本文關(guān)鍵詞：中國蚜小蜂科亞科和族的鑒別及基于ITS2序列的黃蚜小蜂屬系統(tǒng)發(fā)育研究　出處：《福建農(nóng)林大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 蚜小蜂科 支序分析 黃蚜小蜂屬 ITS2 rDNA 系統(tǒng)發(fā)育分析

【摘要】：蚜小蜂科Aphelinidae隸屬膜翅目Hymenoptera,小蜂總科Chalcidoidea,是重要的天敵昆蟲,在害蟲生物防治中具重要經(jīng)濟意義。目前該科部分分類單元的劃分還比較混亂,進行深入的系統(tǒng)分類和系統(tǒng)發(fā)育研究十分必要。本論文基于蚜小蜂科的63個外部形態(tài)特征,其中包括1個整體特征、14個頭部特征、19個胸部特征、5個腹部特征、19個翅特征和5個足特征,以跳小蜂科的3種Thomsonisca typica、Anabrolepis bifasciata和Coccidencyrtus tp.為外群,采用支序法,對中國已發(fā)表的該科22屬中的55種進行系統(tǒng)發(fā)育分析,并在此基礎(chǔ)上進行亞科和族的劃分;另外,本論文還對17種黃蚜小蜂進行了 ITS2的測定,并從NCBI上下載黃蚜小蜂屬的17條ITS2 rDNA基因序列,以Eretmocerus屬為外群,分別采用鄰接法(NJ)、最大簡約法(MP)和貝葉斯法(BI)構(gòu)建系統(tǒng)發(fā)育樹,進行系統(tǒng)發(fā)育研究。結(jié)論如下:1.本研究采用Hayat(1998)的分類系統(tǒng)支持將中國蚜小蜂科分為 Aphelininae亞科 Eriaphytinae 亞科、Azotinae亞科 Coccophaginae亞科、Euryischiinae 亞科和 Eriaporinae 亞科,其中,Aphelininae 亞科分為 Aphytini 族(Aphytis屬、Centrodora 屬、Marietta屬、Proaphelinoides屬和Marlattiella 屬)、Eretmocerini族(Eretmocerus屬)和 Aphelini 族(Aphelinus屬和 Protaphelinus 屬)3 個族;Eriaphytinae亞科、Azotinae 亞科和 Euryischiinae 亞科為單型屬;Coccophaginae亞科分為 Coccophagini族(Coccophag s屬和Prococcophagu 屬)、Physcini 族(Coccobius 屬 Pteroptricini 族(Pteroptrix 屬、Archenomus屬、Aphelosoma 屬、Encarsia 屬、Coccophagoides 屬和 Prococcophagus屬)3個族。2.Aphelininae 亞科、Azotinae 亞科、Euryischiinae 亞科、Eriaphytinae亞科和Eriaporinae亞科都有較高的支持度,為單系群;Coccophaginae亞科是復(fù)系群。Aphelininae亞科進化最快,在該科中Eretmocerini族與Marlattiella屬進化關(guān)系較近;Aphytini族是復(fù)系群,Aphytis屬最進化;Aphelinini族是單系群,在Aphelininae亞科中最為原始。Coccophaginae 亞科為復(fù)系群,Pteroptricini 族較 Coccophagini族和Physcini族進化;Physcini族是單系群;Pteroptricini族和Coccophagini族是復(fù)系群,建議恢復(fù)Coccophagini族的Prococcophagus屬的屬級地位。3.本研究對17種黃蚜小蜂進行了 ITS2 rDNA的測定,得到105條序列,由序列分析可知,其A+T的平均含量為52.6%,高于G+C的平均含量(47.4%),轉(zhuǎn)換的頻率是顛換頻率的0.67倍,序列變異性很強。用MEGA 6.05軟件分別計算17種黃蚜小蜂的ITS2 rDNA基因序列的種內(nèi)、種間遺傳距離,結(jié)果得出種內(nèi)、種間平均遺傳距離分別是0～7.8%和0.523;其中,A nr diaspidis與Aphytis sp.2之間的遺傳距離最小為 0.023,A.holoxanthus、A.nr holoxanthus、A.melinus、Aphytis sp.1之間的遺傳距也相對較小;Aphytissp.12和funicularis種團的遺傳距離最大為0.889,其他種之間親緣關(guān)系較遠的還有funicularis種團與Aphytis sp.4和Aphy is sp.3。4.分析NJ、MP和BI三種方法所建系統(tǒng)發(fā)育樹的關(guān)系,結(jié)論如下:BI樹最好的呈現(xiàn)了黃蚜小蜂屬的系統(tǒng)發(fā)育關(guān)系,其中,lingnanensis種團為單系群,分為A.lingnanensis和Aphytissp.1+(AphytiS melinus+(A.holoxcanthus+(4.nr holoxanthus)))兩個亞種團;chrysomphali種團和proclia種團都不是單系群,Aphytis sp.5與lingnanensis種團的lingnanensis亞種團親緣關(guān)系較近,A nr crysomphali·與Aphytis sp.6是姐妹群;Z145與A.nr diaspidis是同一種;funicularis種團為單系群,最原始。
[Abstract]:Aphelinidae Aphelinidae Hymenoptera Chalcidoidea belonging to Hymenoptera, Chalcidoidea, is an important natural enemy insects, with important economic significance in biological control of insect pests. The division of the Department of some taxa also chaotic, the classification system and in-depth research and development is very necessary. This paper Aphelinidae 63 morphological characteristics based on the overall features including 1, 14 head features, 19 chest features, 5 abdominal features, 19 features and 5 foot wing features in 3 Thomsonisca typica Encyrtidae, Anabrolepis bifasciata and Coccidencyrtus tp. as outgroup, the cladistic method of China published the 22 genera in 55 kinds of phylogenetic analysis, and on the basis of dividing subfamily and family; in addition, this paper also carried out ITS2 determination of 17 species of the aphelinid, and download the Yellow Encarsia from NCBI 17 ITS2 rDNA gene sequences of the genus to genus Eretmocerus as outgroup, respectively using neighbor joining (NJ), maximum parsimony (MP) and Bayesian method (BI) to construct phylogenetic tree for phylogenetic studies. The conclusions are as follows: 1. the research by Hayat (1998) classification system will support China aphid EUPELMIDAE divided into Aphelininae subfamily of Eriaphytinae subfamily, Azotinae subfamily of Coccophaginae subfamily, Euryischiinae subfamily and Eriaporinae subfamily, the Aphelininae subfamily is divided into Aphytini group (Aphytis, Centrodora, Marietta, Proaphelinoides and Marlattiella genus), Eretmocerini group (Eretmocerus) and Aphelini group (Aphelinus and Protaphelinus genus 3 family); Eriaphytinae subfamily, Azotinae subfamily and Euryischiinae subfamily is a monotypic genus; Coccophaginae subfamily is divided into Coccophagini group (Coccophag s and Prococcophagu genus), Physcini Family (genus Coccobius of Pteroptricini family (genus Pteroptrix, genus Archenomus, genus Aphelosoma, genus Encarsia, genus Coccophagoides and genus Prococcophagus) 3 family.2.Aphelininae subfamily, Azotinae subfamily, Euryischiinae subfamily, Eriaphytinae subfamily and Eriaporinae subfamily have a high degree of support, is a monophyletic group; Coccophaginae subfamily is polyphyly.Aphelininae subfamily evolution the fastest in the family of Eretmocerini and Marlattiella is a close evolutionary relationship; group Aphytini is polyphyletic group, Aphytis is the evolution; Aphelinini group is monophyletic, in Aphelininae subfamily the most primitive subfamily.Coccophaginae is polyphyletic group, Pteroptricini group compared with Coccophagini group and Physcini group Physcini group is single evolution; Department of the group; Pteroptricini group and Coccophagini group is polyphyletic group, Coccophagini group and Prococcophagus of the genus of.3. status in 17 recovery proposals Yellow aphid wasps were determined by ITS2 rDNA, obtained 105 sequences, from the analysis of sequence, the average content of A+T is 52.6%, the average content is higher than that of G+C (47.4%), the switching frequency is 0.67 times the frequency of transversion, sequence variability is very strong. The ITS2 rDNA gene sequence to calculate the 17 kinds of yellow aphid wasp respectively with MEGA 6.05 software within species, the genetic distance between species, the species, the average genetic distance is 0 ~ 7.8% and 0.523 respectively; the NR diaspidis Aphytis SP.2 between A and the smallest genetic distance was 0.023, A.holoxanthus, A.nr holoxanthus, A.melinus Aphytis sp.1, the genetic distance between it relatively small; Aphytissp.12 and funicularis group the largest genetic distance was 0.889. The genetic relationship between other distant and funicularis group with Aphytis sp.4 and Aphy is sp.3.4. analysis of NJ, MP and BI system built by the three methods of phylogenetic tree. The conclusions are as follows: BI tree, and present the best system of aphytis development, among them, the lingnanensis group is a monophyletic group, divided into A.lingnanensis and Aphytissp.1+ (AphytiS melinus+ (A.holoxcanthus+ (4.nr holoxanthus))) two subspecies group; chrysomphali group and proclia group are not monophyletic. Close to Aphytis sp.5 and the lingnanensis group lingnanensis group A NR subspecies relationship, crysomphali and Aphytis sp.6 are sister groups; Z145 and A.nr diaspidis are the same; the funicularis group is monophyletic, the most primitive.

【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S476.3

【參考文獻】

相關(guān)期刊論文 前10條

1 張毅波;楊念婉;張桂芬;王進軍;萬方浩;;利用COⅠ基因序列和雜交實驗分析鑒定槳角蚜小蜂屬的本地種群[J];環(huán)境昆蟲學(xué)報;2015年02期

2 王竹紅;黃建;李成德;;蚜小蜂科一中國新紀(jì)錄屬——原蚜小蜂屬記述(膜翅目:小蜂總科)(英文)[J];Entomotaxonomia;2014年03期

3 周青松;張同心;于芳;羅阿蓉;牛澤清;朱朝東;張彥周;;DNA條形碼在膜翅目昆蟲中的應(yīng)用分析[J];應(yīng)用昆蟲學(xué)報;2013年01期

4 阮用穎;王竹紅;黃建;;中國蚜小蜂科一新紀(jì)錄屬及一新紀(jì)錄種(膜翅目,小蜂總科)[J];動物分類學(xué)報;2012年02期

5 黃華平;楊臘英;王國芬;黃俊生;;rDNA和mtDNA在昆蟲系統(tǒng)發(fā)育與區(qū)系研究中的應(yīng)用[J];華南熱帶農(nóng)業(yè)大學(xué)學(xué)報;2006年04期

6 李成德,張爽;中國蚜小蜂屬一新種及一新記錄種(膜翅目:蚜小蜂科)[J];昆蟲分類學(xué)報;2005年01期

7 耿金虎,李正西,沈佐銳;診斷引物應(yīng)用于我國三種重要赤眼蜂分子鑒定的研究[J];昆蟲學(xué)報;2004年05期

8 王竹紅,黃建,梁智生,連炳賢,林慶源,鐘景輝;松突圓蚧花角蚜小蜂的引種與利用[J];福建農(nóng)業(yè)大學(xué)學(xué)報;2004年03期

9 黃大衛(wèi);新世紀(jì)的中國昆蟲系統(tǒng)學(xué)[J];昆蟲學(xué)報;2003年01期

10 龔志云,吳信淦,程祝寬,顧銘洪;水稻45S rDNA和5S rDNA的染色體定位研究[J];遺傳學(xué)報;2002年03期

相關(guān)碩士學(xué)位論文 前10條

1 胡澤章;幾種蚜小蜂的分子鑒定與麗蚜小蜂線粒體基因片段的研究[D];福建農(nóng)林大學(xué);2015年

2 尚亞飛;側(cè)跗葉蜂屬分子系統(tǒng)研究[D];中南林業(yè)科技大學(xué);2014年

3 李艷華;槳角蚜小蜂屬和食蚧蚜小蜂屬部分種類基因序列及系統(tǒng)發(fā)育關(guān)系研究[D];福建農(nóng)林大學(xué);2014年

4 張志敏;恩蚜小蜂屬部分種類DNA條形碼的構(gòu)建及系統(tǒng)發(fā)育研究[D];福建農(nóng)林大學(xué);2014年

5 劉宏泉;新疆地區(qū)蚜小蜂科系統(tǒng)分類研究（膜翅目：小蜂總科）[D];新疆農(nóng)業(yè)大學(xué);2013年

6 耿淑影;中國蚜小蜂屬系統(tǒng)分類研究（膜翅目：蚜小蜂科）[D];東北林業(yè)大學(xué);2011年

7 孫莉;新疆、西藏部分地區(qū)蚜小蜂資源的初步調(diào)查與分類研究（膜翅目：小蜂總科）[D];福建農(nóng)林大學(xué);2010年

8 劉婷;垂葉榕非傳粉小蜂Walkerella屬兩個種及其雄性類型的形態(tài)和分子學(xué)鑒別[D];山東農(nóng)業(yè)大學(xué);2008年

9 楊郁霞;中國小蜂科高級階元系統(tǒng)發(fā)育關(guān)系及Lucid檢索表[D];福建農(nóng)林大學(xué);2005年

10 楊慶寅;中國東北地區(qū)及韓國黃蚜小蜂屬的分類研究（膜翅目：蚜小蜂科）[D];東北林業(yè)大學(xué);2003年

，

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