多囊卵巢綜合征患者顆粒細胞中microRNA-200家族的表達及臨床意義
發(fā)布時間：2018-08-03 13:46
【摘要】：背景:非編碼微小RNAs(microRNAs,miRNAs)是非編碼單鏈小RNA,主要在轉(zhuǎn)錄后水平上調(diào)控基因表達,異常miR-200家族(包括:miR-200a、b、c、miR-429及miR-141五位成員)表達與胰島素抵抗和糖尿病等發(fā)病密切相關。多囊卵巢綜合征(polycystic ovary syndrome,PCOS)是生育期婦女最常見的內(nèi)分泌疾病,它的主要特征是:卵巢多囊樣改變,稀發(fā)或無排卵,高雄激素和胰島素抵抗。而目前認為胰島素抵抗和代償性高胰島素血癥在PCOS的發(fā)病中起著重要的作用。目的:探討PCOS患者顆粒細胞中miR-200家族的表達,分析其與胰島素抵抗的關系及臨床意義,為PCOS的發(fā)病機制及未來的診斷治療方向提供理論依據(jù)。方法:收集2015年10月至2016年6月期間,于山東大學附屬生殖醫(yī)院就診患者,年齡35歲,控制性促排卵長方案,行體外受精一胚胎移植(invitro fertilization and embryo transfer,IVF-ET)或卵胞i}內(nèi)單精子注射(intracytoplasmic sperm injection,ICSI)助孕;PCOS患者共收集120例,計算胰島素抵抗指數(shù)(homeostasis model assessment-insulin resistance,HOMA-IR),將 HOMA-IR ≥2.57患者,設為PCOS胰島素抵抗組(P-IR組),共78例;而HOMA-IR2.57的患者為PCOS非胰島素抵抗組(P-NIR組),共42例;同時收集因男方因素或輸卵管因素助孕,且卵巢功能正常患者72例,作為對照組。采用定量即時聚合酶鏈鎖反應(quantitative real time polymerase chain reaction,qRT-PCR)技術,檢測三組患者顆粒細胞中miR-200家族成員的表達情況,分析miR-200家族表達和三組患者臨床資料及其相互關系。結果:(1)臨床資料分析:P-IR組中體重指數(shù)、空腹血糖、空腹血胰島素、HOMA-IR、HOMA-β、收縮壓及收縮壓、甘油三酯、低密度脂蛋白,高于P-NIR組和對照組患者(P均0.05),而高密度脂蛋白低于后兩者(P0.05);超重或肥胖、高血糖、高血壓、脂代謝紊亂及代謝綜合征的發(fā)病率都高于P-NIR組和對照組患者(P均0.05);且促排卵用藥時間比P-NIR組和正常對照患者(Control組)長(P=0.047,P=0.011),促排卵用藥劑量比PCOS非胰島素抵抗患者(P-NIR組)大(P=0.001)。(2)miR-200家族在患者卵巢顆粒細胞中表達情況:miR-200b在P-IR組患者表達量明顯高于P-NIR組和Control組(P均0.05);而P-NIR組和Control組中,miR-200b表達無差異(P均≥0.05)。miR-141在P-IR和P-NIR兩組患者中表達量均明顯高于Control組患者(P均0.05)。(3)miR-200家族相對表達量與臨床指標的分析:在P-IR組中,miR-200b表達量與胰島素抵抗指數(shù)(HOMA-IR)呈正相關(r=0.532,P=0.002)。在P-NIR組中,miR-141與患者優(yōu)質(zhì)胚胎率正相關(r=0.576,P=O.001)。ROC曲線提示miR-141和miR-200c及二者聯(lián)合對PCOS的診斷有一定作用(AUC:0.736,0.632和0.717),而miR-141的診斷效能最優(yōu)。結論:PCOS胰島素抵抗患者高血壓等代謝方面遠期并發(fā)癥的患病率較高,對胰島素抵抗治療在應給予足夠重視。miR-200b高表達與PCOS胰島素抵抗正相關,可能是PCOS患者出現(xiàn)胰島素抵抗的重要病理機制之一;miR-141在PCOS胰島素抵抗和非抵抗患者中均異常高表達,提示miR-141可能還參與PCOS的發(fā)病,同時miR-141和PCOS非胰島素抵抗患者的優(yōu)質(zhì)胚胎率正相關,提示miR-141的表達可能影響胚胎發(fā)育,同時對PCOS有一定的診斷價值。
[Abstract]:Background: the non coding small RNAs (microRNAs, miRNAs) is a non coding single strand small RNA that mainly regulates gene expression at post transcriptional level. The expression of abnormal miR-200 family (including five members of miR-200a, B, C, miR-429 and miR-141) is closely related to the incidence of insulin resistance and diabetes. Polycystic ovary syndrome (polycystic ovary) is The most common endocrine disease of women in the childbearing period is characterized by polycystic changes of the ovary, dilute hair or anovulatory, hormone and insulin resistance in Kaohsiung. At present, insulin resistance and compensatory hyperinsulinemia play an important role in the pathogenesis of PCOS. Objective: To explore the expression of miR-200 family in granulosa cells of PCOS patients. The relationship and clinical significance of insulin resistance and its clinical significance were analyzed to provide a theoretical basis for the pathogenesis of PCOS and the future direction of diagnosis and treatment. Methods: from October 2015 to June 2016, the patients in the affiliated reproductive Hospital of Shandong University were aged 35 years old, control ovulation induction and embryo transfer (invitro ferti). Lization and embryo transfer, IVF-ET) or intracytoplasmic sperm injection (intracytoplasmic sperm injection, ICSI) were injected (intracytoplasmic sperm injection, ICSI); PCOS patients collected 120 cases, calculated insulin resistance index (homeostasis), and set up more than 2.57 patients, with 78 cases. OMA-IR2.57 patients were 42 cases of PCOS non insulin resistance group (group P-NIR), and 72 patients with normal ovarian function and normal ovarian function were collected at the same time as the control group. The quantitative real-time polymerase chain reaction (quantitative real time polymerase chain reaction, qRT-PCR) was used to detect the fine particle size in the three groups. The expression of miR-200 family members in the cell and the relationship between the miR-200 family expression and the clinical data of three groups of patients. Results: (1) clinical data analysis: body mass index (BMI), fasting blood glucose, fasting blood insulin, HOMA-IR, HOMA- beta, systolic and systolic pressure, triglyceride, low density lipoprotein in the P-IR group, higher than those in the P-NIR group and the control group ( P was 0.05), and high density lipoprotein was lower than that of the latter two (P0.05); the incidence of overweight or obesity, hyperglycemia, hypertension, lipid metabolism disorder and metabolic syndrome were higher than that of group P-NIR and control group (P 0.05), and the time of ovulation induction was longer than that of group P-NIR and normal control (Control group) (P=0.047, P=0.011), and the dosage of ovulation induction was more than PCOS. In patients with non insulin resistance (group P-NIR) (group P-NIR), (P=0.001). (2) the expression of the miR-200 family in the ovarian granulosa cells: the expression of miR-200b in the P-IR group was significantly higher than that in the P-NIR group and the Control group (P 0.05), while in the P-NIR group and the Control group, the miR-200b expression was no difference (P is equal to 0.05). Significantly higher than the Control group (P 0.05). (3) the analysis of the relative expression and clinical indicators of the miR-200 family: in the P-IR group, the miR-200b expression was positively correlated with the insulin resistance index (HOMA-IR) (r=0.532, P=0.002). In the P-NIR group, miR-141 was positively correlated with the high quality embryo rate (r=0.576, P=O.001). The combination of the two has a certain role in the diagnosis of PCOS (AUC:0.736,0.632 and 0.717), and the diagnostic efficiency of miR-141 is the best. Conclusion: the prevalence rate of long-term complications in PCOS insulin resistance patients is higher, and the high expression of.MiR-200b in insulin resistance therapy should be positively correlated with PCOS insulin resistance, which may be P COS patients are one of the most important pathological mechanisms of insulin resistance; miR-141 is highly expressed in PCOS insulin resistance and non resistance patients, suggesting that miR-141 may also participate in the pathogenesis of PCOS, and the high quality embryo rate of miR-141 and PCOS non insulin resistance patients is positively correlated. It suggests that the expression of miR-141 may affect the development of embryos and at the same time to PC. OS has a certain diagnostic value.
【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R711.75
[Abstract]:Background: the non coding small RNAs (microRNAs, miRNAs) is a non coding single strand small RNA that mainly regulates gene expression at post transcriptional level. The expression of abnormal miR-200 family (including five members of miR-200a, B, C, miR-429 and miR-141) is closely related to the incidence of insulin resistance and diabetes. Polycystic ovary syndrome (polycystic ovary) is The most common endocrine disease of women in the childbearing period is characterized by polycystic changes of the ovary, dilute hair or anovulatory, hormone and insulin resistance in Kaohsiung. At present, insulin resistance and compensatory hyperinsulinemia play an important role in the pathogenesis of PCOS. Objective: To explore the expression of miR-200 family in granulosa cells of PCOS patients. The relationship and clinical significance of insulin resistance and its clinical significance were analyzed to provide a theoretical basis for the pathogenesis of PCOS and the future direction of diagnosis and treatment. Methods: from October 2015 to June 2016, the patients in the affiliated reproductive Hospital of Shandong University were aged 35 years old, control ovulation induction and embryo transfer (invitro ferti). Lization and embryo transfer, IVF-ET) or intracytoplasmic sperm injection (intracytoplasmic sperm injection, ICSI) were injected (intracytoplasmic sperm injection, ICSI); PCOS patients collected 120 cases, calculated insulin resistance index (homeostasis), and set up more than 2.57 patients, with 78 cases. OMA-IR2.57 patients were 42 cases of PCOS non insulin resistance group (group P-NIR), and 72 patients with normal ovarian function and normal ovarian function were collected at the same time as the control group. The quantitative real-time polymerase chain reaction (quantitative real time polymerase chain reaction, qRT-PCR) was used to detect the fine particle size in the three groups. The expression of miR-200 family members in the cell and the relationship between the miR-200 family expression and the clinical data of three groups of patients. Results: (1) clinical data analysis: body mass index (BMI), fasting blood glucose, fasting blood insulin, HOMA-IR, HOMA- beta, systolic and systolic pressure, triglyceride, low density lipoprotein in the P-IR group, higher than those in the P-NIR group and the control group ( P was 0.05), and high density lipoprotein was lower than that of the latter two (P0.05); the incidence of overweight or obesity, hyperglycemia, hypertension, lipid metabolism disorder and metabolic syndrome were higher than that of group P-NIR and control group (P 0.05), and the time of ovulation induction was longer than that of group P-NIR and normal control (Control group) (P=0.047, P=0.011), and the dosage of ovulation induction was more than PCOS. In patients with non insulin resistance (group P-NIR) (group P-NIR), (P=0.001). (2) the expression of the miR-200 family in the ovarian granulosa cells: the expression of miR-200b in the P-IR group was significantly higher than that in the P-NIR group and the Control group (P 0.05), while in the P-NIR group and the Control group, the miR-200b expression was no difference (P is equal to 0.05). Significantly higher than the Control group (P 0.05). (3) the analysis of the relative expression and clinical indicators of the miR-200 family: in the P-IR group, the miR-200b expression was positively correlated with the insulin resistance index (HOMA-IR) (r=0.532, P=0.002). In the P-NIR group, miR-141 was positively correlated with the high quality embryo rate (r=0.576, P=O.001). The combination of the two has a certain role in the diagnosis of PCOS (AUC:0.736,0.632 and 0.717), and the diagnostic efficiency of miR-141 is the best. Conclusion: the prevalence rate of long-term complications in PCOS insulin resistance patients is higher, and the high expression of.MiR-200b in insulin resistance therapy should be positively correlated with PCOS insulin resistance, which may be P COS patients are one of the most important pathological mechanisms of insulin resistance; miR-141 is highly expressed in PCOS insulin resistance and non resistance patients, suggesting that miR-141 may also participate in the pathogenesis of PCOS, and the high quality embryo rate of miR-141 and PCOS non insulin resistance patients is positively correlated. It suggests that the expression of miR-141 may affect the development of embryos and at the same time to PC. OS has a certain diagnostic value.
【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R711.75
【相似文獻】
相關期刊論文 前10條
1 付曉強,徐雷鳴;喉顆粒細胞腫瘤一例[J];中華放射學雜志;2002年04期
2 周志瑜;和一個發(fā)育中的牙釉質(zhì)器相聯(lián)系的顆粒細胞團[J];國外醫(yī)學.口腔醫(yī)學分冊;1984年02期
3 鄭斐,呂時銘;白介素-6調(diào)節(jié)顆粒細胞雌孕激素分泌的作用[J];生殖與避孕;2001年02期
4 蘇小妹,張燕軍,劉鋒;大量前列腺巨嗜顆粒細胞1例[J];中國誤診學雜志;2003年09期
5 于寧昌;;人末梢單個核細胞產(chǎn)生顆粒細胞趨化因子[J];國外醫(yī)學(免疫學分冊);1981年03期
6 余英豪，鄭玉清，，張連郁;37例顆粒細胞雪旺氏瘤臨床病理分析[J];實用癌癥雜志;1994年01期
7 蘇小妹,張燕軍,劉鋒;尿檢中發(fā)現(xiàn)多量一過性前列腺巨嗜顆粒細胞1例[J];中國實驗診斷學;2003年06期
8 賈孟春;促性腺激素釋放激素高效類似物抑制人顆粒細胞分泌孕酮[J];國外醫(yī)學(計劃生育分冊);1983年01期
9 ;口腔及頜骨內(nèi)顆粒細胞病損的臨床病理、免疫組化及超微結構研究[J];國外醫(yī)學.口腔醫(yī)學分冊;1990年04期
10 王春梅,劉桂蓮,吳紅;消抗靈對離體培養(yǎng)的大鼠顆粒細胞雌孕激素生成的影響[J];牡丹江醫(yī)學院學報;1999年03期
相關會議論文 前10條
1 楊雪;周從容;;胰島素樣生長因子Ⅱ?qū)θ祟w粒細胞分泌功奶的影響[A];第二屆西部地區(qū)（12省區(qū)）婦產(chǎn)科學術會議論文匯編[C];2006年
2 沈健;馮云;龍雯晴;喇端端;;顆粒細胞水平抑制素B的表達及其在女性生殖中的意義[A];首屆滬浙婦產(chǎn)科學術論壇暨2006年浙江省婦產(chǎn)科學學術年會論文匯編[C];2006年
3 丁婷;羅愛月;楊書紅;賴志文;盧運萍;馬丁;王世宣;;小鼠卵巢顆粒細胞不同分離方法的比較[A];中華醫(yī)學會第三次全國絕經(jīng)學術會議暨絕經(jīng)相關問題學習班論文匯編[C];2011年
4 翁靜;諸定壽;;小鼠卵周圍顆粒細胞排卵后在輸卵管內(nèi)死亡過程的研究[A];第九次全國生殖生物學學術研討會論文摘要集[C];2003年
5 沈浣;;顆粒細胞與卵母細胞發(fā)育[A];中華醫(yī)學會第六次全國生殖醫(yī)學學術會議�？痆C];2012年
6 王春生;李晶;張滕子;寧方勇;樸善花;安鐵洙;;擬蜘蛛絲蛋白基因在小鼠顆粒細胞中的整合及其鑒定[A];中國畜牧獸醫(yī)學會動物解剖學及組織胚胎學分會第十六次學術研討會論文集[C];2010年
7 甄t熑
本文編號：2161930
本文鏈接：http://sikaile.net/shoufeilunwen/mpalunwen/2161930.html
最近更新
教材專著
