本文選題：黃芩愈傷組織 + 水楊酸��； 參考：《吉林農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：黃芩(Scutellaria baicalensis Georgi)為唇形科多年生草本植物,根入藥,藥用價(jià)值高,市場(chǎng)需求量大,野生資源幾近枯竭,而栽培黃芩受遺傳和環(huán)境因素影響,質(zhì)量差異較大,不易人為控制。通過組織培養(yǎng)技術(shù)可以消除由遺傳差異帶來的影響,同時(shí)具有生長周期短,不受外界環(huán)境條件限制等優(yōu)勢(shì),已成為諸多藥用植物獲取天然藥用成分的途徑之一。在眾多提高藥效成分的方法中,外源添加誘導(dǎo)子已成為最常用且最有效的方法之一。SA和MeJA作為植物體內(nèi)重要的生物信息素物質(zhì),在植物信號(hào)轉(zhuǎn)導(dǎo)和抗逆反應(yīng)中發(fā)揮著重要作用,也是目前藥用植物應(yīng)用最多的非生物誘導(dǎo)子。本研究以黃芩愈傷組織為材料,通過添加不同種類濃度的誘導(dǎo)子,研究愈傷組織的生物量及其黃芩苷等藥效成分的含量,同時(shí)對(duì)黃芩苷合成途徑關(guān)鍵酶PAL、C4H、4CL和CHS活性及其抗氧化酶SOD、POD和CAT活性進(jìn)行測(cè)定,以期從代謝途徑關(guān)鍵酶和抗氧化酶系統(tǒng)兩個(gè)方面初步探究誘導(dǎo)子對(duì)黃芩愈傷組織次生代謝物積累的機(jī)制。主要研究結(jié)果如下:1、篩選不同外植體和不同濃度植物激素配比確定黃芩愈傷組織培養(yǎng)的最佳外植體為其帶節(jié)莖段,最佳培養(yǎng)條件為:MS+1.0mg/L 6-BA+1.5mg/L NAA,pH 5.8,培養(yǎng)溫度(25±1)℃,暗培養(yǎng)。黃芩愈傷組織生長周期為25天。2、不同濃度SA和MeJA單獨(dú)處理,愈傷組織鮮重生長受到抑制;SA濃度為10μmol/L、50μmol/L,MeJA濃度為10μmol/L、50μmol/L、100μmol/L時(shí)干重略高于對(duì)照,但差異均不顯著。SA和MeJA復(fù)配作用,愈傷組織鮮重生長得到促進(jìn),干重略低于對(duì)照,差異不顯著。3、SA單獨(dú)處理濃度為50μmol/L時(shí)黃芩苷含量積累最多,是對(duì)照的1.63倍,與對(duì)照差異顯著;MeJA濃度為50μmol/L時(shí)黃芩苷含量最大,是對(duì)照的1.83倍,與對(duì)照差異顯著;SA和MeJA復(fù)配作用,A9組(SA 100μmol/L+MeJA 100μmol/L)愈傷組織黃芩苷含量最高,是對(duì)照的1.10倍,與對(duì)照差異不顯著。4、SA濃度為10μmol/L時(shí)SOD活性最大,POD活性略低于對(duì)照,50μmol/L時(shí)CAT活性最大;MeJA濃度為50μmol/L時(shí)POD、CAT活性最大;SA和MeJA復(fù)配作用,A4組SOD活性最大,A9組POD活性最大,A7組CAT活性最大。5、SA濃度為10μmol/L時(shí)C4H活性最大,PAL活性在50μmol/L時(shí)略低于對(duì)照,4CL活性在50μmol/L時(shí)達(dá)到最大;MeJA濃度為200μmol/L時(shí)PAL活性達(dá)到最大,50μmol/L濃度時(shí)C4H活性最大,10μmol/L濃度4CL活性最大,100μmol/L濃度CHS活性最大;SA和MeJA復(fù)配作用整體上提高了PAL、C4H、4CL、CHS活性,其中A9組PAL活性最大,A6組CHS活性最大,A7組4CL活性最大。6、SA單獨(dú)處理黃芩苷含量與POD顯著負(fù)相關(guān),與CAT顯著正相關(guān);MeJA單獨(dú)處理黃芩苷含量與CAT呈顯著正相關(guān);SA和Me JA復(fù)配作用,黃芩苷含量與4CL呈極顯著正相關(guān)。
[Abstract]:Scutellaria baicalensis Georgii is a perennial herb of Labiatae with high medicinal value, high market demand and close depletion of wild resources. However, the quality of cultivated Scutellaria baicalensis is influenced by genetic and environmental factors, and the quality of cultivated Scutellaria baicalensis is not easy to be controlled artificially. The technique of tissue culture can eliminate the influence of genetic difference and has the advantages of short growth cycle and no restriction of external environment. It has become one of the ways for many medicinal plants to obtain natural medicinal components. Among the many methods to improve the pharmacodynamic components, exogenous inducers have become one of the most common and effective methods. SA and MeJA are important biological pheromones in plants and play an important role in plant signal transduction and stress resistance. It is also the most widely used abiotic elicitor in medicinal plants. In this study, the callus of Scutellaria baicalensis was used as material, and the biomass of callus and the content of baicalin and other effective components were studied by adding different kinds of inducers. At the same time, the activities of PALC4Hn4CL and CHS, and the activities of antioxidant enzymes SOD- POD and CAT, were also determined, which were the key enzymes of baicalin synthesis pathway. In order to explore the mechanism of secondary metabolites accumulation of Scutellaria baicalensis callus from the two aspects of key enzymes and antioxidant oxidase system of metabolic pathway. The main results were as follows: 1. The best explants with stem segments were determined by screening different explants and different concentrations of plant hormones. The optimum culture conditions were as follows: ph 5.8 of 1.0mg/L 6-BA 1.5mg/L NAA, 25 鹵1 鈩，

本文編號(hào)：1929973