發(fā)布時間：2018-05-19 11:09

  本文選題：酶制劑 + 配方篩選��； 參考：《沈陽農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本試驗通過體外消化試驗篩選出纖維素酶、木聚糖酶和葡萄糖氧化酶的最適配方,將篩選出的復(fù)合酶制劑配方應(yīng)用在蛋雞低能日糧中,研究復(fù)合酶制劑對蛋雞生產(chǎn)性能,蛋品質(zhì)、養(yǎng)分代謝率和血液指標(biāo)的影響。試驗結(jié)果如下:試驗一:復(fù)合酶配方的體外篩選1.本試驗采用國標(biāo)方法對3種酶的活性進(jìn)行測定,3種酶活性測定值為:木聚糖酶46863.47u/g,纖維素酶 8452.61u/g,葡萄糖氧化酶 1000.00u/g。2.本試驗采用3×3完全交叉設(shè)計試驗,使用體外消化法模擬飼料在機(jī)體內(nèi)消化情況,獲得消化產(chǎn)物,測定干物質(zhì)消化率。3種酶對干物質(zhì)消化率都具有極顯著作用,對干物質(zhì)消化率效果最佳的單酶劑量為:木聚糖酶4500u/g、纖維素酶250u/g、葡萄糖氧化酶300u/g。綜上所述,篩選的復(fù)合酶配方為:纖維素酶250u/g,木聚糖酶4500u/g,葡萄糖氧化酶300u/g。試驗二:低能日糧中添加不同水平的復(fù)合酶制劑對產(chǎn)蛋雞的生產(chǎn)性能、蛋品質(zhì)、養(yǎng)代謝率和血液指標(biāo)的影響。選擇480只蛋雞,隨機(jī)分為5組,每組3個重復(fù),每個重復(fù)32只雞。其中正對照組飼喂普通的基礎(chǔ)日糧,負(fù)對照組和試驗組的日糧比普通的基礎(chǔ)日糧降低能量0.21MJ/kg,使得試驗組的復(fù)合酶添加量的分別為200g/t、300g/t、400g/t。通過飼養(yǎng)試驗驗證此復(fù)合酶對蛋雞生產(chǎn)性能、蛋品質(zhì)、養(yǎng)分代謝率及血液指標(biāo)的影響,并判斷添加的最適劑量,為復(fù)合酶在蛋雞上的應(yīng)用提供依據(jù)。1.生產(chǎn)性能方面:降低能量對蛋雞的生產(chǎn)性能產(chǎn)生影響,添加復(fù)合酶制劑蛋雞生產(chǎn)性能具有改善作用。負(fù)對照組的平均蛋重比正對照組降低了 1.22%(P0.05),試驗1組的平均蛋重比正對照組升高了 1.38%(P0.01),比負(fù)對照組升高了 2.63%(P0.05),并且試驗1組的平均蛋重也顯著高于試驗2組和試驗3組(P0.05)。負(fù)對照組的產(chǎn)蛋率比正對照組降低了 10.01%(P0.01),試驗2組產(chǎn)蛋率比正對照組降低了 3.24%(P0.05),比負(fù)對照組升高了 7.53%(P0.01)。負(fù)對照組的料蛋比比正對照組降低了 16.11%(P0.05),試驗1組、試驗2組、試驗3組的料蛋比均低于負(fù)對照組,但是差異不顯著(P0.05)。所以在蛋雞生產(chǎn)性能方面,最優(yōu)試驗組為試驗2組。2.蛋品質(zhì)方面:試驗1組、試驗2組、試驗3組與正對照組和負(fù)對照組相比,蛋殼厚度、強(qiáng)度、蛋重、蛋白高度、哈夫單位以及蛋黃顏色均差異不顯著(P0.05)。3.養(yǎng)分代謝率方面:低能日糧中添加復(fù)合酶制劑具有促進(jìn)干物質(zhì)代謝率的作用,其中試驗3組的干物質(zhì)代謝率顯著高于負(fù)對照組,提高了 8.76%(P0.05),并且與正對照組之間差異不顯著(P0.05)。添加復(fù)合酶制劑促進(jìn)了粗蛋白代謝,試驗2組的粗蛋白代謝率比正對照組升高了 5.77%(P0.05),試驗3組粗蛋白代謝率比正對照組升高了10.60%(P0.01),試驗2組和試驗3組的粗蛋白代謝率比負(fù)對照組分別升高了 6.92%(P0.05)、11.80%(P0.01)。低能日糧中添加酶制劑能促進(jìn)飼料表觀代謝能,其中試驗1組、試驗3組表觀代謝能比負(fù)對照組分別升高了 8.73%、9.04%,差異顯著(P0.05),但是試驗1組與試驗3組均極顯著低于正對照組(P0.01)。因此在養(yǎng)分代謝率中效果較好的試驗組為試驗3組。4.血液指標(biāo)方面:試驗1組、試驗3組血清白蛋白較正對照組得到極顯著的升高(P0.01),并且極顯著地高于負(fù)對照組(P0.01);試驗2組、試驗3組血清球蛋白較負(fù)對照組得到了顯著地提高(P0.05),并且極顯著地高于正對照組;試驗1組血清中總蛋白含量較負(fù)對照組得到了顯著地提高(P0.05),試驗2組、試驗3組血清總蛋白含量極顯著地高于負(fù)對照組(P0.01),并且極顯著地高于正對照組(P0.01);試驗2組和試驗3組血清總膽固醇含量顯著高于正對照組和負(fù)對照組(P0.05);試驗1組血清甘油三酯較負(fù)對照組顯著性地降低(P0.05),并且極顯著地低于正對照組(P0.01),試驗2組、試驗3組血清甘油三酯與負(fù)對照組相比,差異不顯著(P0.05),但是極顯著地低于正對照組(P0.01)。酶制劑對血清中鈣的含量影響不大,各組之間差異不顯著(P0.05),試驗1組磷含量顯著高于正對照組和負(fù)對照組(P0.05),試驗3組磷含量極顯著地高于正對照組和負(fù)對照組(P0.01)。綜上所述,添加一定量的酶制劑能夠彌補(bǔ)降低能量對蛋雞產(chǎn)生的負(fù)面影響,能提高蛋雞生產(chǎn)性能、提高養(yǎng)分代謝率,促進(jìn)血液指標(biāo)。整體比較來看,200g/t蛋雞生產(chǎn)效果較好。
[Abstract]:In this experiment, the optimum formula of cellulase, xylanase and glucose oxidase was screened out through in vitro digestion test. The compound enzyme formulation was used in the low energy diet of laying hens, and the effects of compound enzyme preparation on the production performance, egg quality, nutrient metabolism rate and blood liquid index of laying hens were studied. The experimental results were as follows: Test 1: compound In vitro screening of enzyme formula in 1. experiments, the activity of 3 enzymes was measured by national standard method. The determination of the activity of the 3 enzymes was: xylanase 46863.47u/g, cellulase 8452.61u/g, and glucose oxidase 1000.00u/g.2. in this experiment, using 3 x 3 complete cross design test, using in vitro digestion method to simulate the digestion of feed in the body and obtain the results. The digestibility of the digestibility of dry matter has a very significant effect on the digestibility of dry matter. The optimum single enzyme dose for the digestibility of dry matter is: xylanase 4500u/g, cellulase 250u/g, and glucose oxidase 300u/g.. The selected compound enzyme is as follows: cellulase 250u/g, xylanase 4500u/g, glucose oxygen. Enzyme 300u/g. test two: the effects of different levels of compound enzymes on the production performance, egg quality, metabolism rate and blood indexes of laying hens were added to low energy diets. 480 layers were randomly divided into 5 groups, 3 repetitions in each group, and 32 chickens for each repetition. The normal control group was fed with the normal basal diet, the negative control group and the test group. The ratio of the compound enzyme added to the test group was 200g/t, 300g/t, and 400g/t., respectively, to verify the effect of the compound enzyme on the production performance, egg quality, nutrient metabolism rate and blood index, and to judge the optimum dosage of the compound enzyme, which provided the basis for the application of the compound enzyme in the laying hens. According to the production performance of.1., the production performance of laying hens was reduced by reducing energy. The average egg weight of the negative control group was 1.22% lower than that of the control group (P0.05). The average egg weight of the negative control group was 1.38% (P0.01) higher than that in the control group, and 2.63% (P0.05) was higher than that of the negative control group. The average egg weight of the 1 groups was also significantly higher than that of the test 2 and the 3 groups (P0.05). The egg production rate in the negative control group was 10.01% (P0.01) lower than that in the control group, and the rate of egg production in the 2 groups was 3.24% (P0.05) and 7.53% (P0.01) higher than that in the negative control group. The ratio of egg to eggs in the negative control group was 16.11% lower than that in the control group (P0.05). Test 1 groups, test 2 groups, 3 groups of material and egg ratio were lower than the negative control group, but the difference was not significant (P0.05). So in the production performance of the egg chicken, the optimal test group was 2 groups of egg quality: Test 1, 2 groups, 3 groups compared with the normal control group and negative control group, eggshell thickness, strength, egg weight, protein height, Hough unit And the color difference of egg yolk was not significant (P0.05).3. nutrient metabolism rate: adding compound enzyme preparation in low energy diet promoted dry matter metabolism rate, and the dry matter metabolism rate of 3 groups was significantly higher than that of negative control group, increased by 8.76% (P0.05), and there was no significant difference between the control group and the control group (P0.05). The crude protein metabolism of the 2 groups was increased by 5.77% (P0.05). The crude protein metabolism rate in the 3 groups was 10.60% higher than that in the normal control group (P0.01). The crude protein metabolism rate of the 2 groups and the 3 groups increased by 6.92% (P0.05), 11.80% (P0.01), and the enzyme preparation in the low energy diet was added. Promoting the apparent metabolic energy of feed, among the 1 groups, the apparent metabolic energy of the 3 groups was increased by 8.73% and 9.04%, respectively (P0.05), but the 1 groups and the 3 groups were significantly lower than the positive control group (P0.01). Therefore, the experimental group with better effect in the nutrient metabolism rate was the 3 groups of.4. blood indexes: the test 1, The serum albumin in the 3 groups was significantly higher than that in the positive control group (P0.01), and significantly higher than that in the negative control group (P0.01). The test 2 groups, 3 groups of serum globulin were significantly higher than the negative control group (P0.05), and significantly higher than that in the control group; the total protein content in the serum of 1 groups was significantly higher than that in the negative control group. The total serum protein content in the 3 groups was significantly higher than that of the negative control group (P0.01), and the total serum total cholesterol content in the 2 groups and the test groups was significantly higher than that of the positive control group and the negative control group (P0.05), and the serum triglyceride in the 1 groups was significantly lower than the negative control group in the test group (P0.05). (P0.05) and significantly lower than the control group (P0.01), test 2 groups, 3 groups of serum triglycerides compared with the negative control group, the difference was not significant (P0.05), but very significantly lower than the control group (P0.01). The enzyme preparation has little effect on the content of calcium in the serum, there is no significant difference between the groups (P0.05), the 1 groups of phosphorus in the test group are significantly higher than the positive pair. The content of phosphorus in the 3 groups was significantly higher than that of the control group and the negative control group (P0.01). To sum up, the addition of a certain amount of enzyme preparation could make up the negative effects of reducing energy on laying hens, and could improve the performance of the laying hens, increase the rate of nutrient metabolism, and promote the blood indexes. The overall comparison of the 200g/t laying hens was the overall comparison. The effect of production is better.
【學(xué)位授予單位】：沈陽農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S831.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 古金華;復(fù)合酶制劑飼喂斷奶仔豬效果試驗[J];養(yǎng)豬;2000年01期

2 劉英,劉洋;畜禽飼喂遼星牌復(fù)合酶的效果觀察[J];現(xiàn)代畜牧獸醫(yī);2005年09期

3 蔣宗勇,陳小薇,林映才,郭吉余;溢多利復(fù)合酶飼養(yǎng)黃雞效果的研究[J];中國飼料;1994年05期

4 高玉紅,孫新勝,郭衛(wèi)華;復(fù)合酶制劑對斷奶仔豬糞便菌群的影響[J];中國飼料;2001年03期

5 厲麗華,潘云河,王柏強(qiáng),余穎,項延潤;飼用復(fù)合酶喂豬的效果試驗[J];浙江畜牧獸醫(yī);2002年03期

6 ;天康復(fù)合酶面市[J];山東家禽;2002年08期

7 潘為忠;一種新型復(fù)合酶制劑——天康復(fù)合酶面市[J];當(dāng)代畜牧;2002年07期

8 潘為忠;一種新型復(fù)合酶制劑——天康復(fù)合酶面市[J];中國禽業(yè)導(dǎo)刊;2002年14期

9 張濤,李小麗;添加復(fù)合酶制劑對豬生長發(fā)育的影響[J];陜西農(nóng)業(yè)科學(xué);2003年05期

10 李紅玉;楊桂英;;復(fù)合酶制劑的生理功能及在動物生產(chǎn)中的應(yīng)用[J];飼料研究;2006年06期

相關(guān)會議論文 前10條

1 管武太;劉朝亮;;復(fù)合酶制劑對仔豬血液生理生化指標(biāo)的影響[A];酶制劑在飼料工業(yè)中的應(yīng)用[C];2005年

2 李兆勇;吳培均;;復(fù)合酶制劑的合理選擇與精細(xì)化應(yīng)用[A];2010山東飼料科學(xué)技術(shù)交流大會論文集[C];2010年

3 王敏;翁曉輝;李陽源;史寶軍;;飼用復(fù)合酶制劑對肉鴨生長性能的影響[A];中國畜牧獸醫(yī)學(xué)會動物營養(yǎng)學(xué)分會第十一次全國動物營養(yǎng)學(xué)術(shù)研討會論文集[C];2012年

4 呂東海;林海燕;劉亞力;王淑彩;王敏;;微丸型飼用復(fù)合酶在生長育肥豬飼料中的應(yīng)用效果研究[A];中國畜牧獸醫(yī)學(xué)會動物營養(yǎng)學(xué)分會——第九屆學(xué)術(shù)研討會論文集[C];2004年

5 趙德英;茌亞青;任德勝;張玉紅;;酶制劑飼喂產(chǎn)蛋期蛋雞的效果研究[A];2009山東飼料科學(xué)技術(shù)交流大會論文集[C];2009年

6 張青青;楊在賓;楊維仁;姜淑貞;張桂國;蕭貴松;;玉米-豆粕型日糧中添加耐高溫復(fù)合酶制劑對肉雞養(yǎng)分利用率的影響[A];2009山東飼料科學(xué)技術(shù)交流大會論文集[C];2009年

7 周華杰;;復(fù)合酶制劑在畜禽生產(chǎn)中的應(yīng)用進(jìn)展[A];2009山東飼料科學(xué)技術(shù)交流大會論文集[C];2009年

8 張海棠;王艷榮;胡慶鋒;;復(fù)合酶制劑對肉雞生產(chǎn)性能的影響[A];河南省畜牧獸醫(yī)學(xué)會第七屆理事會第二次會議暨2008年學(xué)術(shù)研討會論文集[C];2008年

9 段磊;趙國先;任志友;蘇坤;杜宗棠;計成;馬秋剛;張建云;鄭長山;;日糧中小麥和復(fù)合酶制劑對生長蛋雞養(yǎng)分表觀消化率的影響[A];第五屆（2011）中國蛋雞行業(yè)發(fā)展大會會刊[C];2011年

10 王平;;纖維素復(fù)合酶的作用及其在反芻動物生產(chǎn)上的應(yīng)用[A];2009山東飼料科學(xué)技術(shù)交流大會論文集[C];2009年

相關(guān)重要報紙文章 前6條

1 揚(yáng)州大學(xué)動物科學(xué)與技術(shù)學(xué)院 俞路 王雅倩 章世元;肉鴨添喂復(fù)合酶制劑生長快[N];江蘇農(nóng)業(yè)科技報;2008年

2 河北大學(xué)生命科學(xué)學(xué)院 馬利;飼用復(fù)合酶在畜禽上的應(yīng)用[N];河北科技報;2004年

3 康海;復(fù)合酶制劑喂雛雞增重快[N];江蘇農(nóng)業(yè)科技報;2004年

4 郭鳳蓮　陳存社;微生物制備復(fù)合酶制劑研究進(jìn)展[N];中國食品質(zhì)量報;2009年

5 高巖;FE復(fù)合酶：劃時代的高科技成果[N];光明日報;2000年

6 南京農(nóng)業(yè)大學(xué) 周巖民;飼料企業(yè)突破困境的路徑[N];中國畜牧報;2004年

相關(guān)博士學(xué)位論文 前3條

1 范成莉;β-葡聚糖、木聚糖復(fù)合酶對斷奶仔豬生長軸激素的影響及其機(jī)理研究[D];浙江大學(xué);2008年

2 湯海鷗;NSP復(fù)合酶優(yōu)化及在肉雞日糧中高劑量添加效果研究[D];中國農(nóng)業(yè)科學(xué)院;2013年

3 王紅梅;復(fù)合酶處理玉米秸稈對肉羊生產(chǎn)性能及破解纖維結(jié)構(gòu)機(jī)制的研究[D];中國農(nóng)業(yè)科學(xué)院;2017年

相關(guān)碩士學(xué)位論文 前10條

1 葉超;不同蛋白水平與復(fù)合酶對0~28日齡信鴿生產(chǎn)性能與消化功能的影響[D];福建農(nóng)林大學(xué);2015年

2 劉慧龍;復(fù)合酶對幾種飼料原料養(yǎng)分消化率的影響及其在豬飼料中的應(yīng)用[D];沈陽農(nóng)業(yè)大學(xué);2016年

3 李奎;酶制劑在肉牛日糧中的應(yīng)用效果研究[D];石河子大學(xué);2016年

4 唐千甯;高粱飼糧中添加復(fù)合酶和益生菌對肉雞的生長及養(yǎng)分消化率的影響[D];湖南農(nóng)業(yè)大學(xué);2016年

5 于翔宇;復(fù)合酶制劑的體外篩選及其在蛋雞低能日糧中的應(yīng)用[D];沈陽農(nóng)業(yè)大學(xué);2017年

6 張寶成;飼用纖維素復(fù)合酶在綿羊飼養(yǎng)中的作用效果及作用機(jī)理研究[D];新疆農(nóng)業(yè)大學(xué);2004年

7 武靜龍;復(fù)合酶制劑對獺兔生產(chǎn)性能和血清生理生化指標(biāo)的影響[D];湖南農(nóng)業(yè)大學(xué);2006年

8 周華杰;復(fù)合酶制劑對斷奶仔豬養(yǎng)分利用率和生長發(fā)育影響的研究[D];山東農(nóng)業(yè)大學(xué);2010年

9 Lamine Kouta Keita;復(fù)合酶制劑對肉雞生長性能、抗氧化、免疫及肉品質(zhì)的影響[D];南京農(nóng)業(yè)大學(xué);2013年

10 隋毅;不同復(fù)合酶制劑對肉仔雞生長性能、糞便相對黏度和菌群數(shù)量的影響[D];中國農(nóng)業(yè)科學(xué)院;2012年

，

本文編號：1909871