發(fā)布時(shí)間：2022-01-11 03:44

　　在發(fā)展中國(guó)家,為了適應(yīng)肉類和乳制品需求量的增加,給家畜提供穩(wěn)定和營(yíng)養(yǎng)豐富的飼料變得十分重要。甜高粱（Sorghum bicolor）、王草（Pennisetum purpuphoides）和象草（Pennisetum purpureum）廣泛種植于熱帶和亞熱帶地區(qū),是滿足動(dòng)物生產(chǎn)需要的優(yōu)良牧草資源。但是,其水溶性碳水化合物含量低、水分含量和緩沖能高,因此很難調(diào)制出優(yōu)質(zhì)的青貯飼料。近年來(lái),接種青貯用乳酸菌添加劑是一種提高青貯飼料發(fā)酵品質(zhì)的有效方法。然而,由于商業(yè)乳酸菌添加劑在熱帶和亞熱帶地區(qū)的適應(yīng)性差,常常不能提高牧草的青貯發(fā)酵品質(zhì)。一般來(lái)說(shuō),熱帶牧草上附生的乳酸菌具有良好的適應(yīng)性。因此,分離和應(yīng)用這些乳酸菌來(lái)調(diào)制優(yōu)質(zhì)青貯飼料,具有重要意義。本研究旨在從王草中分離、篩選、生理生化評(píng)價(jià)和鑒定出優(yōu)良乳酸菌菌株,驗(yàn)證其和商業(yè)乳酸菌添加劑提高甜高梁,象草和王草發(fā)酵品質(zhì)和家兔生長(zhǎng)性能的效果。研究結(jié)果如下。1.王草中分離的乳酸菌特性和分子鑒定對(duì)從王草中分離出的76株乳酸菌進(jìn)行耐熱試驗(yàn),篩選出的5株乳酸菌進(jìn)行形態(tài)學(xué)、生理生化特性分析和16S rRNA鑒定。結(jié)果如下,5個(gè)菌株均能在pH 3.5、4.0、... 

【文章來(lái)源】：南京農(nóng)業(yè)大學(xué)江蘇省 211工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：108 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
LIST OF ABBREIATIONS
CHAPTER-1
    1 GENERAL INTRODUCTION
        1.1 Importance of ensiling
        1.2 Tropical grasses
            1.2.1 Sweet sorghum(Sorghum bicolor)
            1.2.2 Elephant grass(Pennisetum purpureum)
            1.2.3 King grass(Pennisetum purpureophoides)
        1.3 Influence factors for improving the fennentation quality of silage
            1.3.1 Chemical factors for grass influence the fermentation quality of silage
                1.3.1.1 Dry matter
                1.3.1.2 WSC
                1.3.1.3 Buffering capacity
            1.3.2 Microorganisms factors of grass influence fermentation quality of silage
                1.3.2.1 LAB activity effect on the fermentation quality of silage
                1.3.2.2 Enterobacteria activity effect on the fermentation quality of silage
                1.3.2.3 Clostridia activity effect on the fermeniation quality of silage
                1.3.2.5 Bacilli activity effects on the fermentation quality of silage
        1.4 Additives improving silage fermentation quality
            1.4.1 Fennentation stimu lants
                1.4.1.1 Lactic acid bacteria
                1.4.1.2 Fermentable sugars
                1.4.1.3 Enzymes
            1.4.2 Fermentation inhibitors
        1.5 Effect of the isolated LAB on the fermentation quality of silage
        1.6 Effects of LAB treated silage on the animal performance
        1.7 Experimental design
        1.8 Aim of the present study
CHAPTER-2 CHARACTERISTICS AND MOLECULAR IDENTIFICATION OFLAB ISOLATED FROM KING GRASS
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Isolation of LAB from king grass
        2.2.2 LAB strains
        2.2.3 Physiological and morphological tests
        2.2.4 Extraction of LAB genomic DNA protocol
        2.2.5 Analysis of LAB identification by 16S rRNA sequencing
        2.2.6 Statistical analysis
    2.3 RESULTS
    2.4 DISCUSSION
    2.5 SUMMARY
CHAPTER-3 EFFECT OF THE ISOLATED LAB ON THE FERMENTATIONQUALITY OF SWEET SORGHUM SILAGE
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 LAB strains
        3.2.2 Silage making
        3.2.3 Chemical and microbial analysis
        3.2.4 Statistical analysis
    3.3 RESULTS
    3.4 DISCUSSION
    3.5 SUMMARY
CHAPTER-4 EFFECT OF OF THE ISOLATED LAB ON THE FERMENTATIONQUALITY OF ELEPHANT GRASS
    4.1 INTRODUCTION
    4.2 MATERIAL AND METHODS
        4.2.1 Experimental site
        4.2.2 Preparation of the experimental silages
        4.2.3 Collection of samples for fermentative quality and chemical composition
        4.2.4 Microbial population examination
        4.2.5 Statistical analysis
    4.3 RESULTS
    4.4 DISCUSSION
    4.5 SUMMARY
CHAPTER-5 EFFECTS OF THE ISOLATED LAB ON THE FERMENTATIONQUAILTY OF KING GRASS
    5.1 INTRODUCTION
    5.2 MATERIALS AND METHODS
        5.2.1 Preparation of silage
        5.2.2 Chemical analysis
        5.2.3 Microbial population
        5.2.5 Statistical analysis
    5.3 RESULTS
    5.4 DISCUSSION
    5.5 SUMMARY
CHAPTER-6 EFFECT OF THE ISOLATED LAB TREATED KING GRASSSILAGE ON THE PERFORMANCE OF RABBIT
    6.1 INTRODUCTION
    6.2 MATERIALS AND METHODS
        6.2.1 Test feedstuff
        6.2.2 Preparation of king grass silage
        6.2.3 Experimental Diets
        6.2.4 Experimental design and husbandry of rabbits
        6.2.5 Growth traits
        6.2.6 Sample of feed and feces collection
        6.2.7 Blood collection
        6.2.8 Biochemical analysis of silage samples
        6.2.9 Digestibility and biochemical analysis of feed and feces
        6.2.10 Analyses of blood biochemistry
        6.2.11 Statistical analysis
    6.3 RESULTS
    6.4 DISCUSSION
    6.5 SUMMARY
References
CONCLUSION
Innovation
ACKNOWLEDGEMENTS
Research publications



本文編號(hào)：3582021