本文選題：鹽穗木　切入點：正丁醇萃取物　出處：《塔里木大學》2017年碩士論文

【摘要】：鹽穗木(Halostachys caspica)是荒漠主要的植物之一,是隸屬于藜科植物,對鹽土地有較為良好的耐受性。早年已有研究發(fā)現(xiàn)鹽穗木的正丁醇萃取物對金黃色葡萄球菌有較好的抑制生長的效果,因而本實驗通過MIC和MBC的測定、St.aureus生長曲線的測定、纖維結(jié)合蛋白試驗、電導率測定法、培養(yǎng)液中蛋白質(zhì)濃度測定、培養(yǎng)液中還原糖濃度測定、β-半乳糖苷酶活力測定法、堿性磷酸酶測定、胞內(nèi)Ca2+濃度測定等,測定并比較不同溶劑鹽穗木萃取物的抑菌活性進行;為明確金黃色葡萄球菌細胞膜的滲透性,我們測定了金黃色葡萄球細胞內(nèi)抗氧化酶酶活性測定、金黃色葡萄球菌酶液粗測定、金黃色葡萄球菌細胞內(nèi)源H2O2含量的測定、金黃色葡萄球菌細胞脂質(zhì)過氧化測定;最后,明確鹽穗木正丁醇萃取物對金黃色葡萄球菌細胞形態(tài)改變的影響。試驗研究得到如下結(jié)果:1、鹽穗木含有多種提取的化學成分,其中主要包括糖、多糖、多肽、氨基酸、有機酸、甾體、三萜類物質(zhì)、皂苷、酚類、蹂質(zhì)、生物堿、黃酮、揮發(fā)油等;鹽穗木正丁醇萃取物對金黃色葡萄球菌生長曲線的影響,主要體現(xiàn)在金黃色葡萄球菌生長的對數(shù)時期;同時,對金黃色葡萄球菌,鹽穗木正丁醇萃取物的抑菌效果較為良好,其最低殺菌濃度(MBC)為0.1250 mg/mL,最小抑菌濃度(MIC)為0.0625 mg/mL,且比對照組提前7h進入穩(wěn)定期。2、含有正丁醇萃取物的藥液經(jīng)100℃處理1h,抑菌率80%,說明該活性物質(zhì)的熱穩(wěn)定性較好,可配合高溫設備的清洗殺菌。正丁醇萃取物在pH值7-8環(huán)境中所具有的抑菌活性最高,抑菌率90%,有望用于容易感染金黃色葡萄球菌的各種肉類、水產(chǎn)、蛋類等pH值中性食品的防腐保鮮。3、正丁醇萃取物所致的細胞膜通透性的改變,會引起溶液外物質(zhì)濃度、種類、胞外電導率的變化,這將引起細胞內(nèi)鉀鈉離子等小分子物質(zhì)的外漏,當細胞膜破壞嚴重時,將造成永久性傷害,這時會出現(xiàn)胞內(nèi)蛋白質(zhì)等大分子物質(zhì)漏到胞外基質(zhì)中,隨著效果時間的延長,細胞膜將被正丁醇萃取完全破壞,培養(yǎng)液電導率將胞內(nèi)物質(zhì)釋放到胞外基質(zhì)快速上升。
[Abstract]:Halostachys caspicia is one of the main plants in desert and belongs to the family Chenopodiaceae. Halostachys caspicia has good tolerance to salt soil.In early years, it was found that n-butanol extract from salt-spikelet had a good inhibitory effect on the growth of Staphylococcus aureus. Therefore, the growth curve of St.aureus was determined by MIC and MBC, fibronectin test and electrical conductivity test were used to determine the growth curve of Staphylococcus aureus.Protein concentration in culture medium, reducing sugar concentration in culture medium, 尾 -galactosidase activity, alkaline phosphatase and intracellular Ca2 concentration were measured and compared.In order to determine the permeability of staphylococcus aureus cell membrane, we measured the activity of antioxidant enzymes in the cells of grape ball, the crude enzyme solution of Staphylococcus aureus, and the content of endogenous H2O2 in the cells of Staphylococcus aureus.Determination of lipid peroxidation of Staphylococcus aureus cells. Finally, the effect of n-butanol extract from Staphylococcus aureus on the morphological changes of Staphylococcus aureus cells was determined.The results of the experiment are as follows: 1: 1. There are many kinds of chemical constituents, including sugar, polysaccharides, polypeptides, amino acids, organic acids, steroids, triterpenes, saponins, phenols, ragged substances, alkaloids, flavonoids, etc.The effect of n-butanol extract on the growth curve of Staphylococcus aureus is mainly reflected in the logarithmic period of the growth of Staphylococcus aureus.The antimicrobial effect of n-butanol extract from salt-panicle was good.It can be combined with the cleaning and sterilization of high temperature equipment.The n-butanol extract has the highest bacteriostatic activity in pH 7-8 environment, and the bacteriostatic rate is 90%, which is expected to be used in various kinds of meat and aquatic products susceptible to infection with Staphylococcus aureus.The change of cell membrane permeability caused by n-butanol extract may cause the change of extracellular concentration, species and extracellular conductivity of neutral food with pH value such as eggs.This will cause leakage of small molecules such as potassium, sodium ions and other small molecules in the cell. When the cell membrane is seriously damaged, it will cause permanent damage. At this time, macromolecular substances such as intracellular proteins will leak into the extracellular matrix, and with the prolongation of the effect time,The membrane was completely destroyed by n-butanol extraction, and the electrical conductivity of the medium released the intracellular substance to the extracellular matrix.
【學位授予單位】：塔里木大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S853.7

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