本文關(guān)鍵詞：硅緩解歐美楊無性系I-214鎘脅迫的生理及轉(zhuǎn)錄調(diào)控機理　出處：《沈陽農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 硅 鎘 I-214楊 生理響應(yīng) 轉(zhuǎn)錄組測序

【摘要】：當前鎘(Cd)污染嚴重影響著農(nóng)業(yè)生態(tài)環(huán)境和可持續(xù)發(fā)展。植物修復(fù)是Cd污染土壤修復(fù)的重要潛在技術(shù)。硅(Si)是植物的有益元素,能增強植物抗逆性,在植物修復(fù)中具有廣泛的應(yīng)用前景。本文以歐美楊無性系1-214為研究對象,采用Hoagland營養(yǎng)液水培方式,研究Si對Cd脅迫下1-214楊的生理和轉(zhuǎn)錄測序,以揭示施加Si緩解1-214楊Cd毒害的機理。主要結(jié)論如下:(1)Si通過改善1-214楊的根系活力、加強1-214楊根系對水分和養(yǎng)分的吸收。并通過降低活性氧的生成和脂質(zhì)過氧化程度,增加可溶性蛋白與Cd的結(jié)合和莖部對Cd的吸收來緩解Cd對1-214楊幼苗的毒害作用。(2)非酶抗氧化系統(tǒng)可能不是Si緩解1-214楊幼苗Cd脅迫的主要途徑。(3)以pval0.05且Fold change ≥ 2為差異基因的篩選標準。Cd處理組與對照組相比,共有146個差異表達基因,其中86個上調(diào)基因,60個下調(diào)基因。Cd+Si處理組與Cd處理組相比,共有540個差異表達基因,其中214個上調(diào)基因,326個下調(diào)基因。(4)差異表達基因GO和KEGG富集分析得出:Cd處理組與對照組相比,主要涉及熱休克蛋白、小熱激蛋白(HSP20)、細胞色素P450、硫氧還蛋白;Cd+Si處理組與Cd處理組相比,主要涉及轉(zhuǎn)錄因子WRKY、GRAS、DELLA、蛋白激酶以及鈣結(jié)合的EF手結(jié)構(gòu)。實時定量熒光PCR驗證結(jié)果與測序結(jié)果中基因變化趨勢基本一致。(5)以對照組SNP和InDel事件發(fā)生數(shù)量為參照,Cd+Si處理組比Cd處理組的SNP事件數(shù)量升高2.45%;InDel事件數(shù)量升高3.08%。同時新轉(zhuǎn)錄本預(yù)測中新外顯子區(qū)域占了 94.99%。Si可能通過提高SNPs和InDels事件的發(fā)生率以及誘導(dǎo)新外顯子區(qū)域的形成來緩解Cd脅迫對I-214楊正常生長的抑制作用。
[Abstract]:At present, cadmium and cadmium (CD) pollution seriously affect the agricultural ecological environment and sustainable development. Phytoremediation is an important potential technology for CD contaminated soil remediation. SiSi) is a beneficial element of plants. It can enhance plant resistance and has a wide application prospect in phytoremediation. In this paper, the clone 1-214 of American and European poplar was used as the research object, and the method of Hoagland nutrient solution hydroculture was adopted in this paper. The physiological and transcriptional sequencing of poplar 1-214 under CD stress was studied. In order to reveal the mechanism of applying Si to alleviate CD toxicity of 1-214 poplar, the main conclusions are as follows: 1: 1 Si can improve the root activity of 1-214 poplar. The absorption of water and nutrients by the roots of 1-214 poplar was strengthened, and the production of active oxygen and the degree of lipid peroxidation were decreased. To alleviate the toxicity of CD to 1-214 poplar seedlings by increasing the binding of soluble protein to CD and the absorption of CD by stem. Non-enzymatic antioxidant system may not be the main way for Si to alleviate CD stress in poplar seedlings 1-214. Pval0.05 and Fold change 鈮，

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