研究背景:骨肉瘤是發(fā)源于間充質(zhì)干細(xì)胞的惡性腫瘤。骨肉瘤的發(fā)病年齡呈現(xiàn)雙峰分布,常發(fā)病于青少年或老年人。由于大多數(shù)的骨肉瘤患者在診斷時(shí)時(shí)就有轉(zhuǎn)移或微轉(zhuǎn)移性,故幾乎所有患者在手術(shù)切除的之前或之后都會(huì)接受多種藥物聯(lián)合化療�；颊咄褪芏容^差,且復(fù)發(fā)幾率較高。人參是一種原產(chǎn)于東北亞的草本植物,在東亞地區(qū)的傳統(tǒng)醫(yī)學(xué)中有著廣泛的應(yīng)用。作為人參中主要的活性物質(zhì)之一,人參皂苷Rg3的抗腫瘤作用在近年來越來越引起人們的重視。人參皂苷Rg3的甾體結(jié)構(gòu)使得它可以與細(xì)胞膜、膜結(jié)合離子通道、以及胞內(nèi)和胞外的受體相互作用來改變細(xì)胞的轉(zhuǎn)錄水平以來發(fā)揮抗腫瘤作用。研究方法:本實(shí)驗(yàn)應(yīng)用MTT實(shí)驗(yàn)、流式細(xì)胞術(shù)、細(xì)胞劃痕實(shí)驗(yàn)、Transwell細(xì)胞侵襲實(shí)驗(yàn)檢測了人參皂苷Rg3對骨肉瘤細(xì)胞系MG-63、HOS和U2OS增殖、凋亡、遷移、侵襲的影響和對細(xì)胞周期作用。我們又進(jìn)一步選擇MG-63細(xì)胞作為研究對象,應(yīng)用western blot、定量PCR、流式細(xì)胞術(shù)、小干擾RNA等實(shí)驗(yàn)手段進(jìn)一步探究人參皂苷Rg3誘導(dǎo)細(xì)胞自噬、凋亡以及細(xì)胞周期阻滯的具體作用機(jī)制。實(shí)驗(yàn)結(jié)果:MTT實(shí)驗(yàn)發(fā)現(xiàn)人參皂苷Rg3以劑量依賴的方式抑制三種骨肉瘤細(xì)胞... 

【文章來源】：吉林大學(xué)吉林省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：110 頁

【學(xué)位級別】：博士

【部分圖文】：

人參皂苷Rg3的分子結(jié)構(gòu)

28胞中，人參皂苷在160-1280nM的濃度范圍內(nèi)，可以顯著抑制MG-63細(xì)胞的生長，1280nM時(shí)抑制作用最強(qiáng)。三種細(xì)胞系在24、48和72h時(shí)的抑制作用相近，如圖2.1-3所示。圖2.1MTT法檢測人參皂苷Rg3對MG-63細(xì)胞增殖的抑制作用Fig2.1MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationosteosarcomaMG-63cells

29圖2.2MTT法檢測人參皂苷Rg3對HOS細(xì)胞增殖的抑制作用Fig2.2MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaHOScells圖2.3MTT法檢測人參皂苷Rg3對U2OS細(xì)胞增殖的抑制作用Fig2.MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaU2OScells[

【參考文獻(xiàn)】：
期刊論文
[1]Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. Jian-Wen Jiang,Xin-Mei Chen,Xin-Hua Chen,Shu-Sen ZhengJian-Wen Jiang,Xin-Hua Chen,Shu-Sen Zheng,The Key Lab of Combined Multi-organ Transplantation,Ministry of Public Health,The Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China Xin-Mei Chen,The Department of Pharmacy,Shandong Traditional Chinese Medicine University,Jinan 250355,Shandong Province,China.  World Journal of Gastroenterology. 2011(31)
[2]Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer[J]. XU Tian-min XIN Ying CUI Man-hua JIANG Xin GU Li-ping Department of Obstetrics and Gynaecology,Second Hospital of Jilin University,Changchun 130041,China Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China Department of Radiotherapy,Second Hospital of Jilin University,Changchun 130041,China.  Chinese Medical Journal. 2007(07)



本文編號(hào)：3276336