本文選題：鐵皮石斛　切入點：UV-B輻射　出處：《廣東藥科大學》2017年碩士論文　論文類型：學位論文

【摘要】：本課題組前期研究發(fā)現(xiàn),UV-B輻射能影響鐵皮石斛苗的生長和活性成分的含量。茉莉酸甲酯是植物受到外界刺激時反應最迅速的信號分子,參與植物生長發(fā)育的各個過程以提高植物對逆境的適應性。本論文探討了UV-B輻射下茉莉酸甲酯對鐵皮石斛生長和主要活性成分的影響,實驗方案及結果如下:本實驗以鐵皮石斛組培苗為研究對象,在能造成鐵皮石斛苗傷害的UV-B輻射劑量下(15.6μW·cm-2)下,以MS為培養(yǎng)基,外源添加濃度為5mmol/L的茉莉酸甲酯,然后分別進行持續(xù)3、6、9、12小時的UV-B照射。考察鐵皮石斛苗的光合色素含量、抗氧化酶活性以及主要活性成分的變化,然后選擇該濃度茉莉酸甲酯能緩解UV-B輻射傷害及提高其活性成分含量的合適時間,進行8天周期性UV-B輻射實驗�？疾觳煌瑵舛鹊能岳蛩峒柞﹁F皮石斛周期性UV-B輻照的響應。各實驗處理中,噴施不同濃度的茉莉酸甲酯后,立即進行每天6小時的UV-B照射,茉莉酸甲酯濃度分別為:0 mmol/L(簡稱UV-B)、1 mmol/L(簡稱UV-B+MeJA1,下同)、2.5 mmol/L(UV-B+MeJA2.5)、5 mmol/L(UV-B+MeJA5)、7.5 mmol/L(UV-B+MeJA7.5)、10 mmol/L(UV-B+MeJA10)。在實驗的第2、4、6、8天,分別通過考察鐵皮石斛苗的萌蘗數(shù)、葉片破壞率、光合色素含量、抗氧化酶活性以及主要活性成分的變化,以研究茉莉酸甲酯對鐵皮石斛周期性UV-B輻射的響應持續(xù)時間和濃度。在此基礎上,探討UV-B輻射下,茉莉酸甲酯對兩個抗逆性存在明顯差異的鐵皮石斛品種的影響是否存在差異。主要結果如下:(1)短時間UV-B輻射下茉莉酸甲酯對鐵皮石斛的影響在UV-B持續(xù)照射下,鐵皮石斛苗的光合色素含量減少,而添加茉莉酸甲酯能提高光合色素的含量。UV-B+MeJA組的POD、CAT含量明顯高于CK和UV-B組,其含量分別較CK增加40%、55%、55%,且丙二醛含量較CK低,總多糖的含量較CK和UV-B組顯著增加(P0.05)。在實驗處理3、6、9、12小時后其含量較CK增加568%、307%、47%、38%。隨著時間的延長,而UV-B+MeJA組總黃酮含量在3小時含量較CK低,在6小時后含量逐漸增加。在實驗處理6小時后,UV-B+MeJA葉綠素a含量較CK顯著增加(P0.05),含量較CK增加38.2%。同時鐵皮石斛苗葉綠素b、類胡蘿卜素含量逐漸增加。(2)周期性UV-B照射下茉莉酸甲酯對鐵皮石斛的影響UV-B照射下,不同濃度的茉莉酸甲酯對鐵皮石斛苗葉片的影響程度不一樣。隨著茉莉酸甲酯濃度的提高,對UV-B輻射造成的葉片破壞緩解能力降低。在照射的第8天,不同濃度茉莉酸甲酯,對葉片的破壞率分別是100%、87.9%、51%、84.4%、83%、100%。其中UV-B+MeJA5組的葉片破壞率最小。此外,UV-B+MeJA1、UV-B+MeJA2.5、UV-B+MeJA5、UV-B+MeJA7.5和UV-B+MeJA10組萌蘗數(shù)前期增加,但在后期萌蘗受阻;而UV-B+MeJA2.5和UV-B+MeJA5組的萌蘗數(shù)在呈緩慢上升的趨勢。在實驗處理的前6天,鐵皮石斛苗光合色素含量增加較多;在實驗處理的6天后鐵皮石斛苗光合色素含量降低。同時,不同濃度的茉莉酸甲酯對鐵皮石斛苗的光合色素含量影響不同,UV-B+MeJA5和UV-B+MeJA7.5組光合色素含量始終高于UV-B、UV-B+MeJA1、UV-B+MeJA2.5和UV-B+MeJA10組。UV-B+MeJA5和UV-B+MeJA7.5組中的抗氧化酶活性較UV-B顯著增加(P0.05)。此外,UV-B+MeJA1、UV-B+MeJA2.5和UV-B+MeJA5的MDA含量均較UV-B低,UV-B+MeJA7.5和UV-B+MeJA10處理組的MDA含量較高�？梢姷蜐舛鹊能岳蛩峒柞ピ谝欢ǔ潭壬峡梢詼p輕UV-B輻射對膜脂過氧化產(chǎn)生的傷害。對鐵皮石斛主要活性成分的影響:在實驗處理的第4天,UV-B+MeJA5和UV-B+MeJA7.5組總多糖含量大于UV-B組(P0.05),其含量分別為UV-B組的108%和105%。此外,在實驗處理的第2、4、6天時,UV-B+MeJA2.5的總黃酮含量分別為UV-B組的86%、106%、41%。在實驗處理第2天時,UV-B+MeJA5處理中總黃酮影響不顯著(P0.05),在實驗處理第2、6天總黃酮含量分別為UV-B的100%和40%。(3)UV-B輻射下,兩個鐵皮石斛品種對外源茉莉酸甲酯的響應兩個品種的鐵皮石斛苗在UV-B照射下用茉莉酸甲酯處理,光合色素含量增加。鐵皮石斛YQ-2品種的光合色素含量、抗氧化酶活性較YH-1品種鐵皮石斛增加較大。YH-1和YQ-2多糖含量變化不顯著,而YH-1品種黃酮含量較YQ-2高。綜上所述,在UV-B照射下,不同濃度的茉莉酸甲酯在不同的時間內(nèi),對鐵皮石斛苗生長和主要活性成分的作用不一樣。高濃度的茉莉酸甲酯不利于鐵皮石斛苗的生長,而且使得鐵皮石斛的活性成分減少;茉莉酸甲酯的濃度適當降低,如UV-B+MeJA5,鐵皮石斛的多糖和黃酮含量較UV-B顯著提高,且有利于緩解UV-B輻射對鐵皮石斛苗生長的傷害作用;茉莉酸甲酯濃度過低,如UV-B+MeJA2.5,鐵皮石斛苗的多糖和黃酮含量則增加,但鐵皮石斛苗的生長受阻。因此當茉莉酸甲酯濃度為5 mmol/L(UV-B+MeJA5)時可以減輕UV-B照射對鐵皮石斛苗的傷害作用,并提高鐵皮石斛的總多糖和黃酮含量。同時研究發(fā)現(xiàn)茉莉酸甲酯對抗UV-B輻射存在時間效應。在6天內(nèi)添加茉莉酸甲酯能通過提高鐵皮石斛苗的光合色素含量、保護酶活性、主要活性成分,緩解UV-B輻射的傷害。在照射6天后,鐵皮石斛光合色素含量、保護酶活性、主要活性產(chǎn)物均降低。本實驗發(fā)現(xiàn)對于抗逆性不同的鐵皮石斛品種,其對光合色素含量、保護酶的影響不同�？鼓嫘栽綇姷谋Ｗo酶活性上升幅度越大,且膜脂過氧化程度較低。
[Abstract]:Our previous studies showed that UV-B radiation can affect the content of seedling growth of Dendrobium candidum and active ingredients. Methyl jasmonate in plants is stimulated by the outside signal molecules when the most rapid response, are involved in the process of plant growth and development in order to improve the adaptability of plants to stress. This paper discusses the UV-B radiation effects of Methyl Jasmonate on the growth of Dendrobium candidum and the main active ingredient, the experimental scheme and results are as follows: in this experiment, the plantlet of Dendrobium officinale as the research object, the radiation dose can cause UV-B damage of Dendrobium seedlings under (15.6 W cm-2), in MS medium, concentration of exogenous methyl jasmonate 5mmol/L. Then, last 3,6,9,12 hours UV-B irradiation. The influences of Dendrobium seedlings photosynthetic pigment content, antioxidant enzyme activity changes as well as the main active ingredient, and then select the concentration of methyl jasmonate UV-B can alleviate the radiation injury and improve the content of the active components of the appropriate time for 8 days, the periodic UV-B radiation experiment. The response of different concentration of Methyl Jasmonate on Dendrobium periodic UV-B irradiation. The experimental treatment, different concentration of methyl jasmonate immediately after spraying, 6 hours a day UV-B irradiation the concentration of methyl jasmonate, respectively: 0 mmol/L (UV-B), 1 mmol/L (UV-B+MeJA1, the same below), 2.5 mmol/L (UV-B+MeJA2.5), 5 mmol/L (UV-B+MeJA5), 7.5 mmol/L (UV-B+MeJA7.5), 10 mmol/L (UV-B+MeJA10) in the experiment. The first 2,4,6,8 days, respectively by the number of Dendrobium seedlings sprouting on the damage rate, leaf, photosynthetic pigment content, antioxidant enzyme activity changes as well as the main active ingredient, the study of Methyl Jasmonate on Dendrobium periodic UV-B radiation response duration and concentration. On this basis, to explore the UV-B radiation, Effect of methyl jasmonate has obvious difference of two resistance of Dendrobium species difference. The main results are as follows: (1) a short time under UV-B radiation of Methyl Jasmonate on Dendrobium candidum effect in UV-B continuous irradiation, Dendrobium seedlings photosynthetic pigment content decreased, while the addition of methyl jasmonate can improve the photosynthetic pigment content of.UV-B+MeJA and POD in group CAT was significantly higher than that in CK and UV-B group, the content of CK were increased by 40%, 55%, 55%, and the content of MDA was lower than that of CK, the content of total polysaccharide group increased significantly compared with CK and UV-B (P0.05). In the experiment after 3,6,9,12 hours of treatment the content is CK increased by 568%, 307%, 47%, 38%. with the extension of time, while in UV-B+MeJA group, the content of total flavonoids in 3 hours were lower than those of the CK increased gradually in 6 hours after the content in the experiment. After 6 hours treatment of UV-B+MeJA compared with CK significantly increased the content of chlorophyll a (P0.05), CK content was increased by 38 At the same time.2%. Dendrobium seedlings chlorophyll b, carotenoid content increased gradually. (2) the periodic UV-B irradiation effects of Methyl Jasmonate on Dendrobium UV-B under the irradiation of different concentration of Methyl Jasmonate on Dendrobium Candidium seedlings are not the same. With the increase of the concentration of jasmonic acid methyl ester, to UV-B radiation the blade damage mitigation capability decreased. In eighth days after irradiation, different concentrations of methyl jasmonate, on leaf damage rate were 100%, 87.9%, 51%, 84.4%, 83%, 100%. in the leaves of group UV-B+MeJA5 damage rate is minimum. In addition, UV-B+, MeJA1, UV-B+MeJA2.5, UV-B+MeJA5, UV-B+MeJA7.5 and UV-B+MeJA10 group from the early increase in number but, in the late sprouting and sprouting of UV-B+MeJA2.5 and the number of blocked; group UV-B+MeJA5 increased slowly. In 6 days before the experimental treatment, Dendrobium seedlings photosynthetic pigment content increased; in the 6 Reduce the days of Dendrobium seedlings photosynthetic pigment content. At the same time, different concentration of Methyl Jasmonate on Dendrobium seedlings photosynthetic pigment content and the effect of different UV-B+MeJA5, UV-B+MeJA7.5 group of photosynthetic pigment content is always higher than that of UV-B, UV-B+MeJA1, UV-B+MeJA2.5 and UV-B+MeJA10.UV-B+ MeJA5 group and UV-B+MeJA7.5 group in the activity of antioxidant enzymes increased significantly (P0.05 compared with UV-B UV-B+MeJA1, MDA). In addition, the content of UV-B+MeJA2.5 and UV-B+MeJA5 were lower than UV-B, UV-B+MeJA7.5 and UV-B+MeJA10 group MDA. High content of methyl jasmonate indicated that low concentration can reduce the UV-B radiation on membrane lipid peroxidation caused damage to a certain extent. The effect of the main active ingredients of Dendrobium candidum: on the fourth day the UV-B+MeJA5 and UV-B+MeJA7.5 group, the total polysaccharide content was higher than that of group UV-B (P0.05), the contents were 108% in group UV-B and 105%. in addition, in the experimental treatment No. 2,4,6 days, the total flavone content of UV-B+MeJA2.5 UV-B group was 86%, 106%, 41%. in the second day, the total flavonoids in the effect of UV-B+MeJA5 treatment was not significant (P0.05), in the first 2,6 days of total flavonoids were 100% for UV-B and 40%. (3) UV-B radiation, two varieties the Dendrobium seedlings under UV-B irradiation with methyl jasmonate response of two Dendrobium species of Exogenous Methyl jasmonate, photosynthetic pigment content increased. Dendrobium YQ-2 variety of photosynthetic pigment content, antioxidant enzyme activity than YH-1 species of Dendrobium officinale increased.YH-1 and YQ-2 change of polysaccharide content was not significant, and the YH-1 content of flavonoids varieties was higher than that of YQ-2. In conclusion, after UV-B irradiation, different concentrations of methyl jasmonate in different time on d.candidum seedling growth and the main active components of the role is not the same. The high concentration of methyl jasmonate to iron Dendrobium seedlings growth, and the active components of Dendrobium decreased; concentration of MeJA reduced, such as UV-B+MeJA5, polysaccharides and flavonoids content of Dendrobium candidum significantly increased compared with UV-B and UV-B, will help alleviate the radiation damage effect on the growth of Dendrobium seedlings; the concentration of methyl jasmonate is too low, such as UV-B+MeJA2.5, multi sugar and flavonoid content of Dendrobium seedlings increased, but the growth of Dendrobium seedlings blocked. So when MeJA concentration was 5 mmol/L (UV-B+MeJA5) can reduce harmful effects of UV-B irradiation on Dendrobium seedlings, and improve the Dendrobium polysaccharide and flavonoids contents. The study also found that methyl jasmonate against UV-B radiation has time effect. The addition of MeJA in 6 days can improve Dendrobium seedlings photosynthetic pigment content, protective enzyme activity, the main active ingredient, alleviate the radiation damage in UV-B. According to 6 days after the shooting, Dendrobium photosynthetic pigment content, protective enzyme activity, the main active products were decreased. The study found that for different resistance of Dendrobium varieties, the photosynthetic pigment content, protective enzyme activity of protective enzymes. The effects of different resistance stronger increase amplitude was bigger, and the membrane lipid peroxidation was low.

【學位授予單位】：廣東藥科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S567.239

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