本文關(guān)鍵詞： 熒光傳感 羅丹明B 頻移 譜帶重心 強(qiáng)度比　出處：《南昌航空大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：羅丹明B(RhB)染料屬于典型的有機(jī)發(fā)光材料,具有摩爾吸光系數(shù)高、光穩(wěn)定性好、波長(zhǎng)范圍寬以及量子產(chǎn)率高等優(yōu)點(diǎn),其熒光在可見(jiàn)光波段便于探測(cè),同時(shí)具有著很高的發(fā)光效率和優(yōu)異的生物相容性,基于RhB熒光的傳感方法及器件可以滿足在不同的生理、自然環(huán)境特定的測(cè)試需求。本文對(duì)羅丹明B未作任何改性處理,對(duì)由RhB或RhB/H610膠粘劑復(fù)合材料制作的熒光探頭的熒光光譜受溫度、應(yīng)力以及pH值等物理或化學(xué)量的影響進(jìn)行了多功能傳感特性研究、傳感器的設(shè)計(jì)研發(fā)。本文對(duì)傳統(tǒng)的熒光傳感方法進(jìn)行了歸納比較,對(duì)環(huán)境參數(shù)影響熒光的實(shí)驗(yàn)數(shù)據(jù)的分析主要采用譜峰頻移及新型表征方法“譜帶重心頻移法”、“自參考雙波長(zhǎng)強(qiáng)度比法”和“積分強(qiáng)度比法”等方法。實(shí)驗(yàn)中采用中心波長(zhǎng)405nm的藍(lán)紫色激光器作為激勵(lì)光源,通過(guò)設(shè)計(jì)不同的熒光傳感探頭和搭建相應(yīng)的熒光傳感系統(tǒng),分別對(duì)羅丹明B受溫度、應(yīng)力以及pH值影響的熒光特征譜進(jìn)行了全面的研究,實(shí)驗(yàn)總結(jié)出了羅丹明B熒光譜帶重心λB、自參考雙波長(zhǎng)強(qiáng)度比R、積分強(qiáng)度比RI等熒光特征信號(hào)傳感溫度T(單位℃)、壓應(yīng)力σ(單位MPa)以及pH值的多個(gè)不同的傳感方程,對(duì)熒光光纖傳感器重要的靜態(tài)指標(biāo)(重復(fù)性、遲滯特性等)進(jìn)行了分析,最后對(duì)工程化應(yīng)用作了初步嘗試。其中,主要實(shí)驗(yàn)結(jié)論是:(1)熒光傳感溫度:得到溫度傳感方程λB=589.2-0.049T,以及R=0.114+0.00204T(R=I(530nm)/I(580nm))。重心法傳感系統(tǒng)的重復(fù)性為0.8%FS,自參考雙波長(zhǎng)強(qiáng)度比法傳感系統(tǒng)的重復(fù)性為1.6%FS,二者均不存在明顯的遲滯現(xiàn)象。(2)熒光傳感應(yīng)力:得到應(yīng)力傳感方程λB=620.89-1.08σ+0.1σ2,以及R=0.82-0.01σ(R=I(625nm)/I(615nm))。重心法傳感系統(tǒng)的重復(fù)性為2.8%FS,自參考雙波長(zhǎng)強(qiáng)度比法傳感系統(tǒng)的重復(fù)性為2.1%FS。在RhB/H610膠粘劑復(fù)合材料譜帶重心波長(zhǎng)的遲滯特性實(shí)驗(yàn)中觀察到最大遲滯,約為11.8%FS。(3)熒光傳感pH:得到pH傳感方程λB=836.36-75.71pH+5.85pH2以及R=1.86-0.15pH(R=I(595nm)/I(585nm)),RI=0.17+0.04pH。(4)結(jié)合研發(fā)的羅丹明B熒光溫度傳感系統(tǒng)和熒光pH值傳感系統(tǒng),分別設(shè)計(jì)了微波環(huán)境中的水溫監(jiān)測(cè)和甘氨酸濃度測(cè)定實(shí)驗(yàn),驗(yàn)證了系統(tǒng)的實(shí)用性。兩種新型的傳感方法(譜帶重心法和自參考雙波長(zhǎng)強(qiáng)度比法)能夠顯著提高熒光傳感的精度和穩(wěn)定性。實(shí)驗(yàn)設(shè)計(jì)的熒光多功能傳感系統(tǒng)適用于微波醫(yī)學(xué)和強(qiáng)電磁干擾下的傳感測(cè)量。
[Abstract]:Rhodamine Bu RhB) dye is a typical organic luminescent material with high molar absorptivity, good light stability, wide wavelength range and high quantum yield. At the same time, it has high luminescence efficiency and excellent biocompatibility. The sensing methods and devices based on RhB fluorescence can meet different physiological conditions. In this paper, Rhodamine B has not been modified, and the fluorescence spectrum of fluorescent probe made of RhB or RhB/H610 adhesive composite material is subjected to the temperature. The effects of physical or chemical quantities such as stress and pH value on the multifunctional sensing characteristics and the design and development of the sensor were studied. In this paper the traditional fluorescent sensing methods were summarized and compared. The spectrum peak frequency shift and a new characterization method, "spectral barycenter shift method", are mainly used to analyze the experimental data of environmental parameters affecting fluorescence. The self-reference dual-wavelength intensity ratio method and the integrated intensity ratio method are used in the experiment. A blue-purple laser with a central wavelength of 405nm is used as the exciting light source in the experiment. By designing different fluorescence sensors and setting up the corresponding fluorescence sensing system, the fluorescence characteristic spectra of Rhodamine B affected by temperature, stress and pH value were studied comprehensively. Experimental results show that the center of gravity 位 B of Rhodamine B fluorescence spectrum band, the self-reference dual-wavelength intensity ratio R, the integral intensity ratio RI and other fluorescence characteristic signal sensing temperature T (unit 鈩，

本文編號(hào)：1447857