本文關(guān)鍵詞：妊娠后期營(yíng)養(yǎng)限制對(duì)蒙古綿羊肝臟脂類代謝、抗氧化能力和急性期蛋白合成的影響　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 妊娠后期 營(yíng)養(yǎng)限制 蒙古綿羊肝臟 脂類代謝 抗氧化 急性期蛋白

【摘要】：本試驗(yàn)旨在研究妊娠后期營(yíng)養(yǎng)限制對(duì)蒙古綿羊肝臟脂類代謝、抗氧化能力及急性期蛋白合成的影響。選擇2～3胎次,體況中等,體重相近,經(jīng)同期發(fā)情受孕,健康的蘇尼特蒙古綿羊24只進(jìn)行試驗(yàn)。在妊娠90d時(shí),選擇6只接近總體均重的母羊進(jìn)行屠宰,其余的羊只采取隨機(jī)原則分配到三個(gè)營(yíng)養(yǎng)水平處理組,即CG組(自由采食組)、RG2組(限制組2)(0.33MJME.kgw-0.75.d-1),RG1組(限制組1)(0.175 MJME.kgw-0.75.d-1),飼養(yǎng)至妊娠140d進(jìn)行屠宰。結(jié)果表明:1.妊娠后期隨著營(yíng)養(yǎng)水平的降低,RG1、RG2組母羊凈重(P0.01)、肝臟重(P0.05)、蛋白質(zhì)含量(P0.05)顯著低于CG組;RG1組母羊肝臟干物質(zhì)量、脂肪含量顯著高于CG、RG2組(P0.01),且RG2組母羊肝臟干物質(zhì)量顯著低于CG組(P0.05),RG2組母羊肝臟脂肪含量低于CG組,但兩組間沒(méi)有顯著差異;母羊肝臟粗灰分含量呈下降趨勢(shì),但三組間沒(méi)有顯著差異;RG1組母羊肝臟NO含量和NOS活性顯著高于CG組(P0.05);CG組母羊肝臟CHE(膽堿酯酶)和AKP(堿性磷酸酶)的活性顯著高于RG1組(P0.05)。2.妊娠后期隨著營(yíng)養(yǎng)水平的降低,RG1組母羊肝臟TG(甘油三酯)含量顯著高于CG、RG2組(P0.01);RG1組母羊肝臟TC(總膽固醇)含量顯著低于CG、RG2組(P0.01),但CG、RG2組間沒(méi)有顯著差異;RG2、RG1組母羊血液中NEFA(游離脂肪酸)含量顯著高于CG組(P0.01);RG1組母羊血液BHBA(β-羥丁酸)含量顯著高于RG2、CG組(P0.01);RG1組母羊肝臟LPL(脂蛋白酯酶)和HL(肝脂酶)活性顯著低于CG組(P0.01),RG2組母羊肝臟LPL(脂蛋白酯酶)(P0.01)和HL(肝脂酶)(P0.05)活性顯著低于CG組。3.妊娠后期隨著營(yíng)養(yǎng)水平的降低,母羊肝臟T-AOC(總抗氧化能力),SOD(超氧化物歧化酶),GSH-PX(谷胱甘肽過(guò)氧化物酶),MDA(丙二醛)含量呈升高趨勢(shì),RG1 組 T-AOC(P0.05)、SOD(P0.01)、GSH-PX(P0.01)、MDA(P0.01)顯著高于RG2、CG組。4.妊娠后期隨著營(yíng)養(yǎng)水平的降低,母羊肝臟HPT(結(jié)合珠蛋白)和CP(銅藍(lán)蛋白)的含量逐漸升高,但三組間差異不顯著:母羊肝臟CRP(C反應(yīng)蛋白)含量逐漸降低,RG1組母羊肝臟CRP(C反應(yīng)蛋白)的含量顯著低于CG組(P0.05),RG2組母羊肝臟CRP(C反應(yīng)蛋白)含量與CG、RG1組均沒(méi)有顯著差異;母羊血液CG組HPT(結(jié)合珠蛋白)、CP(銅藍(lán)蛋白)含量顯著高于RG2、RG1組(P0.05),CRP(C反應(yīng)蛋白)各組間差異不顯著,但RG2組母羊血液CRP(C反應(yīng)蛋白)含量高于CG組,低于RG1組。從以上結(jié)果可知,由于妊娠后期營(yíng)養(yǎng)限制RG1組母羊動(dòng)員體脂,母羊肝臟發(fā)生了脂肪肝和妊娠毒血癥,發(fā)生氧化應(yīng)激,導(dǎo)致肝功能受損,改變了肝細(xì)胞合成急性期蛋白的能力;而RG2組營(yíng)養(yǎng)限制水平,母羊雖然動(dòng)員了體脂,但沒(méi)有超出母羊肝臟的適應(yīng)性調(diào)節(jié)能力范圍,母羊沒(méi)有發(fā)生脂肪肝和妊娠毒血癥,肝功能也沒(méi)有顯著受損。
[Abstract]:This experiment was conducted to study the late pregnancy nutrition restriction on the metabolism of Mongolia sheep liver lipids, antioxidant ability and effect of acute phase protein synthesis. 2 ~ 3 parity, body condition medium, similar weight, the estrus conception, the health of Mongolia Sunite 24 sheep were tested. In pregnancy 90d, 6 were close to overall the ewes were slaughtered, the rest of the sheep were randomly assigned to take three nutrition treatment group, CG group (ad libitum group), group RG2 (restriction group 2) (0.33MJME.kgw-0.75.d-1), RG1 group (Group Limited 1) (0.175 MJME.kgw-0.75.d-1), feeding to slaughter. The results showed that 140d of pregnancy 1.: late pregnancy with lower nutrient levels of RG1, RG2 group (P0.01), ewe weight and liver weight (P0.05), protein content (P0.05) was significantly lower than that of CG group; the dry weight of group RG1 ewes liver fat content was significantly higher than that of CG, RG2 group and RG2 group (P0.01), mother 緹婅倽鑴忓共鐗╄川閲忔樉钁椾綆浜嶤G緇，

本文編號(hào)：1385097