本文選題：中空生物玻璃 + 絲素蛋白��； 參考：《浙江理工大學(xué)》2017年碩士論文

【摘要】：近年來(lái),由于人口老齡化、交通事故頻發(fā)以及各種疑難疾病患者增多,造成骨組織和器官缺損的現(xiàn)象也越來(lái)越多。研制理想的用于骨組織修復(fù)的生物替代材料已經(jīng)成為骨組織工程的熱點(diǎn);而人體骨組織修復(fù)生物醫(yī)用紡織品亦成為現(xiàn)代生物醫(yī)用紡織品的重要研究方向之一。絲素蛋白是天然蠶絲脫膠后的高分子纖維蛋白,因其獨(dú)特的氨基酸,而具備良好的生物相容性和生物降解性,使得絲素蛋白在生物醫(yī)學(xué)領(lǐng)域尤其是骨修復(fù)材料方面越來(lái)越成為研究者關(guān)注的焦點(diǎn)。然而,絲素蛋白在人體中,與骨結(jié)合能力較差、生物活性不高,這些缺點(diǎn)限制了其在骨修復(fù)材料的應(yīng)用和發(fā)展。因此,本文通過(guò)絲素蛋白和中空生物玻璃球進(jìn)行有機(jī)/無(wú)機(jī)的復(fù)合,并通過(guò)加入聚環(huán)氧乙烷來(lái)提高絲素蛋白的彈性,制備新型的SF/PEO/HBG復(fù)合纖維膜,系統(tǒng)研究中空生物玻璃球和SF/PEO/HBG復(fù)合纖維膜的制備、理化性能以及生物活性。具體研究?jī)?nèi)容如下:(1)中空生物玻璃球的制備及性能研究采用改進(jìn)的溶膠-凝膠法,以聚丙烯酸(PAA)為軟模板來(lái)制備PAA@BG核-殼生物玻璃球,通過(guò)煅燒去除PAA后,得到中空生物玻璃球(HBG);并通過(guò)FE-SEM、TEM、FT-IR、EDS、TG等測(cè)試方法對(duì)制備的中空生物玻璃球的結(jié)構(gòu)、形貌及性能等進(jìn)行表征。通過(guò)考察制備時(shí)間對(duì)中空生物玻璃球的影響,得出72h為最佳反應(yīng)時(shí)間,鈣鹽、磷源能夠全部穩(wěn)定的包覆在中空SiO2表面,制備出形態(tài)及理化性能較好的中空生物玻璃球;熱力學(xué)分析表明,煅燒溫度為550℃,樣品中的有機(jī)成分PAA和未反應(yīng)完的鈣和磷已經(jīng)全部分解。(2)不同粒徑的中空生物玻璃球的調(diào)控及生物活性研究通過(guò)改變TEOS/PAA的質(zhì)量比,來(lái)調(diào)控中空生物玻璃球的粒徑,并通過(guò)FE-SEM、TEM、粒徑分布、FT-IR、EDS、XRD等測(cè)試方法對(duì)不同粒徑的中空生物玻璃球的結(jié)構(gòu)、形貌及性能等進(jìn)行研究;再通過(guò)體外生物活性測(cè)試,分析其誘導(dǎo)生成羥基磷灰石的能力以及演化,考察其生物活性。結(jié)果表明:中空生物玻璃球?yàn)樾螤钜?guī)整的球形、粒徑均一、單分散性良好,且具有明顯的中空結(jié)構(gòu),并且具有較強(qiáng)的誘導(dǎo)沉積羥基磷灰石的能力,生物活性良好。(3)SF/PEO/HBG骨修復(fù)復(fù)合材料的構(gòu)建及生物活性研究蠶絲經(jīng)脫膠、溶解成絲素蛋白溶液,加入PEO,攪拌均勻后制得SF/PEO混合溶液,再加入HBG,通過(guò)靜電紡絲制得SF/PEO/HBG復(fù)合纖維膜,并通過(guò)FE-SEM、FT-IR、EDS、XRD等測(cè)試方法對(duì)SF/PEO/HBG復(fù)合纖維膜的形貌及性能進(jìn)行表征;通過(guò)體外生物活性測(cè)試,分析其誘導(dǎo)生成羥基磷灰石的能力,考察其生物活性。結(jié)果表明:SF/PEO/HBG復(fù)合纖維膜經(jīng)乙醇處理后,纖維狀態(tài)良好,表面平整光滑,但纖維粗細(xì)不均勻,纖維間網(wǎng)絡(luò)結(jié)構(gòu)良好,并且具有強(qiáng)的羥基磷灰石形成能力,生物活性較好。由以上實(shí)驗(yàn)結(jié)果可知,SF/PEO/HBG復(fù)合纖維膜具有較好的理化性能和生物活性,有望成為一種用于骨缺損臨床治療的骨組織修復(fù)材料。
[Abstract]:In recent years, due to the aging of population, frequent traffic accidents and the increasing number of patients with various difficult diseases, the phenomenon of bone tissue and organ defects has become more and more. The development of ideal biomaterials for bone tissue repair has become a hot spot in bone tissue engineering, and biomedical textiles for human bone tissue repair have become one of the important research directions of modern biomedical textiles. Silk fibroin is a high molecular fibrin after degumming of natural silk. Because of its unique amino acid, silk fibroin has good biocompatibility and biodegradability. Silk fibroin has become the focus of researchers in biomedical field, especially in bone repair materials. However, silk fibroin has poor ability to bind to bone and low biological activity in human body. These shortcomings limit the application and development of silk fibroin in bone repair materials. Therefore, in this paper, a novel SF/ PEO / HBG composite fiber membrane was prepared by organic / inorganic composite of silk fibroin and hollow biological glass ball, and by adding polyethylene oxide to improve the elasticity of silk fibroin. The preparation, physical and chemical properties and biological activity of hollow bioglass ball and SFR / PEO / HBG composite fiber membrane were systematically studied. The main contents are as follows: (1) the preparation and properties of hollow biological glass spheres were studied. The PAABG core-shell biological glass spheres were prepared by modified sol-gel method and polyacrylic acid (PAA) as soft template. The PAA was removed by calcination. The hollow biological glass spheres (HBG) were prepared and the structure, morphology and properties of the hollow bioglass spheres were characterized by FE-SEMMA-TEMT-IRT-EDSTG and other methods. By investigating the effect of preparation time on hollow biological glass ball, the optimum reaction time is 72 h, calcium salt and phosphorus source can be coated on the surface of hollow Sio 2 stably, and the hollow biological glass ball with good morphology and physical and chemical properties can be prepared. The thermodynamic analysis shows that the organic components PAA and calcium and phosphorus in the sample have been completely decomposed at 550 鈩，

本文編號(hào)：2101044