本文選題：白藜蘆醇 + 缺血/再灌注損傷　； 參考：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:選擇頸內(nèi)動(dòng)脈栓線法構(gòu)建大腦中動(dòng)脈阻塞(MCAO)腦缺血?jiǎng)游锬Ｐ?分析注射各劑量白藜蘆醇后對(duì)動(dòng)物模型腦保護(hù)呈現(xiàn)出的劑量效應(yīng)關(guān)系。方法:1.動(dòng)物分組:75只SD大鼠,雄性,清潔級(jí),體重均處于220~250g的范圍內(nèi)。隨機(jī)將研究動(dòng)物分成下列5組(15只/組):模型組、假手術(shù)組、低劑量(10 mg/kg)組、中劑量(30 mg/kg)組、高劑量(60mg/kg)組。構(gòu)建局造性腦缺血/再灌注動(dòng)物模型,觀察海馬神經(jīng)元的形態(tài)學(xué)特征再灌注處理后第7天,通過水合氯醛(10%)對(duì)大鼠施麻后,從心臟處灌注磷酸鹽緩沖液200ml(0.1M,4°C),再給予多聚甲醛200ml(4%),最終可觀察到動(dòng)物四肢僵硬,將大腦取出,置于固定液內(nèi)保存至4°C溫度下一整夜。取視交叉-大腦橫裂區(qū)域,行石蠟固定包埋后,行均勻冠狀切片,4μm/片,經(jīng)常規(guī)脫蠟后,完成HE染色,于100倍顯微鏡下了解海馬CA1區(qū)具體的神經(jīng)元形態(tài)特征,并計(jì)算海馬CA1區(qū)(1mm)受損錐體神經(jīng)元的水平。結(jié)果:相比于假手術(shù)組,缺血再灌注常引起模型組動(dòng)物具有更高的腦組織含水量;而相比于模型組,不同劑量組動(dòng)物具有更低的腦組織含水量。光鏡下觀察海馬神經(jīng)元形態(tài)學(xué)特點(diǎn)結(jié)果顯示:假手術(shù)組能夠表現(xiàn)出完整的錐體神經(jīng)元形態(tài);而模型組能夠觀察到諸多異常特征,如變性、炎癥及壞死等;注射中等劑量白藜蘆醇能夠在較大程度上緩解神經(jīng)元受損情況;白藜蘆醇、缺血均不會(huì)明顯的影響齒回神經(jīng)元的形態(tài)特征。結(jié)論:白藜蘆醇能夠有效的保護(hù)并改善局灶性缺血/再灌注受損情況,其中療效最佳的為注射中等劑量的白藜蘆醇組。
[Abstract]:Aim: to establish the animal model of middle cerebral artery occlusion (MCAO) with internal carotid artery occlusion and to analyze the dose-effect relationship of brain protection after different doses of resveratrol. Method 1: 1. The animals were divided into five groups: 75 SD rats, male, clean grade, weight in the range of 250 g. The study animals were randomly divided into the following five groups: model group, sham operation group, low dose 10 mg / kg group, middle dose 30 mg / kg group and high dose 60 mg / kg group. The morphological characteristics of hippocampal neurons were observed 7 days after reperfusion. The rats were anesthetized by chloral hydrate. Phosphate buffer (200ml) was perfused from the heart with a phosphate buffer (200ml) 0.1MU 4 擄C, and then was given paraformaldehyde (200ml). Finally, the stiff limbs of the animal were observed. The brain was removed and stored in the fixed solution for one night under 4 擄C temperature. The optic chiasma and the transverse fissure region of the brain were taken. After paraffin fixation, 4 渭 m / slice of uniform coronal section was performed. After dewaxing, HE staining was completed, and the specific neuronal morphological characteristics of hippocampal CA1 area were studied under 100 times microscope. The level of injured pyramidal neurons in hippocampal CA 1 area was calculated. Results: compared with sham operation group, ischemia reperfusion often caused higher water content of brain tissue in model group, and lower water content of brain tissue in different dose groups compared with model group. The morphological characteristics of hippocampal neurons were observed under light microscope: sham-operated group could show complete pyramidal neuron morphology, while model group could observe many abnormal features, such as degeneration, inflammation and necrosis. The moderate dose of resveratrol could alleviate the neuronal damage to a great extent, and neither resveratrol nor ischemia could significantly affect the morphological characteristics of dentate gyrus neurons. Conclusion: resveratrol can effectively protect and improve focal ischemia / reperfusion injury.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R743.3

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本文編號(hào)：2013229