【摘要】：目的:檢測慢性阻塞性肺疾病患者與正常對照者痰液中MUC5AC、MUC5B及相關介質的含量,分析慢性阻塞性肺疾病急性加重組(肺脾氣虛證)、慢性阻塞性肺疾病穩(wěn)定期組(肺脾氣虛證)及正常對照組中MUC5AC、MUC5B及炎癥介質等指標數(shù)目的差異;揭示COPD急性加重型(肺脾氣虛證)呼吸道黏液的變化狀態(tài)及黏蛋白在慢性阻塞性肺疾病患者病情評估方面中的作用,說明黏蛋白的異常分泌和COPD急性加重型(肺脾氣虛證)密切相關,為臨床分型治療提供參考,為闡述“脾為生痰之源,肺為貯痰之器”的中醫(yī)理論提供科學的研究基礎。方法:對120名慢性阻塞性肺疾病的患者及60名正常人采用誘導痰的方法,分離痰標本,并進行細胞計數(shù)及分類;對分離的痰液上清液采用酶聯(lián)免疫吸附法(ELISA)檢測其中的MUC5AC、MUC5B。結果:慢性阻塞性肺疾病穩(wěn)定期、COPD急性加重期、正常對照組中,慢性阻塞性肺疾病患者中的MUC5AC、MUC5B增高,三組之間差異有統(tǒng)計學意義(P0.05),其中誘導痰在COPD急性加重期增加,慢性阻塞性肺疾病穩(wěn)定期減少,COPD急性加重期與正常對照組差異有統(tǒng)計學意義(P0.05),COPD急性加重期與COPD穩(wěn)定期差異有統(tǒng)計學意義(P0.05),COPD穩(wěn)定期與正常對照組差異有統(tǒng)計學意義(P0.05)。患者在誘導痰中,中性粒細胞、淋巴細胞及嗜酸性粒細胞均高于正常對照組,三組間差異有統(tǒng)計學意義(P0.05)。結論:MUC5AC、MUC5B與炎癥介質共同參與慢性阻塞性肺疾病的發(fā)病,黏蛋白的異常分泌與慢性阻塞性肺病關系密切,是慢性阻塞性肺病發(fā)病的物質基礎,對黏蛋白的深入研究可為闡述“脾為生痰之源,肺為貯痰之器”提供科學基礎。
[Abstract]:Objective: to detect the content of MUC5 ACU MUC5B and its related mediators in sputum of patients with chronic obstructive pulmonary disease (COPD) and normal controls. To analyze the difference of MUC5AC MUC5B and inflammatory mediators in acute exacerbation of chronic obstructive pulmonary disease (COPD), stable stage of COPD (deficiency of lung and spleen qi) and normal control group. To reveal the changes of respiratory mucus in acute exacerbation of COPD (deficiency of lung and spleen qi) and the role of mucin in the evaluation of chronic obstructive pulmonary disease (COPD). It is suggested that the abnormal secretion of mucin is closely related to the acute exacerbation of COPD (deficiency of lung and spleen qi), which provides a reference for clinical classification and treatment, and provides a scientific research basis for expounding the TCM theory that "spleen is the source of sputum and the lung is the organ for storing phlegm". Methods: the sputum samples were isolated from 120 patients with chronic obstructive pulmonary disease (COPD) and 60 normal controls, and the isolated sputum supernatants were detected by enzyme linked immunosorbent assay (Elisa). Results: the MUC5AC MUC5B in COPD patients was significantly higher than that in the control group (P0.05), and the induced sputum was increased in the acute exacerbation of COPD. Chronic obstructive pulmonary disease stable phase decreased COPD acute exacerbation phase and normal control group difference was statistically significant (P0.05) there was significant difference between COPD acute exacerbation stage and COPD stable phase (P0.05). There was significant difference between COPD stable phase and normal control group (P0.05). Patients in induced sputum, neutrophils, lymphocytes and eosinophil were higher than the normal control group, there was significant difference among the three groups (P0.05). Conclusion MUC5AC MUC5B and inflammatory mediators are involved in the pathogenesis of chronic obstructive pulmonary disease. The abnormal secretion of mucin is closely related to chronic obstructive pulmonary disease, and it is the material basis of the pathogenesis of chronic obstructive pulmonary disease. The further study of mucin can provide a scientific basis for explaining that the spleen is the source of sputum and the lung is the organ for storing phlegm.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R259

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相關期刊論文 前10條

1 李鴻霖;楊s，

本文編號：2136577