本文關(guān)鍵詞：固態(tài)發(fā)酵豆粕制備氨基酸復(fù)合肥的工藝研究　出處：《中國海洋大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 豆粕 固態(tài)發(fā)酵 氨基酸螯合中微量元素 葉面肥 大田試驗

【摘要】：氨基酸復(fù)合肥是以大量元素原料與以氨基酸為配體與中微量元素金屬離子絡(luò)合形成的螯合物原料復(fù)配得到的一種全元素復(fù)合肥料。氨基酸中微量元素螯合物化學(xué)及生化穩(wěn)定性高,擁有很好的配伍性。本文以豆粕為蛋白原料,利用實驗室保存的產(chǎn)蛋白酶菌種WY-3,通過固態(tài)發(fā)酵的方式對豆粕進(jìn)行降解,水提后得到氨基酸溶液。在適宜條件下將氨基酸與中微量元素金屬離子進(jìn)行水系復(fù)合螯合,得到氨基酸中微量元素螯合物。按照不同作物的營養(yǎng)需求進(jìn)行科學(xué)復(fù)配,制備出全元素的氨基酸螯合葉面肥,并進(jìn)行大田試驗,檢驗肥效。本文通過單因素實驗、響應(yīng)面優(yōu)化等統(tǒng)計學(xué)分析方法,先對固態(tài)發(fā)酵降解豆粕蛋白質(zhì)的可行性及最優(yōu)條件進(jìn)行了分析研究,實驗結(jié)果顯示實驗室保存的產(chǎn)蛋白酶菌種WY-3可對豆粕進(jìn)行良好的降解。通過優(yōu)化分析,結(jié)果證明在pH為6.5-7.0,料液比為1：0.7-1：0.8,溫度為32℃,接種量為5%的條件下,發(fā)酵9-16天豆粕蛋白降解度達(dá)到最大,蛋白溶出率高達(dá)95%以上。通過對固態(tài)發(fā)酵工藝進(jìn)一步放大設(shè)計,找到了一條成本低廉、成熟穩(wěn)定的工藝路線。以25L塑料圓桶發(fā)酵方式為例,通過實驗發(fā)現(xiàn)料層厚度、接種量、料液比是對發(fā)酵結(jié)果影響最為重要的三個因素。通過優(yōu)化發(fā)現(xiàn),當(dāng)接種量為10%,料層厚度為15cm,水：豆粕=0.8：1,pH保持自然狀態(tài)下時,在室溫下發(fā)酵6天的條件可實現(xiàn)發(fā)酵產(chǎn)物蛋白溶出率80%以上的效果。在發(fā)酵完成后,采用三足刮刀式離心機(jī)離心、旋蒸濃縮等工藝可將水溶物進(jìn)行大規(guī)模提取和濃縮,得到螯合所用的配體氨基酸原料。以肥料當(dāng)中常用的中微量元素金屬鹽為原料,通過三步螫合法,即復(fù)合螯合、單獨螯合、最后復(fù)配等過程很好的解決了各元素之間的配伍性問題。通過對不同作物的營養(yǎng)需求進(jìn)行研究、結(jié)合實際種植試驗制定出合適的全元素配方,按照配方比例進(jìn)行復(fù)配,制備出一種全水溶性的氨基酸螯合中微量元素復(fù)合葉面肥。通過大田試驗檢驗肥效,其增產(chǎn)效果明顯,并利用發(fā)酵廢棄物制備得到一種有機(jī)型沖施復(fù)合肥。
[Abstract]:Amino acid compound fertilizer is a kind of all-element compound fertilizer which is formed by chelate material complexing with amino acid as ligand and middle trace element metal ion. Chemistry of trace element chelate in amino acid. And high biochemical stability. In this paper, soybean meal was used as protein raw material, and the protease producing strain WY-3 was used to degrade soybean meal by solid-state fermentation. After water extraction, amino acid solution was obtained. Under suitable conditions, amino acids were chelated with metal ions in water system. The chelate of trace elements in amino acids was obtained. According to the nutritional requirements of different crops, the amino acid chelate foliar fertilizer of all elements was prepared, and the field experiment was carried out. Through single factor experiment, response surface optimization and other statistical analysis methods, the feasibility and optimal conditions of solid-state fermentation for protein degradation of soybean meal were studied. The results showed that the protease producing strain WY-3 could degrade soybean meal well. The results showed that the pH was 6.5-7.0. When the ratio of material to liquid was 1: 0.7-1: 0.8, the temperature was 32 鈩，

本文編號：1399753