雞滑液囊支原體病是一種急慢性傳染病,其特點(diǎn)是造成雞、鴨、鵝與野生鳥類滲出性滑膜囊炎和腱鞘炎。根據(jù)中國相關(guān)法律法規(guī)規(guī)定對動物的疾病防治過程中不能隨意濫用抗生素。而中草藥是天然草藥制成的添加劑且它的應(yīng)用為人們提供了更高質(zhì)量的畜產(chǎn)品。鑒于雞滑液囊支原體的風(fēng)險(xiǎn)和中草藥應(yīng)用的重要性,本研究旨在探討不同中草藥對寧夏株雞滑液囊支原體的影響。為測定相關(guān)中草藥的抑菌效果,選取12種中藥提取物對MS（雞滑液囊支原體）進(jìn)行了藥敏試驗(yàn),根據(jù)結(jié)果選取其中五種（大黃、黃連、金銀花、黨參、甘草）并組合成中藥復(fù)方制劑。本實(shí)驗(yàn)采用微量稀釋法（最小抑菌濃度）用無菌蝸牛試管法和牛津杯法進(jìn)行驗(yàn)證。結(jié)果表明,采用微量稀釋法,MS對大黃和黃連的敏感性最高,抑菌范圍為15.89～16.98 mg/ml,其次是金銀花抑菌范圍為25.86 mg/ml,黨參和甘草抑菌范圍為27.69-33.48 mg/ml。采用牛津杯法時(shí),抑菌圈直徑為24 mm用S均值表示,14～24 mm的抑菌直徑用I均值表示,14 mm以下的抑菌直徑用不敏感或抗性的R表示,得到結(jié)果大黃、黃連、金銀花、黨參、甘草抑菌圈均為S。對選取的五種中藥大黃、黃連、金銀花、黨參... 

【文章來源】：寧夏大學(xué)寧夏回族自治區(qū) 211工程院校

【文章頁數(shù)】：164 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
Abstract
Main Abbreviation
CHAPTER Ⅰ Introduction
    1. REVEW OF LITRATURE
        1.1 Medicinal plants
        1.2 History and evolution of Class Mollicutes
        1.3 Evolution and phylogeny
        1.4 Biology of Class Mollicutes
            1.4.1 Lack of cell wall
            1.4.2. Small genome
            1.4.3 Scavenging metabolism
        1.5 Avian Mycoplasmosis
            1.5.1 Etiology
        1.6 Mycoplasma Synoviae
            1.6.1 Morphology
            1.6.2 Isolation and Culture
            1.6.3 Physiological and Biochemical Characteristics
            1.6.4 Pathogenic mechanism of Mycoplasma Synoviae
            1.6.5 Resistance
            1.6.6 Epidemiology
        1.7 Clinical symptoms, pathological changes and histological changes
            1.7.1     Pathological changes
            1.7.2 Histological changes
            1.7.3 Diagnosis
            1.7.4 Molecular Biological Detection
        1.8 Prevention and control
            1.8.1 Drug Treatment
            1.8.2 Immunization
CHAPTER Ⅱ Extraction and sensitivity test of Chinese herbs
    2.1.Materials and methods of Chinese herbs extraction
        2.1.1 Reflux extraction method
        2.1.2 Impregnation method
        2.1.3 Reflux heat extraction method
        2.1.4 Advantages and disadvantages of Soxhlet extraction method
    2.2 Drug sensitivity test
    2.3 Results
        2.3.1 Drug sensitivity test of MIC
        2.3.2 Oxford cup method sensitivity test results
    2.4 Discussion
CHAPTER Ⅲ High performance liquid chromatography of Chinese herbs
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Chinese rhubarb(大黃)
        3.2.2 Codonopsis pilosula(黨參)
        3.2.3 Honeysuckle(金銀花)
        3.2.4 Licorice(甘草)
        3.2.5 Coptis chinensis(黃連)
    3.3 Results
        3.3.1 Calibration results of common peaks in five herbs
    3.4 Discussion
CHAPTER Ⅳ Herbal extracts safety test in mice
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Drug preparation for safety test
        4.2.2 Acute toxicity test of mice by intragastric administration
        4.2.3     Acute toxicity test in mice by intraperitoneal injection(pre-experimental dosagecalculation)
        4.2.4 Acute toxicity test of mice
    4.3 Drug safety results
        4.3.1 Acute toxicity test mice by intragastric administration
        4.3.2 Acute Toxicity Test Of Intraperitoneal Injection Of Mice
        4.3.3 Results of acute toxicity test of intraperitoneal injection of mice
    4.4 Discussion
CHAPTER Ⅴ Establishment of pathological model of MS and exploration of infection pathway
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Main Instruments
        5.2.2 Main Reagents
        5.2.3 Strain
        5.2.4 Preparation of Medium
        5.2.5 Bacterial Solution
        5.2.6 Grouping Treatment of birds chicken
        5.2.7 Treatment of Disease Material Samples and Identification of Mycoplasma in ChickenSynovial Sap
        5.2.8 Detection of serum antibodies against Mycoplasma Synoviae
    5.3 Result and Analysis
        5.3.1 Clinical symptoms
        5.3.2 Pathological examination
        5.3.3 Colony growth of Mycoplasma Synoviae
        5.3.4 Body Temperature Trend Map
        5.3.5 Identification of Mycoplasma in Chicken Synovial Sap by Specific Pohmerase ChainReaction
        5.3.6 Serum Antibody Test
    5.4 Discussion
CHAPTER Ⅵ Chicken pathological model
    6.1 Materials and Methods
        6.1.1 PCR procedure
        6.1.2 Amplification and identification of ms vlha specific gene pcr
    6.2 Protocol of ELISA
        6.2.1 Materials
        6.2.2 Procedure
    6.3 Results
        6.3.1 Chicken model infection results
        6.3.2 Chicken model result after supplementation of herbal extracts
        6.3.3 ELISA results
    6.4 Discussion
CHAPTER Ⅶ Intestinal Histomorphology of chicken after supplementation of compoundherbal medicine extracts
    7.1 Introduction
    7.2 Materials and Methods
    7.3 Results
    7.4 Duodenum
        7.4.1 Villus height(μm)
        7.4.2 Crypt depth(μm)
        7.4.3 Villus surface area(mm~2)
    7.5 Jejunum
        7.5.1 Villus height(μm)
        7.5.2 Crypt depth(μm)
        7.5.3 Villus surface area(mm~2)
    7.6 Ileum
        7.6.1 Villus height(μm)
        7.6.2 Crypt depth(μm)
        7.6.3 Villus surface area(mm~2)
    7.7 Discussion
CHAPTER Ⅷ Haematology and digestibility of chicken after supplementation ofcompound Chinese herbal medicine
    8.1 Methdology
    8.2 Hematology Material and Methods
        8.2.1 Packed cell volume(PCV)
        8.2.2 Hemoglobin (Hb) determination
        8.2.3 Total Leukocyte (WBC) count
        8.2.4 Red Cell (RBC) Count
    8.3 Digestibility trial materials and methods
        8.3.1 Proximate composition of feed and droppings
        8.3.2 Moisture and dry matter contents
        8.3.3 Crude protein
        8.3.4 Ether extract
        8.3.5 Crude fiber
    8.4 Results
        8.4.1 Result of hematology
        8.4.2 Result of digestibility trial
    8.5 Discussion
CHAPTER Ⅸ Effects of Compound Chinese Herbal extracts on Immune organs and Performance of chicken
    9.1 Introduction
    9.2 Materials and methods
    9.3 Results
    9.4 Discussion
Conclusion
References
ACKNOWLEDGEMENTS
DEDICATION



本文編號：3272578