胃腸道寄生蟲給全世界的畜牧業(yè)造成重大經濟損失。其中,奧斯特線蟲是寄生于反芻動物皺胃中的一種紅棕色的線蟲,導致胃炎（奧斯特線蟲�。�,全世界范圍內廣泛流行,尤其是在溫帶氣候地區(qū)。寄生線蟲病是很容易被忽視并且對人類的健康造成了巨大的威脅。據(jù)估計,最常見的土源性寄生線蟲為鞭蟲（毛首鞭形線蟲）,鉤蟲（十二指腸鉤口線蟲和美洲鉤蟲）以及蛔蟲（蛔蟲）等線蟲廣泛流行于世界較貧窮地區(qū),感染人數(shù)達到十億人。線粒體是有重要生化功能的細胞器,是真核細胞產生能量的場所。線粒體（mt）基因組位于細胞器內且獨立于核基因組,但彼此之間具有密切的關系。本研究對小反芻動物寄生線蟲奧斯特線蟲的完整線粒體（mt）基因組進行了DNA序列分析,并在預測的線粒體氨基酸序列數(shù)據(jù)集的基礎上,對其與旋毛蟲超家族成員的系統(tǒng)發(fā)育關系進行了研究。奧斯特線蟲的整個線粒體基因組是圓形的,長度為14,151bp。它由總共36個基因組成,包括12個編碼蛋白質的基因（PCGs）,2個核糖體RNA（rRNA）基因,22個轉移RNA（tRNA）基因和2個非編碼區(qū)域,所有的基因都在同一方向轉錄。進化分析結果顯示,基于12個蛋白質編碼基因的預測氨基酸序列與捻轉血... 

【文章來源】：華中農業(yè)大學湖北省 211工程院校 教育部直屬院校

【文章頁數(shù)】：135 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
Chapter 1 Review of Literature
    1.1 Background
    1.2 Progressive goat industry in China
    1.3 Parasitology
        1.3.1 Ostertagia spp
        1.3.2 Trichuris spp
    1.4 Overview of mitochondria and mt genome sequencing
        1.4.1 Mitochondria
        1.4.2 Evolution of mitochondria
        1.4.3 Mitochondrial Gene Content of Animals
        1.4.4 Mitochondrial Genome Structure
    1.5 Molecular Biology
        1.5.1 Phylogenetic studies
    1.6 Next generation sequencing(NGS)technology and parasite genomics
    1.7 Parasite Genomes
        1.7.1 Mitochondrial genomes of parasites
        1.7.2 Workflow based on NGS for mitochondrial genome
    Objectives
Chapter 2 Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily
    1.Introduction
    2.Materials and Methods
        2.1 Collection of worms and extraction of DNA
        2.2 Amplification of ITS-2 of Ostertagia trifurcata
        2.3 Amplification of long fragments and sequencing
        2.4 Gene annotation and sequence analysis
        2.5 Phylogenetic analysis on basis of dataset of amino acid sequences
    3.Results and Discussion
        3.1 ITS-2 analysis
        3.2 Organization,content and mt genome annotation
        3.3 Phylogenetic analysis
    4.Implications and Significance
    5.Conclusion
Chapter 3 Characterization of the complete mitochondrial genome of a whipworm Trichuris skrjabini(Nematoda:Trichuridae)
    1.Introduction
    2.Materials and Methods
        2.1 Parasite collection and isolation of DNA
        2.2 Trichuris skrjabini ITS-2 Amplification
        2.3 Amplification of short and long fragments by PCR
        2.4 Gene annotation and sequence analysis
        2.5 Phylogenetic analysis
    3.Results
        3.1 ITS-2 analysis
        3.2 Amplification of short and long fragments
        3.3 mt Genome Features
        3.4 Annotation
        3.5 Phylogenetic analysis
    4.Discussion
    5.Conclusion
Chapter4 SUMMARY
References
Appendix
    ITS-2 sequence of Ostertagia trifurcata
    Whole mt genome sequence of Ostertagia trifurcata(Accession No.MK227249)
    Features of Osteragai trifurcata mt genome
    ITS-2 sequence of Trichuris skrjabini
    Partial sequences of Trichuris skrjabini mitochondrial genes
    Whole mt genome sequence of Trichuris skrjabini(Accession No.MK333462)
    Features of Trichuris skrjabini mt genome
Acknowledgements
CURRICULUM VITAE


【參考文獻】：
期刊論文
[1]Herbal traditional Chinese medicine and its evidence base in gastrointestinal disorders[J]. Rolf Teschke,Albrecht Wolff,Christian Frenzel,Axel Eickhoff,Johannes Schulze.  World Journal of Gastroenterology. 2015(15)



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