本文關(guān)鍵詞： 泡沫銅 葡萄糖 無酶葡萄糖傳感器 分級結(jié)構(gòu) 納米管　出處：《重慶師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：葡萄糖含量的分析檢測在臨床醫(yī)學(xué),食品監(jiān)測和生物科學(xué)等有著重要的作用。在臨床醫(yī)學(xué)上,葡萄糖含量的檢測主要還是依靠于基于葡萄糖氧化酶(GOx)的生物傳感器,但是酶是一種蛋白質(zhì),其易受外界環(huán)境(如溫度,pH)的影響而失活,因此基于電極表面直接電氧化的無酶葡萄糖傳感器成為現(xiàn)今的研究熱點(diǎn)。過渡金屬銅是構(gòu)建無酶葡萄糖傳感器的一種理想金屬,而多孔的泡沫銅由于其具有較大的比表面積顯示出了更大的研究潛能。隨著近年來納米技術(shù)的不斷發(fā)展,納米尺寸的銅及銅的化合物引起了廣泛研究,將納米材料生長在三維多孔泡沫銅基底上,多孔基底增大活性面積,促進(jìn)電極表面的電子傳遞,結(jié)合納米結(jié)構(gòu)材料的良好物理化學(xué)性質(zhì),進(jìn)一步提高了該類葡萄糖傳感器的靈敏度。本論文利用XRD、SEM、EDX等物理手段對三種材料進(jìn)行了微觀的形貌及結(jié)構(gòu)的分析,并采用循環(huán)伏安法(CV)和計時電流法(I-t)對材料的電化學(xué)性質(zhì)進(jìn)行表征,并將三種材料用于人體血清中葡萄糖含量的檢測。本文主要研究的內(nèi)容如下:(1)使用商業(yè)的泡沫銅,不需要任何預(yù)處理,直接用作工作電極進(jìn)行無酶葡萄糖傳感器的構(gòu)建,該傳感器的線性范圍為1μM-0.5 mM,靈敏度為5.85mA·mM~(-1)·cm~(-2),檢出限為0.5μM(S/N=3);(2)采用濕化學(xué)合成法,化學(xué)還原及電化學(xué)陽極處理的三步合成法制備了生長在泡沫銅上的銅-氫氧化銅納米分級結(jié)構(gòu),該納米材料在堿性條件下對葡萄糖氧化具有較高的催化活性,可用作電極材料構(gòu)建無酶葡萄糖傳感器的構(gòu)建,在電壓為0.5 V時,該傳感器具有寬的線性范圍為1μM~(-1) mM,高的靈敏度為9.18mA·mM~(-1)·cm~(-2)及低檢出限為0.45μM(S/N=3);(3)采用自犧牲模板法制備生長在泡沫銅上的CuS納米管,將該材料作為電極用于葡萄糖的電催化氧化,該材料表現(xiàn)出了良好的催化活性,在低的電壓0.45 V時,在0.1 M NaOH溶液中,具有兩個寬的線性范圍,在1μM-0.61 mM間,靈敏度為8.66 mA·mM~(-1)·cm~(-2),在0.61 mM~(-2).41 mM間,靈敏度為2.42 mA·mM~(-1)·cm~(-2),且具有一個低的檢出限為1μM(S/N=3)。
[Abstract]:Detection and analysis of the content of glucose in clinical medicine, plays an important role in food monitoring and biological science. In clinical medicine, mainly rely on the detection of glucose content based on glucose oxidase (GOx) biosensor, but the enzyme is a protein, its susceptibility to outside environment (such as temperature, pH) effect therefore, the inactivation of enzyme free glucose sensor has become a research hotspot of direct oxidation of the electrode surface. Based on the power transition metal copper is to build an ideal metal Enzymeless glucose sensor, and the porous copper foam because of its larger surface area showed greater potential for research. With the continuous development in recent years. Nanotechnology, copper and copper compounds of nanometer size has aroused extensive research, the growth of nano material in porous copper foam substrate, a porous substrate increases the active area of the electrode surface, and promote the The electron transfer, with good physical and chemical properties of nanostructured materials, to further improve the sensitivity of the glucose sensor. This paper uses XRD, SEM, EDX and other physical means to analyze the morphology and structure of three kinds of micro materials, using cyclic voltammetry (CV) and chronoamperometry (I-t) were used to characterize the electrochemical properties of materials, and three kinds of materials for the detection of glucose concentration in human serum. The contents of this paper are as follows: (1) the use of commercial copper foam, without any pretreatment, directly used as the working electrode to construct non enzymatic glucose sensor, the linear range of the sensor for 1 M-0.5 mM, the sensitivity is 5.85mA (-1) mM~ - cm~ (-2), the detection limit was 0.5 M (S/N=3); (2) using wet chemical synthesis, chemical reduction and electrochemical anodic treatment of three step synthesis of growth in copper foam 鐨勯摐-姘㈡哀鍖栭摐綰崇背鍒嗙駭緇撴瀯,璇ョ撼綾蟲潗鏂欏湪紕辨，

本文編號：1546475