茶樹精油是桃金娘科（Myrtaceae）白千層屬（Melaleucal）植物互葉白千層的新鮮枝葉經(jīng)水蒸汽蒸餾而得無色至淡黃色的精油,目前被廣泛應用于醫(yī)療、日化等領域中,具有良好的廣譜殺菌抑菌保健作用。課題組前期分析了茶樹精油的主要成分,并明確了茶樹精油及其主要活性成分4-松油醇對儲糧害蟲具有良好的殺蟲活性,但其對桃蚜的殺蟲作用尚未有報道。本文結(jié)合前期工作基礎,研究了茶樹精油及其主要成分對桃蚜的生物活性,探討了茶樹精油對桃蚜體內(nèi)基因表達的影響,并基于計算機輔助藥物設計技術明確了 4-松油醇與作用靶點的結(jié)合模式,通過轉(zhuǎn)錄組分析初步研究了桃蚜應答4-松油醇熏蒸脅迫的相關基因表達模式,為進一步研究4-松油醇對蚜蟲的殺蟲分子機制提供了前期基礎。主要結(jié)果如下:1、茶樹精油對桃蚜的驅(qū)避和觸殺活性研究生物活性測定實驗結(jié)果表明,茶樹精油對桃蚜的驅(qū)避和觸殺作用較好,且具有時間和劑量效應;濃度為2g/L茶樹精油處理試蟲48 h后,其對桃蚜的驅(qū)避指數(shù)（deterrence index,DI）為0.75;濃度為4g/L茶樹精油處理試蟲72 h后,其對桃蚜的觸殺效果可達92.58%;茶樹精油處理24 h對桃蚜觸殺... 

【文章來源】：安徽農(nóng)業(yè)大學安徽省

【文章頁數(shù)】：83 頁

【學位級別】：博士

【文章目錄】：
ACKNOWLEDGEMENTS
ABSTRACT
摘要
1 Literature review
    1.1 Introduction of M. persicae
        1.1.1 Pest status
        1.1.2 Life cycle
    1.2 Control measure
        1.2.1 Chemical management
        1.2.2 Essential oils
    1.3 Melaleuca alternifolia oil
    1.4 Justification
    1.5 Objective
    1.6 Significance of study
2 Insecticidal and repellent activity of M. alternifolia essential oil against M. persicae
    2.1 Materials and Method
        2.1.1 Bio-safety
        2.1.2 Myzus persicae culture
        2.1.3 Deterrent bioassay
        2.1.4 Contact bioassay
    2.2 Results and Discussion
        2.2.1 Repellency effect
        2.2.2 Contact toxicity
        2.2.3 Lethal concentrations
3 Gene expression profiling of M. aternifolia essential oil against M. persicae
    3.1 Materials and Methods
        3.1.1 Preparation of essential oil from M. alternifolia
        3.1.2 Myzus persicae culture
        3.1.3 Contact toxicity/ lethal concentration
        3.1.4 Real-Time Quantitative PCR (qRT-PCR)
        3.1.5 Analysis
    3.2 Results and Discussion
        3.2.1 Gene expression of OSD gene
        3.2.2 Gene expression of TOL gene
        3.2.3 Gene expression of ANT gene
        3.2.4 Gene expression of HSP 60
        3.2.5 Gene expression of FPPS Ⅰ
4 Explore insecticidal potential of M.alternifolia essential oil against M.persicae
    4.1 Computation Methods
        4.1.1 Sequence analysis and modelling
        4.1.2 Ligand preparation and molecular docking
        4.1.3 Molecular dynamics simulations
        4.1.4 Free energy calculation
    4.2 Result and Discussion
        4.2.1 In-silico studies
        4.2.2 Structural Description of the OSD,FPPS Ⅰ,and HSP 60 3D Model
        4.2.3 Molecular docking and molecular dynamics simulation
        4.2.4 Molecular dynamics simulation,MMGB/BSA
5 Fumigant effects and transcriptomix analysis of terpinen-4-ol on M.persicae
    5.1 Materials and Method
        5.1.1 Myzus persicae culture
        5.1.2 Bioassay of M.persicae
        5.1.3 Insecticide exposure,RNA extraction,library preparation and sequencing
        5.1.4 Bioinformatics analysis of RNA-Seq and differential expression analysis
    5.2 Results and Discussion
        5.2.1 Fumigant bioassay
        5.2.2 Synergistic effects
        5.2.3 Transcriptome sequencing analysis of M.persicae treated with terpinen-4-ol
        5.2.4 Go and KEGG analysis of DEGs
6 Conclusions
7 Reference
作者簡介



本文編號：3372655