本文選題：甜瓜　切入點(diǎn)：蔓枯病　出處：《新疆大學(xué)》2017年碩士論文

【摘要】：甜瓜是一年生蔓性草本植物,在我國(guó)各地廣泛分布,是深受人們喜愛(ài)的重要水果之一。甜瓜蔓枯病是由Didymella bryoniae引起的真菌病害,其田間的爆發(fā)常導(dǎo)致甜瓜葉片、莖部、根部、果實(shí)受損,因此導(dǎo)致甜瓜減產(chǎn)、品質(zhì)降低。本研究采用光學(xué)顯微鏡鏡檢、病原菌致病力分析、真菌ITS區(qū)分子測(cè)序鑒定結(jié)合的方法,對(duì)實(shí)驗(yàn)中使用的蔓枯病病原菌進(jìn)行鑒定,確定了該真菌的歸屬。對(duì)實(shí)驗(yàn)室保存的14種甜瓜鑒別寄主與感病品種Hami413,通過(guò)苗期人工噴霧分生孢子法篩選出蔓枯病抗性甜瓜材料。以篩選出的抗蔓枯病甜瓜品種Iran H與感蔓枯病甜瓜品種Hami413為親本,構(gòu)建F2代分離群體,利用BSA法(混合分組分析法)和SSR分子標(biāo)記技術(shù),篩選出甜瓜抗病池與感病池之間擴(kuò)增出差異條帶的SSR分子標(biāo)記,為Iran H抗蔓枯病基因的定位奠定基礎(chǔ)。主要結(jié)果如下:(1)感蔓枯病甜瓜品種Hami413的接種結(jié)果表明:該菌株為致病性較強(qiáng)的菌株;且與已報(bào)道的D.bryoniae菌株的菌落及分生孢子形態(tài)相似;核糖體序列間隔區(qū)的測(cè)序結(jié)果經(jīng)Blast序列對(duì)比,進(jìn)一步證實(shí)該菌株屬于D.bryoniae。(2)苗期人工噴霧分生孢子法接種鑒定,篩選出高抗蔓枯病甜瓜材料Iran H,抗性材料在不同的季節(jié)抗性表現(xiàn)穩(wěn)定。對(duì)接種后對(duì)抗感材料葉片POD的活性指標(biāo)進(jìn)行測(cè)定,接種后抗病甜瓜葉片中的POD活性顯著高于感病甜瓜,可為甜瓜蔓枯病的接種材料鑒定作為參考。(3)以篩選出的抗蔓枯病甜瓜品種Iran H為供體親本,以感蔓枯病甜瓜品種Hami413為受體親本,構(gòu)建BC1及F2代分離群體,分離群體接種結(jié)果符合單個(gè)隱形基因的遺傳規(guī)律。(4)以128株F2代分離群體為材料,利用BSA法篩選出甜瓜抗病池與感病池之間擴(kuò)增出差異條帶的10個(gè)SSR分子標(biāo)記。
[Abstract]:Muskmelon is an annual tendril herb, which is widely distributed all over China and is one of the most popular important fruits. Melon vine blight is a fungal disease caused by Didymella bryoniae, and its outbreak in the field often results in the leaves, stems and roots of muskmelon. The fruit was damaged, which resulted in the decrease of yield and quality of muskmelon. In this study, microscopical examination, pathogenicity analysis of pathogenic bacteria and molecular sequencing of fungal ITS region were used to identify the pathogen of vine blight in the experiment. The identification of 14 varieties of muskmelon cultivars, Hami413, which were preserved in laboratory, was used to screen the resistant melon materials by artificial spray conidium method at seedling stage, and the resistant varieties of muskmelon were screened out by the method of artificial spray conidial spores at the seedling stage. Iran H and Hami413 were parents. Using BSA method (mixed grouping analysis) and SSR molecular marker technique, the SSR molecular markers with different bands were amplified between resistant and susceptible pools of muskmelon. The main results were as follows: (1) the inoculation results of Iran H susceptible muskmelon variety Hami413 showed that the strain was highly pathogenic and similar to the reported D.bryoniae strains in colony and conidial morphology. The sequencing results of ribosomal spacer region further confirmed that the strain belonged to D. bryoniae.2) inoculation with artificial spray conidium method at seedling stage. The resistant melon (Iran H) with high resistance to vine blight was selected, and the resistant material showed stable resistance in different seasons. The activity index of POD in resistant melon leaves after inoculation was significantly higher than that in susceptible melon leaves after inoculation, and the activity of POD in resistant melon leaves was significantly higher than that in susceptible melon leaves after inoculation. It can be used as reference for identification of inoculation material of melon vine blight disease. The isolated populations of BC1 and F2 generation were constructed by using Iran H as donor parent and Hami413 as recipient parent. The inoculation results of the isolated populations were in accordance with the genetic rule of a single invisible gene. (4) using 128 F _ 2 segregated populations as materials, 10 SSR molecular markers with differential bands were amplified from resistant and susceptible pools of muskmelon by BSA method.
【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S436.5

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