人參皂苷Rg3抑制人骨肉瘤細胞增殖及其分子機制研究
發(fā)布時間：2021-07-10 17:14
研究背景:骨肉瘤是發(fā)源于間充質干細胞的惡性腫瘤。骨肉瘤的發(fā)病年齡呈現(xiàn)雙峰分布,常發(fā)病于青少年或老年人。由于大多數(shù)的骨肉瘤患者在診斷時時就有轉移或微轉移性,故幾乎所有患者在手術切除的之前或之后都會接受多種藥物聯(lián)合化療。患者往往耐受度較差,且復發(fā)幾率較高。人參是一種原產于東北亞的草本植物,在東亞地區(qū)的傳統(tǒng)醫(yī)學中有著廣泛的應用。作為人參中主要的活性物質之一,人參皂苷Rg3的抗腫瘤作用在近年來越來越引起人們的重視。人參皂苷Rg3的甾體結構使得它可以與細胞膜、膜結合離子通道、以及胞內和胞外的受體相互作用來改變細胞的轉錄水平以來發(fā)揮抗腫瘤作用。研究方法:本實驗應用MTT實驗、流式細胞術、細胞劃痕實驗、Transwell細胞侵襲實驗檢測了人參皂苷Rg3對骨肉瘤細胞系MG-63、HOS和U2OS增殖、凋亡、遷移、侵襲的影響和對細胞周期作用。我們又進一步選擇MG-63細胞作為研究對象,應用western blot、定量PCR、流式細胞術、小干擾RNA等實驗手段進一步探究人參皂苷Rg3誘導細胞自噬、凋亡以及細胞周期阻滯的具體作用機制。實驗結果:MTT實驗發(fā)現(xiàn)人參皂苷Rg3以劑量依賴的方式抑制三種骨肉瘤細胞...
【文章來源】：吉林大學吉林省 211工程院校 985工程院校 教育部直屬院校
【文章頁數(shù)】：110 頁
【學位級別】：博士
【部分圖文】：
人參皂苷Rg3的分子結構
28胞中，人參皂苷在160-1280nM的濃度范圍內，可以顯著抑制MG-63細胞的生長，1280nM時抑制作用最強。三種細胞系在24、48和72h時的抑制作用相近，如圖2.1-3所示。圖2.1MTT法檢測人參皂苷Rg3對MG-63細胞增殖的抑制作用Fig2.1MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationosteosarcomaMG-63cells
29圖2.2MTT法檢測人參皂苷Rg3對HOS細胞增殖的抑制作用Fig2.2MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaHOScells圖2.3MTT法檢測人參皂苷Rg3對U2OS細胞增殖的抑制作用Fig2.MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaU2OScells[
【參考文獻】：
期刊論文
[1]Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. Jian-Wen Jiang,Xin-Mei Chen,Xin-Hua Chen,Shu-Sen ZhengJian-Wen Jiang,Xin-Hua Chen,Shu-Sen Zheng,The Key Lab of Combined Multi-organ Transplantation,Ministry of Public Health,The Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China Xin-Mei Chen,The Department of Pharmacy,Shandong Traditional Chinese Medicine University,Jinan 250355,Shandong Province,China. World Journal of Gastroenterology. 2011(31)
[2]Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer[J]. XU Tian-min XIN Ying CUI Man-hua JIANG Xin GU Li-ping Department of Obstetrics and Gynaecology,Second Hospital of Jilin University,Changchun 130041,China Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China Department of Radiotherapy,Second Hospital of Jilin University,Changchun 130041,China. Chinese Medical Journal. 2007(07)
本文編號：3276336
【文章來源】：吉林大學吉林省 211工程院校 985工程院校 教育部直屬院校
【文章頁數(shù)】：110 頁
【學位級別】：博士
【部分圖文】：
人參皂苷Rg3的分子結構
28胞中，人參皂苷在160-1280nM的濃度范圍內，可以顯著抑制MG-63細胞的生長，1280nM時抑制作用最強。三種細胞系在24、48和72h時的抑制作用相近，如圖2.1-3所示。圖2.1MTT法檢測人參皂苷Rg3對MG-63細胞增殖的抑制作用Fig2.1MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationosteosarcomaMG-63cells
29圖2.2MTT法檢測人參皂苷Rg3對HOS細胞增殖的抑制作用Fig2.2MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaHOScells圖2.3MTT法檢測人參皂苷Rg3對U2OS細胞增殖的抑制作用Fig2.MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaU2OScells[
【參考文獻】：
期刊論文
[1]Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. Jian-Wen Jiang,Xin-Mei Chen,Xin-Hua Chen,Shu-Sen ZhengJian-Wen Jiang,Xin-Hua Chen,Shu-Sen Zheng,The Key Lab of Combined Multi-organ Transplantation,Ministry of Public Health,The Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China Xin-Mei Chen,The Department of Pharmacy,Shandong Traditional Chinese Medicine University,Jinan 250355,Shandong Province,China. World Journal of Gastroenterology. 2011(31)
[2]Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer[J]. XU Tian-min XIN Ying CUI Man-hua JIANG Xin GU Li-ping Department of Obstetrics and Gynaecology,Second Hospital of Jilin University,Changchun 130041,China Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China Department of Radiotherapy,Second Hospital of Jilin University,Changchun 130041,China. Chinese Medical Journal. 2007(07)
本文編號：3276336
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