本文選題：二苯乙烯苷 + 二蒽酮　； 參考：《北京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:傳統(tǒng)植物藥何首烏(Radix Polygoni Mulfiflori)為寥科(Polygonaceae),何首烏屬(Fallopia Adans)多年生草本植物何首烏Polygonum multiflorum Thunb.的塊根。本論文對(duì)近年來(lái)何首烏的化學(xué)成分和藥理活性的研究進(jìn)行了較為全面概括,發(fā)現(xiàn)近年來(lái)對(duì)何首烏抗糖尿病活性的有效成分并不明確,因此本課題對(duì)何首烏具有抗糖尿病活性的55%乙醇部位進(jìn)行了研究,以期能發(fā)現(xiàn)具有活性的單體化合物。方法:運(yùn)用現(xiàn)代各種色譜技術(shù)(大孔吸附樹(shù)脂、SephadexLH-20、反相硅膠柱色譜和液相制備)對(duì)何首烏55%乙醇部位進(jìn)行分離純化,運(yùn)用核磁共振等光譜技術(shù)對(duì)化合物進(jìn)行結(jié)構(gòu)鑒定,并對(duì)分離得到的化合物進(jìn)行了降血糖活性篩選。結(jié)果:從何首烏中共分離鑒定了 27個(gè)化合物:Thunberginol C 6-O-β-D-glucopyran-oside(1),2,3,5,4'-四羥基二苯乙烯-2-O-β-L-鼠李糖苷(2),對(duì)羥基苯甲醛(3),反式-N-咖啡酰酪胺(4),白藜蘆醇-3-O-(2"-O-沒(méi)食子�；�)-β-D-葡萄糖苷(5),2,3,5,4'-四羥基二苯乙烯-2-0-(3〃-沒(méi)食子�；�)-β-D-葡萄糖苷(6),決明酮-8-O-β-D-葡萄糖苷(7),6-hydroxymusizin-8-O-β-D-glucoside(8),2,3,5,4'-四羥基二苯乙烯-2-O-(2〃-對(duì)羥基苯甲酰基)-β-D-葡萄糖苷(9),2,3,5,4'-四羥基二苯乙烯-2-0-(2〃-沒(méi)食子�；�)-β-D-葡萄糖苷(10),反式-2,3,5,4'-四羥基二苯乙烯-2-O-β-D-葡萄糖苷(11),順式-2,3,5,4'-四羥基二苯乙烯-2-O-β-D-葡萄糖苷(12),苜蓿素-6-0-葡萄糖苷(13),大黃素-8-O-β-D-葡萄糖苷(14),穆坪馬兜鈴酰胺(15),N-反式阿魏�；�-3-甲基多巴胺(16),槲皮素(17),苜蓿素(18),何首烏丙素(19),兒茶素(20),表兒茶素(21),土大黃苷(22),cis-大黃素-大黃素二蒽酮(23),trans-大黃素-大黃素二蒽酮(24),白藜蘆醇(25),白藜蘆醇苷(26),決明酮-8-0-(6'-O-乙�；�)-β-D-葡萄糖苷(27)。結(jié)論:化合物為1、2為新化合物,化合物4、5、13、27為首次從何首烏中分離得到。對(duì)何首烏各粗提部位以及單體成分進(jìn)行降血糖活性篩選,結(jié)果表明,55%乙醇部位以及化合物6、9、10、23和24具有較好的降血糖活性。
[Abstract]:Objective: Radix Polygoni Mulfiflori (Radix Polygoni Mulfiflorii) is a perennial herb of Polygonaceaeae (Fallopia Adansa) and Polygonum multiflorum Thunb. The root of In this paper, the chemical constituents and pharmacological activities of Polygonum multiflorum in recent years were summarized, and it was found that the effective components of the anti-diabetic activity of Polygonum multiflorum were not clear in recent years. Therefore, 55% ethanol fraction with antidiabetic activity of Polygonum multiflorum was studied in order to find active monomers. Methods: all kinds of modern chromatographic techniques (Sephadex LH-20, reverse silica gel column chromatography and liquid phase preparation) were used to separate and purify the 55% ethanol fraction of Polygonum multiflorum. The hypoglycemic activity of the isolated compounds was screened. Results: a total of 27 compounds: Thunberginol C 6-O- 尾 -D-glucopyran-osideside 1: Thunberginol C 6-O- 尾 -D-glucopyran-oside were isolated and identified from Radix Polygonum multiflorum. Twenty-seven compounds were isolated and identified, including Thunberginol C 6-O- 尾 -D-glucopyran-oside, tetrahydroxy distyrene -2-O- 尾 -L-rhamnoside, p-hydroxybenzaldehyde, trans--N-caffeoyltyramine, resveratrol, 3-O-O-2 "-O- 尾 -D- glucoside 235H4. Distyrene-2-0-trimethylidene 3 "- 尾 -D- glucoside 6", cassimone -8-O- 尾 -D- glucoside 7 "-hydroxymusizin-8-O- 尾 -D-glucoside 8-D-glucoside 8" -2-O-2 "-4-hydroxybenzoylformyl-尾 -dihydroxystyrene-2-O-2" -p-hydroxybenzoylformyl-尾 -D- 尾 -glucoside 933- (4-) -tetrahydroxy distyrene-2-0-2 "- 尾 -Dglucoside-尾 -glucose Glucoside 10C, trans--2O3O5- tetrahydroxy stilbene -2-O- 尾 -Dglucoside, cis-2O3O3O3O- 尾 -tetrahydroxy stilbene -2-O- 尾 -Dglucoside 12C, alfalfa -6-0- glucoside 1313, emodin -8-O- 尾 -D- glucoside 14, Aristolocham 15N- reverse ferulyl -3methylpolypolymethylethylmandeloside (Aristolochia muppingensis 15N), Alfalfa (alfalfa) -6-0- glucoside 1313, emodin-8-O- 尾-glucoside 14 Barramine 16, quercetin 17, alfalfa 18, Polygonum multiflorum 19, catechin 20, epigallocatechin 21, chrysophanoside 2 22cis-emodin dianthronone 23, emodin dianthrone 24, resveratrol 25, resveratrol 26, cassiazone -8-0-6 -O- acetyl-尾 -D- glucoside 27. Conclusion: compound 1: 1 is a new compound, and compound 4: 5, 1, 13, 27 is isolated from Radix Polygoni multiflorum for the first time. The hypoglycemic activity of crude extracts and monomers of Radix Polygoni multiflorum was screened. The results showed that 55% ethanol fraction and the compounds 60.99 10, 23 and 24 had better hypoglycemic activity.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R284.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張靜嫻;崔艷梅;;中藥何首烏的化學(xué)成分研究[J];中國(guó)中藥雜志;2016年17期

2 梅雪;余劉勤;陳小云;周春陽(yáng);;何首烏化學(xué)成分和藥理作用的研究進(jìn)展[J];藥物評(píng)價(jià)研究;2016年01期

3 劉瑩;王菲;;何首烏提取物對(duì)脂肪酸合酶的抑制作用[J];生物技術(shù)世界;2015年09期

4 陳俊;李興;朱麗英;潘衛(wèi);;制何首烏對(duì)鏈脲佐菌素糖尿病大鼠降血糖作用及其機(jī)制探討[J];中國(guó)臨床藥理學(xué)雜志;2015年01期

5 李登科;李寶賽;崔寶弟;孫震曉;;何首烏中大黃素-8-O-β-D-葡萄糖苷的分離純化與體外抗癌活性研究[J];癌變·畸變·突變;2014年06期

6 趙慧男;陳麗麗;黃曉君;王英;李藥蘭;葉文才;;何首烏中一個(gè)新的色原酮糖苷[J];中國(guó)中藥雜志;2014年08期

7 樓招歡;呂圭源;俞靜靜;;何首烏成分、藥理及毒副作用相關(guān)的研究進(jìn)展[J];浙江中醫(yī)藥大學(xué)學(xué)報(bào);2014年04期

8 魏琦;岳永德;湯鋒;孫嘏;王淑英;喻謹(jǐn);;苦竹葉化學(xué)成分研究[J];天然產(chǎn)物研究與開(kāi)發(fā);2014年01期

9 潘小翠;邊才苗;管銘;;何首烏不同溶劑提取液的體外抑菌活性研究[J];湖北農(nóng)業(yè)科學(xué);2013年18期

10 張綿松;劉新;孟秀梅;賈愛(ài)榮;張永剛;夏雪奎;袁文鵬;劉昌衡;;生何首烏體外抗氧化活性及抗菌活性的研究[J];食品科技;2012年08期

相關(guān)會(huì)議論文 前1條

1 張?zhí)m;李林;李雅莉;樸景華;;二苯乙烯甙拮抗β-淀粉樣蛋白及過(guò)氧化氫致神經(jīng)細(xì)胞損傷[A];中國(guó)藥理學(xué)會(huì)第十屆全國(guó)神經(jīng)學(xué)術(shù)會(huì)議暨浙江省藥理學(xué)會(huì)2002年年會(huì)論文摘要集[C];2002年

，

本文編號(hào)：1998102