本文選題：牽張成骨 + 胚胎成骨��； 參考：《廣西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:建立犬下頜骨牽張成骨、下頜骨骨折模型及幼犬下頜骨及胚胎下頜骨模型,通過(guò)高通量測(cè)序找到牽張成骨期間可能激活的與新骨快速形成相關(guān)的因子及信號(hào)通路。方法:實(shí)驗(yàn)共分為7組,其中實(shí)驗(yàn)組分別為牽張固定0周組(DO1)、牽張固定2周組(DO2)、犬30天胚胎組(E)、幼犬2周組(P)、骨折固定2周(對(duì)應(yīng)牽張組間歇期1周及牽張期1周)組(MF1)、骨折固定4周(對(duì)應(yīng)牽張組間歇期1周、牽張期1周及固定期2周)組(MF2)及對(duì)照組(AC)。每小組實(shí)驗(yàn)動(dòng)物為3只。DO1和DO2組中分別隨即選取3只健康成年中華田園犬,體重約15公斤,于其右側(cè)下頜骨施行下頜骨牽張成骨術(shù)。間歇期1周后開(kāi)始以1mm/天速度將右下頜骨向近中方向牽張,牽張期1周共預(yù)計(jì)牽張7mm后牽張結(jié)束,處死DO1組實(shí)驗(yàn)犬并收集右下頜骨牽張區(qū)標(biāo)本。DO2組完成牽張后固定2周,固定期結(jié)束后拍攝錐形束CT觀察成骨情況,然后處死動(dòng)物并收集右側(cè)下頜骨牽張區(qū)新生標(biāo)本,記錄標(biāo)本大體觀。MF1和MF2組分別隨即選取3只健康成年中華田園犬犬,體重約15公斤,于其右側(cè)下頜骨施行下頜骨骨折手術(shù),術(shù)式參考下頜骨牽張成骨,骨折間隙為7mm。分別于2周及4周后拍攝CBCT及處死取材,收集右下頜骨骨折間隙標(biāo)本。E組為孕30天的母犬,拍攝B超確認(rèn)天數(shù)后納入實(shí)驗(yàn)組。P組為隨機(jī)選取3只同種出生2周健康幼犬納入實(shí)驗(yàn)組。AC組隨機(jī)選取3只健康成年中華田園犬,體重約15公斤。處死E組、P組及AC組并收集各組右下頜骨標(biāo)本。將7組標(biāo)本進(jìn)行高通量LncRNA測(cè)序檢測(cè)。結(jié)果:1、DO1、DO2、MF1、MF2組動(dòng)物均順利完成手術(shù)及術(shù)后隨訪觀察。術(shù)后沒(méi)有實(shí)驗(yàn)犬出現(xiàn)相關(guān)嚴(yán)重并發(fā)癥,牽張器及鈦板固定牢固,未見(jiàn)松脫,亦未發(fā)現(xiàn)牽張器及鈦板的斷裂及變形。觀察所有實(shí)驗(yàn)犬行牽張前和牽張后右側(cè)尖牙牙位及骨折手術(shù)前后右側(cè)尖牙牙位,可發(fā)現(xiàn)牽張后及骨折后下頜尖牙相對(duì)于上頜尖牙有明顯的近中前移,說(shuō)明右側(cè)頜骨成功通過(guò)牽張及人為骨折延長(zhǎng)。2、從7組樣本中均提取出足量的總RNA,并分別成功構(gòu)建了用于lncRNA測(cè)序所需的cDNA文庫(kù)。成功完成了各組高通量lncRNA測(cè)序。3、本項(xiàng)目7組樣品數(shù)據(jù),總共有3504個(gè)lincRNA和3731個(gè)novel_lncRNA有表達(dá)。4、牽張組、胚胎組、幼犬組同正常對(duì)照組及骨折組相比差異表達(dá)的lncRNA中,TCONS_00170590有可能調(diào)控其靶基因Lix1參與牽張成骨的快速成骨過(guò)程。結(jié)論:1、Lnc RNA TCONS_00170590在DO1組、E組、P組中表達(dá)同AC組和MF1組相比有顯著差異,提示牽張成骨與胚胎及幼犬發(fā)育有共同點(diǎn)且不同于骨折愈合。2、LncRNA TCONS_00170590負(fù)調(diào)控其靶基因LIX1,并且可能參與調(diào)控牽張成骨過(guò)程中的血管新生和新骨快速形成。3、牽張成骨中除與胚胎及幼犬共同的生長(zhǎng)調(diào)節(jié)因素外,牽張力、微創(chuàng)傷等因素可能是其更快速成骨的原因。
[Abstract]:Objective: to establish canine mandibular distraction osteogenesis, mandibular fracture model and pup mandibular and embryonic mandibular model, and to find out the factors and signal pathways related to the rapid formation of new bone during distraction osteogenesis by high-throughput sequencing. Methods: the experiment was divided into 7 groups. The experimental groups were: distraction fixation 0 week group (do 1), distraction fixation group 2 weeks group (do 2), dog 30 days embryo group (E), puppies 2 weeks group (P), fracture fixation 2 weeks group (corresponding to distraction group 1 week and distraction period 1 week) group (MF1), fracture fixation 4 weeks (P). The intermission period of the distraction group was 1 week. The distraction period of 1 week and fixed period of 2 weeks) group (MF2) and control group (AC). Three healthy adult Chinese pastoral dogs, weighing about 15 kg, were selected from each group. The mandibular distraction osteogenesis was performed on the right mandible. One week after the intermission period, the right mandible was stretched to the proximal Chinese side at the rate of 1mm/ day, and the distraction was expected to end after the distraction of 7mm in one week. The dogs of group DO1 were killed and the specimens of the distraction area of right mandible were collected. The specimens of the distraction area of the right mandible were fixed for 2 weeks after distraction in group DO2. At the end of the fixation period, the osteogenesis was observed by tapered CT, then the animals were killed and the newborn specimens of the right mandibular distraction region were collected. Three healthy adult Chinese pastoral dogs were selected immediately in the gross observation. MF1 and MF2 groups, respectively. The mandibular fracture was performed on the right mandible of the mandible, weighing about 15 kg. The mandibular distraction osteogenesis was referred to the procedure, and the fracture space was 7 mm. CBCT was taken at 2 and 4 weeks later, and the specimens of right mandibular fracture space were collected. Group E was 30 days pregnant. Three healthy puppies of the same type were randomly selected from the experiment group and AC group, and 3 healthy adult Chinese pastoral dogs were randomly selected, weighing about 15 kg. Group E, group P and group AC were sacrificed and the right mandibular specimens were collected. High throughput LncRNA sequencing was performed in 7 groups of specimens. Results all the animals in the two groups were successfully operated and followed up. There were no serious complications in dogs after operation. The tensioner and titanium plate were firmly fixed without loosening, and the fracture and deformation of the distractor and titanium plate were not found. By observing the right canine tooth position before and after distraction and the right canine tooth position before and after fracture operation, we found that the mandibular canine teeth after distraction and fracture had a significant proximal anterior movement to the maxillary canine teeth. The results showed that the right jaw bone was successfully lengthened by distraction and artificial fracture. The total RNAs were extracted from 7 groups of samples and the cDNA libraries for lncRNA sequencing were constructed successfully. High throughput lncRNA sequencing. 3 was successfully completed in each group. In this project, there were 3504 lincRNAs and 3731 Novelln cRNAs expressed in 7 groups of sample data, including distraction group, embryo group, distraction group and embryo group. TCONS00170590 in the differentially expressed lncRNA in the puppies, compared with the normal control group and fracture group, may be involved in the rapid osteogenesis of distraction osteogenesis by regulating its target gene Lix1. Conclusion the expression of TCONS00170590 in TCONS00170590 is significantly different from that in AC and MF1 groups, and the expression of TCONS00170590 in P group is significantly higher than that in AC group and MF1 group. These results suggest that distraction osteogenesis has something in common with the development of embryos and puppies and is different from fracture healing. 2LncRNA TCONS00170590 negatively regulates its target gene LIX1, and may be involved in the regulation of angiogenesis and rapid formation of new bone during distraction osteogenesis. In addition to common growth regulation factors with embryos and puppies, Some factors, such as stretch and microtrauma, may be the causes of faster osteogenesis.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R782.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬駿;黃嵐;;長(zhǎng)鏈非編碼RNA在心血管系統(tǒng)中的研究進(jìn)展[J];中國(guó)循環(huán)雜志;2016年12期

2 宋娜娜;柴志欣;鐘金城;;長(zhǎng)鏈非編碼RAN的研究進(jìn)展[J];生物技術(shù)通報(bào);2016年09期

3 武成聰;陳佳濱;蔡偉良;寧寅寬;李強(qiáng);;骨形態(tài)發(fā)生蛋白誘導(dǎo)骨髓間充質(zhì)干細(xì)胞成骨分化的研究進(jìn)展[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2014年21期

4 Maya Fakhry;René Buchet;David Magne;Eva Hamade;Bassam Badran;;Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts[J];World Journal of Stem Cells;2013年04期

5 周諾;黃旋平;江獻(xiàn)芳;宋繼傳;李華;謝慶條;;骨形態(tài)發(fā)生蛋白-2基因修飾骨髓間充質(zhì)干細(xì)胞復(fù)合對(duì)氧化聚乙烯和聚丙烯共聚物移植促進(jìn)兔下頜骨牽張成骨的實(shí)驗(yàn)研究[J];華西口腔醫(yī)學(xué)雜志;2013年03期

6 黃旋平;周諾;楊媛媛;江獻(xiàn)芳;李華;謝慶條;;hBMP-2基因修飾自體BMSCs移植促進(jìn)兔下頜骨牽張成骨新骨形成的X線分析[J];實(shí)用口腔醫(yī)學(xué)雜志;2012年04期

7 黃旋平;周諾;江獻(xiàn)芳;宋繼傳;李華;謝慶條;;人骨形態(tài)發(fā)生蛋白2基因修飾自體骨髓間充質(zhì)干細(xì)胞移植促進(jìn)兔下頜骨牽張成骨實(shí)驗(yàn)的組織形態(tài)學(xué)觀察[J];中國(guó)組織工程研究;2012年23期

8 周諾;;牽張成骨研究中的幾個(gè)熱點(diǎn)問(wèn)題[J];口腔頜面外科雜志;2011年04期

9 婁新田;房兵;沈國(guó)芳;江凌勇;;牽張成骨區(qū)牙移動(dòng)動(dòng)物模型的建立[J];上�？谇会t(yī)學(xué);2011年01期

10 蔣金玲;于穎彥;;血管生成擬態(tài)是對(duì)經(jīng)典腫瘤血管生成理論的補(bǔ)充[J];內(nèi)科理論與實(shí)踐;2010年02期

相關(guān)碩士學(xué)位論文 前1條

1 戚婧倩;犬骨髓EPCs分離、培養(yǎng)、鑒定及其表面標(biāo)志物CD133在非血管化輸送盤(pán)牽張成骨中表達(dá)的研究[D];廣西醫(yī)科大學(xué);2015年

，

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