本文選題：荔枝蜜 + 棗花蜜�。� 參考：《西北大學》2017年碩士論文

【摘要】：荔枝蜜和棗花蜜是我國市場上較為常見的兩大單花種蜂蜜。棗花蜜多產(chǎn)自我國北方地區(qū),而荔枝蜜多產(chǎn)自南方地區(qū)。雖蜂蜜采自不同的植物源,色澤口感各有特色,但都具有豐富的營養(yǎng)價值。然而,目前對荔枝蜜和棗花蜜的研究卻有所不足,對花源標識物的研究以及在細胞水平對有害細胞的作用均鮮有報道。鑒別蜂蜜花源標識物是判別蜂蜜真假、區(qū)分摻假蜂蜜的一種重要手段。而細胞活性研究則對了解蜂蜜的藥用價值以及功能開發(fā)具有重要意義。因此,本課題圍繞荔枝蜜的花源標識物和棗花蜜對HepG2人肝癌細胞的促凋亡作用進行初步探究。1、構建了采自中國不同地區(qū)的13批荔枝蜜和13批棗花蜜的HPLC-DAD指紋圖譜,獲取相關系數(shù)矩陣數(shù)據(jù),并對樣品進行聚類分析。指紋圖譜結果顯示,荔枝蜜共有28個共有峰,棗花蜜共有32個共有峰。于荔枝蜜中發(fā)現(xiàn)2種化合物,棗花蜜中發(fā)現(xiàn)4種化合物可作為其花源標識物。聚類分析結果表明基于相關系數(shù)矩陣進行最遠距離聚類可在一定程度上分析樣品的地理源,為地理源差異研究提供支持。2、對荔枝蜜中發(fā)現(xiàn)的2種花源標識物(B1和B2)進行分離純化和結構鑒定。采用XAD-2吸附樹脂和半制備型液相色譜分離,分別得到兩種花源標識物22.5 mg和4.7 mg,經(jīng)Q/TOF-MS驗證,其分子量均為264,是脫落酸及其同分異構體。3、對荔枝蜜和棗花蜜的體外抗氧化活性進行研究,分別測定了總酚含量、DPPH·清除能力和氧自由基清除能力(ORAC)。結果顯示,棗花蜜的總酚含量高于荔枝蜜,均值為525.1±72.35 mg GAE/kg。棗花蜜DPPH·清除率(IC50)均值和ORAC均值為32.55±4.16 mg/mL和3.39±0.85 μmol TE/g,明顯高于荔枝蜜,且與總酚含量表現(xiàn)出正相關。因此,選擇棗花蜜為研究對象進行下一步研究。4、研究了棗花蜜對HepG2人肝癌細胞的促凋亡作用,并探究了其誘導凋亡的機制。結果顯示,經(jīng)棗花蜜處理的HepG2細胞活力會隨著棗花蜜濃度的增加而下降。棗花蜜可以引發(fā)HepG2細胞凋亡,使其細胞核發(fā)生破裂,凋亡特征明顯,且凋亡細胞比例與棗花蜜的濃度也表現(xiàn)出濃度正相關性。此外,棗花蜜會引起線粒體膜電位降低、增加Bax和Bad蛋白的表達量,降低Bcl-2和Bcl-xL蛋白的表達量。同時,抑癌基因p53的表達量也伴隨處理濃度增大而增加,caspase-9和下游蛋白caspase-3表達上調(diào),最終導致凋亡。可見,棗花蜜可能通過p53介導線粒體依賴途徑誘導HepG2細胞凋亡。
[Abstract]:Litchi honey and jujube nectar are two common single-flower honey in Chinese market. Jujube nectar comes from the north of China, while litchi honey from the south. Honey from different plant sources, color and taste have their own characteristics, but have rich nutritional value. However, the researches on litchi honey and jujube nectar are not enough, and there are few reports on the floral markers and the effects on harmful cells at the cell level. The identification of honey flower source marks is an important means to distinguish the true and false honey and to distinguish the adulteration of honey. The study of cell activity is of great significance to understand the medicinal value and function development of honey. Therefore, the effects of flower marker of litchi honey and jujube nectar on apoptosis of HepG2 human hepatoma cells were studied in this paper. The HPLC-DAD fingerprints of 13 batches of litchi honey and 13 batches of jujube nectar collected from different regions of China were constructed. The correlation coefficient matrix data were obtained and cluster analysis was carried out on the samples. The fingerprint showed that there were 28 peaks in litchi honey and 32 peaks in jujube nectar. Two compounds were found in litchi honey and four compounds in jujube nectar. The results of cluster analysis show that the remote clustering based on the correlation coefficient matrix can analyze the geographical source of the sample to a certain extent. To provide support for the study of geographical source difference. 2. To isolate and purify and identify the structure of two floral markers (B _ 1 and B _ 2) found in litchi honey. Two flower markers, 22.5 mg and 4.7 mg, were obtained by XAD-2 adsorption resin and semi-preparation liquid chromatography, respectively, and were verified by Q/TOF-MS. Its molecular weight is 264, which is abscisic acid and its isomer. 3. The antioxidant activities of litchi honey and jujube nectar in vitro were studied. The scavenging capacity of DPPH and oxygen free radical were determined respectively. The results showed that the total phenol content of jujube nectar was higher than that of litchi honey, and the mean value was 525.1 鹵72.35mg GAE / KG. The mean DPPH clearance rate (IC50) and ORAC of jujube nectar were 32.55 鹵4.16 mg/mL and 3.39 鹵0.85 渭 mol TEP 路g, which were significantly higher than that of litchi honey, and had a positive correlation with total phenol content. Therefore, the effect of jujube nectar on apoptosis of HepG2 human hepatoma cells was studied, and the mechanism of inducing apoptosis was explored. The results showed that the cell viability of HepG2 treated with jujube nectar decreased with the increase of jujube nectar concentration. Jujube nectar could induce the apoptosis of HepG2 cells, and the apoptosis characteristics were obvious. The proportion of apoptotic cells was positively correlated with the concentration of jujube nectar. In addition, jujube nectar could decrease mitochondrial membrane potential, increase the expression of Bax and Bad, and decrease the expression of Bcl-2 and Bcl-xL. At the same time, the expression of tumor suppressor gene p53 was increased with the increase of treatment concentration, and the expression of caspase-9 and downstream protein caspase-3 was up-regulated, which resulted in apoptosis. Therefore, jujube nectar may induce apoptosis of HepG2 cells through mitochondrial dependent pathway mediated by p53.
【學位授予單位】：西北大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S896.1;TS201.2

【相似文獻】

相關期刊論文 前10條

1 曾哲靈;高蔭榆;薛艷輝;;棗花蜜的熱穩(wěn)定性研究[J];食品科學;2006年11期

2 劉榮慶;新鄭棗花蜜[J];中國食品;1986年12期

3 林汝梅;;棗花蜜真?zhèn)蔚蔫b別方法[J];河北農(nóng)業(yè)科技;1986年12期

4 車振明,張?zhí)K勤,劉寶琦;棗花蜜口服液的研究[J];山西食品工業(yè);1994年04期

5 穆雪峰;孫麗萍;徐響;龐杰;何偉;黃蘭;沈新鋒;;棗花蜜中抗氧化成分提取工藝優(yōu)化[J];食品科學;2011年16期

6 鄧建軍;楊海霞;程妮;王畢妮;高慧;曹煒;;棗花蜜蛋白質(zhì)的電泳行為及其熒光光譜分析[J];食品與發(fā)酵工業(yè);2012年10期

7 周長文;趙文芳;謝尊逸;;棗花蜜及紅棗中溴氰菊酯殘留量的測定[J];農(nóng)藥;1986年03期

8 魏煒;加工食用蜂蜜各種指標的規(guī)定及控制[J];鄭州輕工業(yè)學院學報;1998年S2期

9 徐坤;任紅蕾;;不同種類蜂蜜的抗氧化作用研究[J];食品工業(yè);2008年05期

10 穆雪峰;孫麗萍;徐響;龐杰;;棗花蜜中清除DPPH自由基的活性成分[J];食品科學;2010年21期

相關會議論文 前1條

1 張?zhí)煜?郭禮和;蔡仙德;蘇寧;王立新;;反義RNA技術阻斷HEpG2細胞FasL的表達[A];中國免疫學會第四屆學術大會會議議程及論文摘要集[C];2002年

相關重要報紙文章 前5條

1 楊梵;若羌原生棗花蜜受青睞[N];農(nóng)民日報;2006年

2 范學風;蜂蜜的貯藏[N];河南科技報;2002年

3 孫林;蜂蜜,早晨吃更提精神[N];健康時報;2009年

4 白軼南 楊平凡;生吃蜂蜜營養(yǎng)更好[N];科技日報;2006年

5 菡馨;控制好溫度可延續(xù)蜂蜜結晶的進程[N];衛(wèi)生與生活報;2006年

相關碩士學位論文 前10條

1 周娟;棗花蜜理化指標及抗氧化活性研究[D];西北大學;2013年

2 穆雪峰;h花蜜中抗氧化成分的提取、測定及其活性研究[D];福建農(nóng)林大學;2012年

3 杜曉靜;三種單花種蜂蜜酚類標記物的確定及其應用[D];西北大學;2013年

4 梁小婷;馬錢子堿聯(lián)合5-氟尿嘧啶對HepG2細胞的抑制作用及其機制的研究[D];山西醫(yī)科大學;2017年

5 許義;基于hepG2細胞模型的丙烯酰胺危害性風險評估[D];上海師范大學;2014年

6 王盼;甲醛對HepG2細胞脂質(zhì)代謝相關通路影響的研究[D];山西醫(yī)科大學;2017年

7 王燦;芒果苷小檗堿鹽在HepG2細胞中調(diào)節(jié)糖脂代謝的作用研究[D];北京協(xié)和醫(yī)學院;2017年

8 尹姿升;懷化龍泉湖小區(qū)標識系統(tǒng)規(guī)劃設計[D];中南林業(yè)科技大學;2015年

9 秦軍立;基于標識物的虛實注冊與交互技術研究[D];北方工業(yè)大學;2016年

10 韓雪;基于三維標識物的醫(yī)用增強現(xiàn)實跟蹤注冊方法研究[D];吉林大學;2017年

，

本文編號：1790943