本文選題：紫薇　切入點(diǎn)：花色遺傳　出處：《山東農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：紫薇(Lagerstroemia Indica),千屈菜科紫薇屬,是重要的園林觀賞植物。本研究通過對四倍體紫薇‘四海升平’與其它品種雜交后代的花色性狀表現(xiàn)、遺傳多樣性分析等方面的研究,對多個(gè)雜交組合的遺傳多樣性進(jìn)行比較,在分子水平上研究多倍體紫薇的花色遺傳;通過對不同抗白粉病品種紫薇的生理指標(biāo)進(jìn)行測量、對比和分析,以明確不同侵染時(shí)間下紫薇葉片中超氧化物歧化酶活性,可溶性蛋白質(zhì)、丙二醛含量的變化規(guī)律,為白粉病抗性機(jī)制的研究提供依據(jù),并對抗病品種雜交后代進(jìn)行抗病評價(jià)。主要研究結(jié)果如下:(1)在‘紫抗1號’與‘四海升平’雜交組合中,父母本花色近似,子代中大部分均表現(xiàn)出親本的花色,同時(shí)也出現(xiàn)了異于親本的玫紅色與紫紅色;在‘紫抗2號’與‘四海升平’、‘泮河2號’與‘四海升平’雜交組合中,分別出現(xiàn)了與母本、父本花色相近的顏色,以及介于二者之間的花色。各雜交組合后代中相同花色均表現(xiàn)出由淺到深的分離。(2)在三個(gè)雜交組合中,每個(gè)組合的子代中均出現(xiàn)了親本共有條帶,也具有單個(gè)親本獨(dú)有的條帶�！峡�1號’ב四海升平’、‘紫抗2號’ב四海升平’雜交組合的子代具有母本特異條帶百分率高于具有父本特異條帶百分率,屬于偏母本類型;‘泮河2號’ב四海升平’雜交組合的子代父本特異條帶百分率高于母本特異條帶百分率,屬于偏父型。三個(gè)雜交組合遺傳多樣性值水平的范圍分別為H(0.209-0.2299)、I(0.345-0.3689),差別不大,說明3個(gè)組合均具有較高的多態(tài)性及較高水平的遺傳多樣性。三個(gè)雜交組合遺傳多樣性由大到小分別為:‘泮河2號’ב四海升平’‘紫抗1號’ב四海升平’‘紫抗2號’ב四海升平’。聚類結(jié)果表明:以相似系數(shù)0.77處取結(jié)合線,可將43份材料劃分為3類,其中紫色、紅色單株各自聚在一起,粉色單株聚類較分散。(3)抗病與免疫品種‘紫抗1號’、‘紫抗2號’的SOD活性高于感病品種‘四海升平’,且抗病與免疫品種的SOD修復(fù)快于感病品種;感病品種MDA含量不斷上升,抗病品種與免疫品種的含量與變化趨勢相近,變化較小,并始終低于感病品種;三類品種可溶性蛋白質(zhì)含量變化趨勢及含量變化基本一致,呈上升后下降的趨勢。(4)共評價(jià)子代72株,其中獲得高抗子代19株,中抗子代14株,中感子代17株,高感子代22株,并未發(fā)現(xiàn)免疫子代。通過本次雜交育種試驗(yàn),證實(shí)了通過紫薇雜交育種獲取抗病性的可行性。通過多個(gè)抗病雜交組合的對比,抗病雜交育種中應(yīng)優(yōu)先選取‘紫抗1號’作為母本。
[Abstract]:Lagerstroemia Indica, one of the most important ornamental plants in gardens, was studied in this paper. The genetic diversity of multiple cross combinations was compared to study the flower color inheritance of polyploid Lagerstroemia on the molecular level, and the physiological indexes of different powdery mildew resistant varieties were measured, compared and analyzed. The changes of superoxide dismutase (SOD) activity, soluble protein and malondialdehyde (MDA) content in the leaves of Lagerstroemia chinensis were determined under different infection time, which provided the basis for the study of resistance mechanism of powdery mildew. The main results are as follows: (1) in the cross combinations of 'Zikang 1' and 'Sihai Shengping', the parents' flowers and colors were similar, and most of the progenies showed the color of their parents. At the same time, different from their parents, rose red and purplish red appeared, and in the hybrid combinations of 'Zikang 2' and 'Sihai Shengping' Pan-he 2 and 'Sihai Shengping', the colors were similar to those of female parent and male parent, respectively. The same flower color in the progenies of each cross showed the separation from shallow to deep. In the three cross combinations, there was a common band of parents in the progeny of each combination. The specific band percentage of female parent was higher than that of male parent in the hybrid combination of 'Zikang 1' 脳'脳 'Zikang 2' 脳 'Sihaishengping'. The percentage of specific bands of paternal parents in the cross combinations of'脳 'Sihai Shengping' 2 'Panhe' type of female parent was higher than that of female parent, and the percentage of specific bands was higher than that of female parent's. The range of genetic diversity values of the three cross combinations was 0.209-0.2299. the genetic diversity level of the three crosses was 0.345-0.3689, and the difference was not significant. The genetic diversity of the three cross combinations is from big to small to the size of 1'脳 'Zikang' 1'脳 'Sihaiping 1' 脳 'Zikang' 2, respectively, from the big to the small. The genetic diversity of the three crosses is 1'脳 1'脳'脳 'Zikang 2' 脳 'Pan-he 2' 脳'脳 'Sihai Shengping 1' 脳'脳' The cluster results show that the binding line is taken at 0.77 of similarity coefficient. The 43 materials can be divided into three categories, purple and red trees gathered together, The SOD activity of disease resistant and immunized variety 'Zikang 1' and 'Zikang 1' Zikang 2 'was higher than that of susceptible variety' Sihai Shengping', and the SOD repair of resistant and immunized variety was faster than that of susceptible variety, and the MDA content of susceptible variety was increasing. The content of soluble protein in resistant and immunized varieties was similar to that in susceptible varieties, and the change was small, and the content of soluble protein in three varieties was basically the same. A total of 72 progenies were evaluated, of which 19 were of high resistance, 14 were moderately resistant, 17 were middle susceptible, and 22 were highly susceptible. No immune progeny was found. It was proved that it was feasible to obtain disease resistance by hybrid breeding of Lagerstroemia, and 'Zikang 1' should be selected as female parent in disease resistance hybrid breeding.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S685.99

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 聶碩;張林;王峰;孫忠奎;朱翠翠;謝憲;王長憲;;紫薇雜種F_1代的性狀表現(xiàn)與倍性研究[J];山東農(nóng)業(yè)科學(xué);2017年01期

2 王曉明;曾慧杰;李永欣;王湘瑩;喬中全;蔡能;;‘曉明一號’紫薇雜交育種研究[J];湖南林業(yè)科技;2016年06期

3 曾慧杰;王曉明;李永欣;蔡能;喬中全;許歡;;不育紫薇花粉活力及柱頭可授性研究[J];湖南林業(yè)科技;2016年05期

4 沈鴻明;侯伯鑫;張曉杰;薛桂芳;朱王微;王金鳳;陳卓梅;;浙江省紫薇品種資源調(diào)查Ⅰ—堇薇品種群[J];浙江林業(yè)科技;2016年04期

5 聶碩;張林;王峰;孫忠奎;朱翠翠;謝憲;王長憲;;紫薇種子輻射變異和抗性初步研究[J];農(nóng)學(xué)學(xué)報(bào);2016年05期

6 王淑安;王鵬;楊如同;汪慶;李林芳;李亞;尹增芳;;紫薇新品種‘金幌’[J];園藝學(xué)報(bào);2016年03期

7 王萱;劉曉靜;邢世巖;孔倩倩;張艷輝;孫立民;高燕;;中國部分古銀杏資源遺傳多樣性的AFLP分析及核心種質(zhì)的構(gòu)建[J];園藝學(xué)報(bào);2016年02期

8 范輝華;李乾振;湯行昊;姚湘明;張?zhí)煊?;福建省紫薇種質(zhì)資源調(diào)查與優(yōu)良單株選擇[J];林業(yè)資源管理;2015年05期

9 張西西;李俊;趙正楠;;紫茉莉品系觀賞性狀與遺傳多樣性的AFLP分析[J];北京農(nóng)學(xué)院學(xué)報(bào);2016年01期

10 楊樹華;郭寧;劉海星;葛紅;;新疆天山地區(qū)彎刺薔薇居群遺傳多樣性AFLP分析[J];園藝學(xué)報(bào);2015年09期

相關(guān)會議論文 前3條

1 張恩亮;王鵬;李亞;王淑安;李林芳;楊如同;;紫薇EST-SSR標(biāo)記的開發(fā)和利用[A];中國觀賞園藝研究進(jìn)展2016[C];2016年

2 張秦英;劉莉;;紫薇四倍體離體誘導(dǎo)[A];2008年園藝植物染色體倍性操作與遺傳改良學(xué)術(shù)研討會論文摘要集[C];2008年

3 張斌;李志輝;張冬林;王敏姿;;N~+注入對紫薇光合特性和葉綠素含量的影響[A];中國遺傳學(xué)會第十屆全國激光生物學(xué)學(xué)術(shù)會議論文摘要集[C];2009年

相關(guān)博士學(xué)位論文 前1條

1 王獻(xiàn);我國紫薇種質(zhì)資源及其親緣關(guān)系的研究[D];北京林業(yè)大學(xué);2004年

相關(guān)碩士學(xué)位論文 前10條

1 原蒙蒙;紫薇種間雜交及‘粉嬌容’種子輻射育種研究[D];河南農(nóng)業(yè)大學(xué);2015年

2 高麗琴;江西紫薇品種分類研究[D];江西農(nóng)業(yè)大學(xué);2014年

3 楊冰潔;幾種紫薇屬植物染色體制片技術(shù)優(yōu)化及原位雜交體系的建立[D];北京林業(yè)大學(xué);2013年

4 王曉嬌;紫薇同源四倍體的獲得及組培快繁技術(shù)研究[D];北京林業(yè)大學(xué);2013年

5 秦萌;紫薇花粉活力、貯藏方法及輻射劑量對種子萌發(fā)影響的研究[D];河南農(nóng)業(yè)大學(xué);2013年

6 陳磊;紫薇無性系建立及多倍體誘導(dǎo)技術(shù)研究[D];天津大學(xué);2011年

7 田娟;20個(gè)紫薇品種抗寒性比較研究[D];北京林業(yè)大學(xué);2009年

8 宋平;紫薇再生體系的建立及多倍體誘導(dǎo)研究[D];北京林業(yè)大學(xué);2009年

9 張照坤;紫薇生殖生物學(xué)及數(shù)量性狀遺傳研究[D];山東農(nóng)業(yè)大學(xué);2008年

10 田苗;我國紫薇新品種DUS測試指南及已知品種數(shù)據(jù)庫的研究[D];北京林業(yè)大學(xué);2008年

，

本文編號：1633590