本文選題：卵殼蛋白 + CHO細(xì)胞　； 參考：《生物學(xué)雜志》2017年06期

【摘要】：已有研究表明卵殼蛋白ZPC5(Dissostichus mawsoni zona pellucida c5,Dm ZPC5)在南極魚卵巢中大量表達(dá),但是其具體功能尚不清楚。為了明確ZPC5是否參與魚類抗寒過程,研究在中國倉鼠卵巢細(xì)胞(Chinese hamster ovary,CHO)中過表達(dá)南極魚ZPC5,隨之給予CHO細(xì)胞一定程度的低溫處理,通過統(tǒng)計(jì)CHO細(xì)胞的存活率,來探討zpc5在魚類抗寒過程中的作用。存活率檢測(cè)結(jié)果發(fā)現(xiàn),過表達(dá)ZPC5的CHO細(xì)胞在0℃處理16 h后,其存活率較對(duì)照組顯著上升(P0.001),證明zpc5在低溫下可以促進(jìn)細(xì)胞的存活。Western Blot檢測(cè)結(jié)果顯示,zpc5能在CHO細(xì)胞中高效表達(dá),0℃處理后,CHO細(xì)胞內(nèi)的ZPC5表達(dá)量顯著上調(diào),證明了低溫可以誘導(dǎo)ZPC5的表達(dá)。研究結(jié)果表明,ZPC5有可能參與南極魚魚卵抗寒過程。
[Abstract]:It has been shown that ZPC5(Dissostichus mawsoni zona pellucida c5Dm ZPC5 is highly expressed in Antarctic fish ovary, but its specific function is still unclear.In order to determine whether ZPC5 is involved in the chilling resistance of fish, the expression of ZPC5 in Chinese hamster ovaries was studied in Chinese hamster ovary cells, and then CHO cells were treated with a certain degree of hypothermia, and the survival rate of CHO cells was calculated.To explore the role of zpc5 in the cold resistance of fish.The results of survival test showed that CHO cells expressing ZPC5 were treated at 0 鈩，

本文編號(hào)：1732583