【摘要】：目的:建立和改進(jìn)大鼠深低溫停循環(huán)模型,初步評(píng)估四十分鐘DHCA對(duì)大鼠腦神經(jīng)功能的影響。方法:隨機(jī)選取20只SD大鼠,隨機(jī)分為實(shí)驗(yàn)組(n=10)和對(duì)照組(n=10),兩組大鼠均采用閉胸體外插管,實(shí)驗(yàn)組開(kāi)始體外循環(huán)后建立DHCA模型(對(duì)照組只插管不行體外循環(huán)),術(shù)后均采用Morris水迷宮和曠場(chǎng)實(shí)驗(yàn)研究大鼠腦神經(jīng)功能。結(jié)果:成功建立及改進(jìn)了四十分鐘DHCA大鼠模型,存活率達(dá)90%;經(jīng)過(guò)研究發(fā)現(xiàn):實(shí)驗(yàn)組大鼠Morris水迷宮的空間學(xué)習(xí)潛伏期更長(zhǎng),而且在目標(biāo)象限內(nèi)所呆的時(shí)間更短;曠場(chǎng)實(shí)驗(yàn)時(shí)在中央?yún)^(qū)域呆的時(shí)間更多,但移動(dòng)距離與對(duì)照組相差不大。結(jié)論:四十分鐘DHCA對(duì)大鼠腦神經(jīng)功能有損傷;四十分鐘DHCA大鼠模型是一個(gè)研究臨床DHCA腦損傷及腦保護(hù)可靠的實(shí)驗(yàn)平臺(tái)。
[Abstract]:Aim: to establish and improve the deep hypothermia circulatory arrest model in rats and to evaluate the effect of DHCA on the brain function of rats. Methods: twenty SD rats were randomly divided into experimental group (n = 10) and control group (n = 10). The experimental group established DHCA model after cardiopulmonary bypass (the control group was only intubated without cardiopulmonary bypass). The Morris water maze and open field experiment were used to study the brain nerve function of the rats. Results: the DHCA rat model was successfully established and improved for 40 minutes, and the survival rate was 90. The results showed that the Morris water labyrinth in the experimental group had a longer spatial learning latency and a shorter time in the target quadrant. In the open field experiment, the time spent in the central area was more, but the distance of movement was not different from that of the control group. Conclusion: forty minute DHCA can injure the brain function of rats, and 40 minutes DHCA rat model is a reliable experimental platform for the study of clinical DHCA brain injury and brain protection.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R654

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