本文關(guān)鍵詞： 盆栽小菊 花瓣 組培 激素 試管開花　出處：《寧夏大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本試驗(yàn)利用盆栽小菊花瓣作為外植體,建立以花瓣為外植體的高頻快繁再生系統(tǒng)。試驗(yàn)以MS作為基本培養(yǎng)基,添加生長素與細(xì)胞分裂素,通過正交試驗(yàn)方法篩選出愈傷組織誘導(dǎo)、不定芽分化以及繼代增殖中最優(yōu)激素配方。針對(duì)不定芽玻璃化現(xiàn)象采用提高滲透壓、減少細(xì)胞分裂素濃度、降低培養(yǎng)瓶內(nèi)空氣濕度進(jìn)行調(diào)控。通過離體培養(yǎng)盆栽小菊花芽進(jìn)行試管開花誘導(dǎo),通過單因子篩選影響因素,研究不同植物生長激素在植株試管開花中的作用。研究結(jié)果表明:(1)盆栽小菊 ' Chrystal Pink'品種愈傷分化的最適培養(yǎng)基配方為MS+NAA1.2mg/L+6-BA1.0mg/L+TDZ 0.4mg/L,誘導(dǎo)率為 74.56%。'Rainbow Worth' 品種為MS+NAA1.2mg/L+6-BA1.5mg/L+TDZ0.2mg/L,誘導(dǎo)率為 77.21%。'Chrystal Bronze' 品種為MS+NAA0.3mg/L+6-BA1.0mg/L+TDZ0.4mg/L,誘導(dǎo)率為 62.23%。' Little Rock ' 為MS+NAA1.2mg/L+6-BA1.0mg/L+TDZ0.4mg/L,誘導(dǎo)率為 28.87%。(2)盆栽小菊品種 'Chrystal Pink'、'Rainbow Worth'、'Chrystal Bronze' 最佳不定芽誘導(dǎo)培養(yǎng)基為 MS+6-BA1.0mg/L+NAA0.1mg/L,誘導(dǎo)率分別為 41.11%、43.33%、51.11%。品種'Little Rock'為 MS+6-BA1.0mg/L+NAA0.3mg/L+TDZlmg/L,誘導(dǎo)率為 22.22%。(3)四個(gè)品種盆栽小菊 'Chrystal Pink'、'Rainbow Worth'、'Chrystal Bronze'、'Little Rock'最佳繼代增殖培養(yǎng)基均為MS+6-BA0.6mg/L+NAA0.05mg/L+TDZ0.1mg/L。將盆栽小菊'Chrystal Pink,品種繼代增殖培養(yǎng)基中細(xì)胞分裂素含量減半,玻璃化率降低到21.11%。綜合玻璃化率與增值率兩方面因素來看,蔗糖濃度提升到50g/L有利于改善玻璃苗增殖。(4)盆栽小菊品種 'Chrystal Pink'、'Rainbow Worth'、'Chrystal Bronze' 最佳生根培養(yǎng)基為MS+NAA0.2。'Little Rock'品種的最佳生根培養(yǎng)基為MS培養(yǎng)基。(5)蔗糖濃度為50 g/L時(shí),開花率高,花期長。培養(yǎng)基中添加0.1mg/L的NAA開花率達(dá)到52.23%-65.56%。添加 0.1mg/L6-BA 開花率為 17.76%-28.89%。添加 0.5mg/LTDZ 誘導(dǎo)開花率為31.11%-37.78%。0.5mg/LKT對(duì)試管開花作用明顯,開花率達(dá)到39.89%-43.23%。在MS培養(yǎng)基中添加1.0mg/LGA3時(shí)開花率達(dá)到39.89%-43.33%。
[Abstract]:In this experiment, the potted chrysanthemum flaps were used as explants to establish a high frequency rapid propagation regeneration system with petals as explants. Ms was used as the basic medium and auxin and cytokinin were added. The optimal hormone formula for callus induction, adventitious bud differentiation and subculture proliferation was obtained by orthogonal experiment. The vitrification of adventitious buds was treated by increasing the osmotic pressure and reducing the concentration of cytokinins. Through in vitro culture pot culture of chrysanthemum flower bud for tube flowering induction, through the single factor screening influencing factors, The effect of different plant growth hormone on plant in vitro flowering was studied. The results showed that the optimal medium for callus differentiation of potted chrysanthemum 'Chrystal' was MS NAA1.2mg/L 6-BA 1.0 mg / L TDZ 0.4 mg / L, and the induction rate was 74.56.Rainbow Worth' was MS NAA1.2mg/L 6-BA 1.5 mg / L. The inductive rate of MS NAA0.3mg/L 6-BA1.0 mg / L TDZ0.4 mg / L, and the inductive rate of MS NAA1.2mg/L 6-BA1.0 mg / L TDZ0.4 mg / L, 'Little Rock' was MS 6-BA1.0 mg / L TDZ0.4 mg / L, and the inducement rate was 28.877.82) the best adventitious bud induction medium was MS 6-BA1.0 mg / L NAA 0.1 mg / L, and the inducement rate was 41.11% 43.33 mg / L, respectively, in the medium of MS 6-BA1.0 mg / L NAA0.1 mg / L, and the inductive rate was 41.11 mg / L, 43.33 mg / L and 41.11% / L, respectively, and the optimal adventitious bud induction medium was MS 6-BA1.0 mg / L NAA0.1 mg / L, and the inductive rate was 28.87 mg / L, and the optimal medium for inducing adventitious buds was MS 6-BA1.0 mg / L NAA 0.1 mg / L, and the induced rate was 41.11% 43.33 mg / L, respectively. The best subculture medium for MS 6-BA 1.0 mg / L NAA0.3mg/L TDZlmg / L, inductive rate 22.222.35) is MS 6-BA 0.6 mg / L NAA0.05mg/L TDZ0.1 mg / L, and the content of cytokinin in the subculture medium is halved, and the content of cytokinin in the subculture medium is halved, and the content of cytokinin is halved in the subculture medium of Chrystal Pinkin, the best subculture medium is MS 6-BA 0.6 mg / L NAA0.05mg/L TDZ0.1 mg / L, and the content of cytokinin in the subculture medium is halved. The vitrification rate is reduced to 21.11. Considering both the vitrification rate and the value added rate, Raising sucrose concentration to 50g / L could improve the proliferation of glass-seedling. 4) the best rooting medium for MS NAA0.2.'Little Rock' was MS medium. The best rooting medium for MS NAA0.2.'Little Rock' was MS medium with 50 g / L sucrose concentration, and the flowering rate was high when sucrose concentration was 50 g / L. The best rooting medium was Chrystal Bronze' for potted chrysanthemum, and the best rooting medium for MS NAA0.2.'Little Rock' was 50 g / L of sucrose. The flowering rate of NAA with 0.1 mg / L was 52.23-65.56. The flowering rate of 0.1 mg / L 6-BA was 17.76-28.89. The induced flowering rate was 31.11-37.78.0.5mg / L LKT had obvious effect on tube flowering. The flowering rate reached 39.89- 43.23.The addition of 1.0 mg / L 6-BA in MS medium reached 39.89- 43.33.
【學(xué)位授予單位】：寧夏大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S682.11

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