患有肌肉骨骼系統(tǒng)疾病和骨組織損傷的病人數(shù)量在逐年增加。在美國,占外傷病人總數(shù)的三分之二,每年約有630萬人骨折,超過50萬人需要進(jìn)行骨移植。創(chuàng)傷性損傷造成的骨組織大量丟失為骨組織重建帶來了巨大的臨床挑戰(zhàn)。在這些損傷中,嚴(yán)重的骨缺損特別難以修復(fù),通常需要二次手術(shù),最終導(dǎo)致骨組織不愈合。目前對于復(fù)雜骨折治療常用的方法為利用自體髂嵴骨骺游離移植進(jìn)行重建。但是該技術(shù)受限于可用于自體髂嵴骨骺數(shù)量有限,且會給患者本身帶來巨大的疼痛進(jìn)而造成愈合的難度。異體移植也常用于骨組織損傷治療,但移植的條件相對于自體移植來說失敗的風(fēng)險隨著患病時間也顯著升高,并伴隨著疾病傳播的風(fēng)險。另外,異種移植物在移植前必須經(jīng)過滅菌過程,會導(dǎo)致多種骨誘導(dǎo)因子在移植材料內(nèi)的失活,進(jìn)而喪失功能。同時為了提高骨愈合,髓樣棒和用于內(nèi)部固定的金屬設(shè)備有時也是必須的。然而,這些永久性的植入物由于是金屬故不會被患者吸收融合,并且在長期使用期間容易發(fā)生疲勞性骨折,將這種金屬器件與相鄰的骨頭結(jié)合是極具挑戰(zhàn)性的。目前研究結(jié)果顯示,利用生物陶瓷以及在植入物表面進(jìn)行礦化涂層可以顯著的提高與組織的相容性,但這些表面調(diào)節(jié)技術(shù)會導(dǎo)致植入裝置的耐久性的下降。... 

【文章來源】：哈爾濱工業(yè)大學(xué)黑龍江省 211工程院校 985工程院校

【文章頁數(shù)】：103 頁

【學(xué)位級別】：博士

【文章目錄】：
Abstract (In Chinese)
Abstract (In English)
Chapter 1 Introduction
    1.1 Background and significance of the subject
    1.2 Description of bone fracture healing process
    1.3 Development of EIS
        1.3.1 Theory of conducting EIS measurements of living tissues
        1.3.2 EIS Characteristics of Bone Tissue and Current Applications of EIS in the Study of Bone Tissue
    1.4 Research contents and technical route of this subject
        1.4.1 Main research contents
        1.4.2 Technical route
Chapter 2 Materials and methods
    2.1 Materials
        2.1.1 Animals and cell lines
        2.1.2 Regents and equipments
        2.1.3 Web site and software
    2.2 Methods
        2.2.1 Apparatus for electric impedance measurements
        2.2.2 Electrical stimulation
        2.2.3 Preparation of scanning electron microscope samples
        2.2.4 Immunofluorescence
        2.2.5 X-ray investigation technique
        2.2.6 Gross anatomy and histologic analyses
        2.2.7 Replication the bone healing process in vitro
        2.2.8 Creation of 3D Cell Culture S ystems
        2.2.9 Surgical procedure for creation rabbit bone CSD
        2.2.10 Statistical analysis
Chapter 3 The investigation of bone healing process with EIS using in vitromodel
    3.1 Introduction
    3.2 Investigation of in vitro bone healing process
        3.2.1 Investigation of the 3D models with different amount of collagen
        3.2.2 Investigation of the 3D models with different amount of HA
        3.2.3 Investigation of the 3D models with different amount of HAp
    3.3 Brief summary
Chapter 4 A dual-transduction-integrated biosensing system to examine the 3D cell culture for bone regeneration
    4.1 Introduction
    4.2 Investigation of bone-like 3D cell culture systems
        4.2.1 3D culture systems without cells and their characterization
        4.2.2 3D cell culture systems with living cells and their characterization
        4.2.3 Investigation of electrical stimulation of 3D cell culture system
    4.3 Brief summary
Chapter 5 The investigation of bone healing process with EIS using rabbitmodel
    5.1 Introduction
    5.2 Investigation in vivo bone healing process
        5.2.1 In vivo measurement of the electric impedance of bone healing
        5.2.2 Radiological findings
        5.2.3 Gross anatomy and histologic analyses
    5.3 Brief summary
Conclusions
References
Appendix
Papers published in the period of Ph.D. education
Acknowledgements
Resume



本文編號：3296327