photoperiod sensitivity were detected. Among them, the same QTL locus on the A2 chromosome was detected in E1-E6E2-E6-E6-E6-E3-E4E4-E6-E5-E6, and the same QTL locus on the C8 chromosome was detected in the E1-E6E2-E2-E5 E2-E2-E6-E6E3-E6E4-E5E4-E6 locus on the chromosomes A2, and in the E1-E6E6E2-E5-E5, E1-E6E6E2-E2-E2-E6E3-E6E4-E5E4-E6, respectively. The QTL loci on the A3 chromosome were detected in E1-E6 E2-E4E2-E6 environment, and the average explained phenotypic variation was 5.12%. There were only 5. 3. 3 QTL detected in two environments. Transcriptome analysis of Brassica napus under different photoperiod at the same time point under the same photoperiod, the relative expressions of Pro409 and Zhongshuang 8 were analyzed. The results showed that the most differentially expressed genes were at 16 h photoperiod for 24 h (19965). The number of differentially expressed genes was 18859 under 12 hours of illumination. The expression of DEG at 16 h and 12 h photoperiod was compared between P409 and Zhongshuang 8, respectively. It was found that the highest number of differentially expressed genes was 20396 at 24h, and 20237 at 16h in Zhongshuang8. At the critical point of photoperiod, the up-regulation and down-regulation of genes are more. 4. The results of QTL analysis and transcriptome data showed that there were 676 differentially expressed genes in the region of A2A3C8 chromosome. No genes related to flowering were found. In KEGG analysis, the metabolism of starch, sucrose and purine contained more genes.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S565.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羅玉秀;張生萍;許唱唱;馬小崗;杜德志;;特早熟春性甘藍(lán)型油菜sBnFLD基因的克隆及表達(dá)[J];西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版);2016年11期

2 黃吉祥;熊化鑫;潘兵;倪西源;張曉玉;趙堅(jiān)義;;油菜開(kāi)花期QTL定位及與粒重的遺傳關(guān)聯(lián)性[J];中國(guó)農(nóng)業(yè)科學(xué);2016年16期

3 關(guān)周博;田建華;董育紅;;我國(guó)油菜發(fā)展的現(xiàn)狀、面臨的問(wèn)題以及應(yīng)對(duì)策略[J];陜西農(nóng)業(yè)科學(xué);2016年03期

4 羅玉秀;羅春燕;;春性甘藍(lán)型油菜開(kāi)花時(shí)間的遺傳分析[J];北方園藝;2015年19期

5 周慶紅;曾勇軍;黃英金;;油菜開(kāi)花期QTL圖譜整合及開(kāi)花候選基因挖掘[J];核農(nóng)學(xué)報(bào);2014年04期

6 祁云霞;劉永斌;榮威恒;;轉(zhuǎn)錄組研究新技術(shù):RNA-Seq及其應(yīng)用[J];遺傳;2011年11期

7 沈金雄;傅廷棟;;我國(guó)油菜生產(chǎn)、改良與食用油供給安全[J];中國(guó)農(nóng)業(yè)科技導(dǎo)報(bào);2011年01期

8 吳連成;常麗麗;陳曉;庫(kù)麗霞;;CO基因的調(diào)控表達(dá)與植物光周期反應(yīng)[J];中國(guó)農(nóng)學(xué)通報(bào);2010年02期

9 姜妍;冷建田;費(fèi)志宏;馮濤;祖?zhèn)?王連錚;韓天富;吳存祥;;廣適應(yīng)大豆品種中黃13的光周期反應(yīng)[J];大豆科學(xué);2009年03期

10 蔡長(zhǎng)春;陳寶元;傅廷棟;涂金星;;甘藍(lán)型油菜開(kāi)花期和光周期敏感性的遺傳分析[J];作物學(xué)報(bào);2007年02期

相關(guān)會(huì)議論文 前2條

1 李保軍;王灝;王曉東;趙衛(wèi)國(guó);趙亞軍;田建華;栗茂騰;;甘藍(lán)型油菜開(kāi)花性狀QTL分析[A];中國(guó)作物學(xué)會(huì)——2015年學(xué)術(shù)年會(huì)論文摘要集[C];2015年

2 傅廷棟;周永明;;油菜遺傳改良與生物柴油[A];中國(guó)作物學(xué)會(huì)2006年學(xué)術(shù)年會(huì)論文集[C];2006年

相關(guān)博士學(xué)位論文 前7條

1 杜春芳;甘藍(lán)型油菜低溫誘導(dǎo)的轉(zhuǎn)錄組和蛋白組分析[D];華中農(nóng)業(yè)大學(xué);2016年

2 杜軼威;水稻開(kāi)花相關(guān)RING蛋白1(FRRP1)基因的克隆和開(kāi)花功能分析[D];中國(guó)農(nóng)業(yè)大學(xué);2016年

3 譚俊杰;水稻CONSTANS-like基因OsCOL10作用于光周期開(kāi)花途徑的分子遺傳與生化分析[D];湖南大學(xué);2015年

4 李浩杰;蕓薹屬基因組中開(kāi)花時(shí)間基因的鑒定和進(jìn)化分析[D];四川農(nóng)業(yè)大學(xué);2013年

5 趙榮秋;甘藍(lán)春化相關(guān)基因BoVIN3的克隆及分析[D];東北農(nóng)業(yè)大學(xué);2012年

6 蔡長(zhǎng)春;甘藍(lán)型油菜開(kāi)花時(shí)間和光周期敏感性的遺傳分析和QTL定位[D];華中農(nóng)業(yè)大學(xué);2006年

7 梅德圣;甘藍(lán)型油菜株高和開(kāi)花時(shí)間的QTL定位及黃籽性狀的分子標(biāo)記[D];中國(guó)農(nóng)業(yè)科學(xué)院;2005年

相關(guān)碩士學(xué)位論文 前10條

1 蔡靜;甘藍(lán)型油菜一個(gè)主效開(kāi)花期QTL_qFT_(c2-1)的精細(xì)定位[D];華中農(nóng)業(yè)大學(xué);2016年

2 熊化鑫;油菜開(kāi)花期QTL分析以及三個(gè)主效QTL的精細(xì)定位[D];浙江師范大學(xué);2016年

3 王曉輝;源于不同生態(tài)區(qū)春小麥品種“阿勃”的廣適性研究[D];青海大學(xué);2014年

4 周曉晨;甘藍(lán)型油菜早熟相關(guān)性狀QTL定位及開(kāi)花基因BnGI的克隆[D];上海交通大學(xué);2014年

5 石鵬;TN DH群體高密度遺傳圖譜構(gòu)建及其控制重要農(nóng)藝性狀QTL的再定位[D];華中農(nóng)業(yè)大學(xué);2013年

6 鄭本川;甘藍(lán)型油菜開(kāi)花調(diào)控轉(zhuǎn)錄因子CONSTANS同源基因的克隆及其定量表達(dá)分析[D];四川農(nóng)業(yè)大學(xué);2013年

7 羅潔;甘藍(lán)型油菜及近緣親本春化基因VIN3的克隆與進(jìn)化分析[D];華中科技大學(xué);2013年

8 洪寶華;利用重疊導(dǎo)入系定位油菜開(kāi)花期相關(guān)基因[D];華中農(nóng)業(yè)大學(xué);2012年

9 葉金英;FT、FD與SOC1對(duì)AP1基因表達(dá)調(diào)控的研究[D];廈門(mén)大學(xué);2009年

10 廖星;論提升我國(guó)油菜產(chǎn)業(yè)的發(fā)展戰(zhàn)略[D];華中農(nóng)業(yè)大學(xué);2004年

，

本文編號(hào)：1945899