高壓電燒傷對大鼠血清β-TG和PF-4的影響及烏斯他丁的干預(yù)作用
[Abstract]:Objective: In addition to the immediate heating injury of high-voltage electric burn, there is an unknown progressive injury. The study showed that the microcirculatory disturbance played a key role in the occurrence and development of progressive injury after electric burn, and found that the microcirculatory disturbance after electric burn was mainly caused by microvessel injury and micro-blood flow. The changes of platelet rheological behavior play an important role in the hemodynamic and rheology of the microcirculation. The platelet is one of the main components of the blood, which is an important factor that affects the hemodynamics and the rheology of the microcirculation. The high-voltage electric burn can stimulate the activation of the platelet, increase the aggregation of the platelet, enhance the adhesion and release the reaction, thereby increasing the microcirculation disturbance. But the molecular mechanism of the abnormal platelet-rheological behavior is not yet clear. In this study, the content of platelet factor 4 (PF-4) and the content of platelet factor 4 (PF-4) were measured in the early stage of high-voltage burn in rats, and the intervention of Ulinastatin (UTI) was used to further explore the related mechanisms and methods to prevent the abnormal changes of platelet rheological behavior after high-voltage burn. Methods: 240 healthy adult male SD rats were randomly divided into pseudo-high-voltage burn group (sham-wound group), high-voltage electric burn group (electric injury group), high-voltage burn composite saline treatment group (saline group), high-voltage burn composite UTI treatment group (UTI group), each group of 60 rats. In each group, the observation time was divided into 15 minutes before the injury, 5 minutes after the injury, 1 h, 2 h, 4 h and 8 h after the injury. Anesthetized rats (3ml/ kg) were injected intraperitoneally with 10% water-chloral solution, and after the anesthesia was successful, the rats were fixed on a special electric shock test bench, and the two 1cm-1cm electrode pieces were fixed to the left upper limb (current inlet) and the right lower limb (current outlet) of the rat respectively. Connect the test transformer and voltage regulator wire, turn on the power supply, adjust the voltage regulator to make the booster output voltage to 2k V, turn on the booster output power supply, and make the high-voltage current pass through the rat and the electric shock 3s, and the model is made to be successful. The false-wound group is not only connected with a wire to cause a false injury. In 5 minutes of electroinjury, 1% of UTI (2/ 104u/ kg, i.e. 2ml/ kg) was injected into the abdominal cavity of the UTI group, and 2ml/ kg of normal saline was injected intraperitoneally in the saline group. The solution was centrifuged at 3000 rpm for 10 min, and the supernatant was placed in the Eppendorf tube at-70.degree. C. for 10 min. The serum levels of triglyceride, PF-4 and the content of PF-4 in six groups of rats in each group were respectively detected by ELISA and double-sandwich antibody method. The results of statistical analysis were as follows: SPSS 2.0 statistical software and two-factor analysis of variance of the design were performed, and the multiple comparisons were tested by means of LSD. The level of the test was significantly higher than that of P 0.05. Results: The level of serum triglyceride-TG in the serum of the rats was higher than that of the sham-wound group (F = 172.186, P 0.01), and the serum level of TG-TG in the electroinjury group was affected by the time-dependent variable after electroinjury (F = 32.164, P0.01). At 4h and 8h, the pre-injury value (P-value was less than 0.01). The level of serum triglyceride-TG in the UTI group was lower than that of the saline group (F = 21.295, P0.01); the content of the serum triglyceride-TG in the UTI group was affected by the time-dependent variable after electroinjury (F = 109.115, P0.01). The mean level of PF-4 in the serum of the rats was higher than that of the sham-wound group (F = 29. 108, P 0.01), and the serum PF-4 content in the electroinjury group was affected by the time-dependent variable after electroinjury (F = 6.943, P0.01). The pre-injury value (P-value was less than 0.01) at 2h and 8h after electroinjury. The mean level of PF-4 in the UTI group was lower than that of the saline group (F = 37. 874, P0.01); the content of PF-4 in the UTI group was affected by the time-dependent variable after electroinjury (F = 15.026, P0.01), and was lower than that of the saline group at 1h, 2h, 4h, and 8h after electric injury (P <0.01). Conclusion: High-voltage burn can promote the release of platelet in rats, and raise the level of serum level-TG and PF-4 in the early stage of high-voltage burn in rats. UTI inhibited platelet-release in rats, and decreased the content of plasma-TG and PF-4 after high-voltage burn in rats, thus improving the rheological behavior of platelets.
【學(xué)位授予單位】:河北醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2017
【分類號】:R647
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