本文選題：鹽穗木　切入點(diǎn)：正丁醇萃取物　出處：《塔里木大學(xué)》2017年碩士論文

【摘要】：鹽穗木(Halostachys caspica)是荒漠主要的植物之一,是隸屬于藜科植物,對(duì)鹽土地有較為良好的耐受性。早年已有研究發(fā)現(xiàn)鹽穗木的正丁醇萃取物對(duì)金黃色葡萄球菌有較好的抑制生長(zhǎng)的效果,因而本實(shí)驗(yàn)通過(guò)MIC和MBC的測(cè)定、St.aureus生長(zhǎng)曲線的測(cè)定、纖維結(jié)合蛋白試驗(yàn)、電導(dǎo)率測(cè)定法、培養(yǎng)液中蛋白質(zhì)濃度測(cè)定、培養(yǎng)液中還原糖濃度測(cè)定、β-半乳糖苷酶活力測(cè)定法、堿性磷酸酶測(cè)定、胞內(nèi)Ca2+濃度測(cè)定等,測(cè)定并比較不同溶劑鹽穗木萃取物的抑菌活性進(jìn)行;為明確金黃色葡萄球菌細(xì)胞膜的滲透性,我們測(cè)定了金黃色葡萄球細(xì)胞內(nèi)抗氧化酶酶活性測(cè)定、金黃色葡萄球菌酶液粗測(cè)定、金黃色葡萄球菌細(xì)胞內(nèi)源H2O2含量的測(cè)定、金黃色葡萄球菌細(xì)胞脂質(zhì)過(guò)氧化測(cè)定;最后,明確鹽穗木正丁醇萃取物對(duì)金黃色葡萄球菌細(xì)胞形態(tài)改變的影響。試驗(yàn)研究得到如下結(jié)果:1、鹽穗木含有多種提取的化學(xué)成分,其中主要包括糖、多糖、多肽、氨基酸、有機(jī)酸、甾體、三萜類物質(zhì)、皂苷、酚類、蹂質(zhì)、生物堿、黃酮、揮發(fā)油等;鹽穗木正丁醇萃取物對(duì)金黃色葡萄球菌生長(zhǎng)曲線的影響,主要體現(xiàn)在金黃色葡萄球菌生長(zhǎng)的對(duì)數(shù)時(shí)期;同時(shí),對(duì)金黃色葡萄球菌,鹽穗木正丁醇萃取物的抑菌效果較為良好,其最低殺菌濃度(MBC)為0.1250 mg/mL,最小抑菌濃度(MIC)為0.0625 mg/mL,且比對(duì)照組提前7h進(jìn)入穩(wěn)定期。2、含有正丁醇萃取物的藥液經(jīng)100℃處理1h,抑菌率80%,說(shuō)明該活性物質(zhì)的熱穩(wěn)定性較好,可配合高溫設(shè)備的清洗殺菌。正丁醇萃取物在pH值7-8環(huán)境中所具有的抑菌活性最高,抑菌率90%,有望用于容易感染金黃色葡萄球菌的各種肉類、水產(chǎn)、蛋類等pH值中性食品的防腐保鮮。3、正丁醇萃取物所致的細(xì)胞膜通透性的改變,會(huì)引起溶液外物質(zhì)濃度、種類、胞外電導(dǎo)率的變化,這將引起細(xì)胞內(nèi)鉀鈉離子等小分子物質(zhì)的外漏,當(dāng)細(xì)胞膜破壞嚴(yán)重時(shí),將造成永久性傷害,這時(shí)會(huì)出現(xiàn)胞內(nèi)蛋白質(zhì)等大分子物質(zhì)漏到胞外基質(zhì)中,隨著效果時(shí)間的延長(zhǎng),細(xì)胞膜將被正丁醇萃取完全破壞,培養(yǎng)液電導(dǎo)率將胞內(nèi)物質(zhì)釋放到胞外基質(zhì)快速上升。
[Abstract]:Halostachys caspicia is one of the main plants in desert and belongs to the family Chenopodiaceae. Halostachys caspicia has good tolerance to salt soil.In early years, it was found that n-butanol extract from salt-spikelet had a good inhibitory effect on the growth of Staphylococcus aureus. Therefore, the growth curve of St.aureus was determined by MIC and MBC, fibronectin test and electrical conductivity test were used to determine the growth curve of Staphylococcus aureus.Protein concentration in culture medium, reducing sugar concentration in culture medium, 尾 -galactosidase activity, alkaline phosphatase and intracellular Ca2 concentration were measured and compared.In order to determine the permeability of staphylococcus aureus cell membrane, we measured the activity of antioxidant enzymes in the cells of grape ball, the crude enzyme solution of Staphylococcus aureus, and the content of endogenous H2O2 in the cells of Staphylococcus aureus.Determination of lipid peroxidation of Staphylococcus aureus cells. Finally, the effect of n-butanol extract from Staphylococcus aureus on the morphological changes of Staphylococcus aureus cells was determined.The results of the experiment are as follows: 1: 1. There are many kinds of chemical constituents, including sugar, polysaccharides, polypeptides, amino acids, organic acids, steroids, triterpenes, saponins, phenols, ragged substances, alkaloids, flavonoids, etc.The effect of n-butanol extract on the growth curve of Staphylococcus aureus is mainly reflected in the logarithmic period of the growth of Staphylococcus aureus.The antimicrobial effect of n-butanol extract from salt-panicle was good.It can be combined with the cleaning and sterilization of high temperature equipment.The n-butanol extract has the highest bacteriostatic activity in pH 7-8 environment, and the bacteriostatic rate is 90%, which is expected to be used in various kinds of meat and aquatic products susceptible to infection with Staphylococcus aureus.The change of cell membrane permeability caused by n-butanol extract may cause the change of extracellular concentration, species and extracellular conductivity of neutral food with pH value such as eggs.This will cause leakage of small molecules such as potassium, sodium ions and other small molecules in the cell. When the cell membrane is seriously damaged, it will cause permanent damage. At this time, macromolecular substances such as intracellular proteins will leak into the extracellular matrix, and with the prolongation of the effect time,The membrane was completely destroyed by n-butanol extraction, and the electrical conductivity of the medium released the intracellular substance to the extracellular matrix.
【學(xué)位授予單位】：塔里木大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S853.7

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