發(fā)布時(shí)間：2018-05-29 23:25

  本文選題：多糖 + 響應(yīng)面��； 參考：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：煙管菌屬多孔菌科真菌,有關(guān)煙管菌生物學(xué)效應(yīng)的報(bào)道多涉及其抗癌活性物質(zhì),而煙管菌活性多糖降血糖作用則較少報(bào)道。本研究以本實(shí)驗(yàn)室分離的多孔菌科煙管菌為實(shí)驗(yàn)材料,對(duì)其種屬鑒定、活性多糖提取及降血糖作用進(jìn)行研究,主要結(jié)果如下:1.通過對(duì)真菌進(jìn)行ITS測(cè)序,經(jīng)BLAST比對(duì)鑒定菌種為擔(dān)子菌綱非褶菌目多孔菌科煙管菌屬煙管菌。2.以菌絲干重和多糖含量為標(biāo)準(zhǔn),其中以多糖含量為主,篩選出的最適合的液體發(fā)酵培養(yǎng)基組分為:乳糖20g,酵母浸粉3.5g,MgS04.7H2O1g,KH2P042g,去皮馬鈴薯 100 g,H20 1 L。3.以菌絲干重為標(biāo)準(zhǔn),篩選出的最適的發(fā)酵條件為:pH 6,培養(yǎng)時(shí)間8 d,培養(yǎng)溫度 27℃轉(zhuǎn)速160r·min-1 接種量 8mL,裝液量 100mL/250mL。4.為了探索超聲波輔助提取煙管菌菌絲體多糖的最佳工藝,在單因素試驗(yàn)的基礎(chǔ)上選取水料比、超聲時(shí)間、超聲溫度三個(gè)因素為影響因子,利用響應(yīng)面Box-Behnken中心組合試驗(yàn)的范圍和水平,以煙管菌菌絲體多糖提取率為響應(yīng)值,進(jìn)一步優(yōu)化了超聲波輔助提取多糖的條件。通過研究和探討三個(gè)因素的最佳水平范圍和相互之間的作用,建立的影響菌絲體多糖提取率的模型為Y = 8.56-0.20X1-0.25X2 + 0.27X3 + 0.080X1X2 + 0.010X1X3-0.025X2X3-0.66X12-0.54X22-0.42X32。由統(tǒng)計(jì)學(xué)分析結(jié)果可以知道,方程中一次項(xiàng)X1、X2、X3,二次項(xiàng)X12、X22、X23對(duì)多糖提取率有非常顯著的影響(p0.01),并且交互項(xiàng)X1X2對(duì)多糖提取率有顯著(p0.05)的影響。根據(jù)回歸分析結(jié)果得到的超聲波輔助提取煙管菌菌絲體多糖的最佳工藝條件為:水料比48:1,超聲時(shí)間27 min,超聲溫度53℃。在此條件下菌絲體多糖提取率達(dá)到8.57%。5.將煙管菌多糖配制成不同濃度水溶液,測(cè)定各自的DPPH清除率,在濃度為1.5 mg·mL-1時(shí),DPPH清除率可以達(dá)到73.39%。6.用IBMX+DEX+Insulin聯(lián)合的方法將3T3-L1前脂肪細(xì)胞成功誘導(dǎo)為脂肪細(xì)胞,選擇3μmol/LDEX對(duì)進(jìn)行脂肪細(xì)胞進(jìn)行誘導(dǎo),建立了胰島素抵抗(IR)細(xì)胞模型,經(jīng)過研究發(fā)現(xiàn)煙管菌菌絲體多糖具有降糖活性。7.為了對(duì)多糖分子形貌進(jìn)行表征,分別對(duì)多糖進(jìn)行拉曼光譜和掃描電鏡。采用532 nm激光光源,測(cè)試了煙管菌多糖的拉曼光譜。光譜中出現(xiàn)了 440 cm-1峰表明多糖中含有葡萄糖。而通過對(duì)多糖進(jìn)行掃描電鏡,在不同倍數(shù)物鏡下的煙孔菌多糖呈現(xiàn)不同形態(tài)。200 μm物鏡下可以看到多糖為枯葉狀,表面粗糙;在50 μm物鏡下則呈不規(guī)則的碎石狀,而在5 μm物鏡下多糖主要呈規(guī)則的蠶豆、小球形態(tài),表面平滑。
[Abstract]:There are many reports about the biological effects of tobacco tube bacteria on the biological effects of the bacteria, and the antitumor activity of the bacteria is less reported. The results are as follows: 1. by ITS sequencing of fungi and BLAST comparison, the strain was identified as the strain.2. of the genus basidiomycetes of the genus polyporia. The dry weight of mycelium and the content of polysaccharide were the standard, and the most suitable liquid fermentation culture medium was divided into lactose 20g, yeast extract 3.5g, MgS04.7H2O1. G, KH2P042g, peeled potato 100 g, H20 1 L.3. were the standard of mycelial dry weight. The optimum fermentation conditions were: pH 6, incubation time 8 D, 160r. Min-1 inoculation at 27 centigrade temperature, 8mL, and liquid volume 100mL/250mL.4. in order to explore the optimum process of ultrasonic assisted extraction of mycelium polysaccharides from the mycelium of tobacco tube bacteria, in the basis of single factor test On the basis of the selection of water material ratio, ultrasonic time and ultrasonic temperature three factors as the influencing factors, using the range and level of the response surface Box-Behnken center combination test, the conditions of ultrasonic assisted extraction of polysaccharide were further optimized with the response value of the polysaccharide extraction rate of mycelium of the mycelium of the tobacco tube bacteria. The optimum level range of three factors was studied and discussed. The interaction between Y = 8.56-0.20X1-0.25X2 + 0.27X3 + 0.080X1X2 + 0.010X1X3-0.025X2X3-0.66X12-0.54X22-0.42X32. was established by the model of influencing the polysaccharide extraction rate of mycelium. The one term X1, X2, X3, two term X12, X22, and X23 had a very significant effect on the extraction rate of polysaccharide (P0.01). And the interaction term X1X2 has a significant effect on the extraction rate of polysaccharide (P0.05). The optimum technological conditions for ultrasonic assisted extraction of Polysaccharide from the mycelium of the mycelia obtained by the regression analysis are as follows: the ratio of water to 48:1, the ultrasonic time 27 min, and the ultrasonic temperature of 53. Under this condition, the polysaccharide extraction rate of mycelium is 8.57%.5. and the polysaccharide of the tobacco tube bacteria is prepared. The DPPH scavenging rate of different concentrations of water was measured. When the concentration was 1.5 mg. ML-1, the DPPH clearance rate could be achieved by 73.39%.6. using IBMX+DEX+Insulin to induce the 3T3-L1 preadipocytes to be successfully induced as adipocytes, and 3 u mol/LDEX were selected to induce the adipocytes, and the insulin resistance (IR) cell model was established. It was found that the polysaccharides of the mycelium of the mycelium were characterized by the hypoglycemic activity of.7. in order to characterize the molecular morphology of polysaccharides. The Raman and scanning electron microscopy of polysaccharide were carried out respectively. The Raman spectra of the polysaccharide were measured with 532 nm laser light source. The peak of 440 cm-1 in the spectrum showed that the glucose was contained in the polysaccharide. Under the microscope, the polysaccharides under different multiple objective lenses showed different forms of.200 mu m objective. The polysaccharides could be seen as dry leaves and rough surface. Under the 50 mu m objective lens, the irregular macadam was found. Under the 5 m objective lens, the polysaccharide mainly showed the regular broad bean, the shape of the ball and the surface was smooth.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284;R285

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 ;鍋爐煙管濕式洗管機(jī)防塵設(shè)備介紹[J];鐵道勞動(dòng)衛(wèi)生通訊;1976年02期

2 高雙芳;;丹東機(jī)務(wù)段煙管除銹作業(yè)衛(wèi)生學(xué)評(píng)價(jià)[J];鐵道勞動(dòng)衛(wèi)生通訊;1978年03期

3 鄭淑鵬;;手工制卷煙中的毒物含量[J];國(guó)外醫(yī)學(xué)(衛(wèi)生學(xué)分冊(cè));1992年06期

4 吳康祖;燃?xì)忮仩t煙管裂紋延伸導(dǎo)致管板裂紋原因分析[J];鐵道勞動(dòng)安全衛(wèi)生與環(huán)保;2004年02期

相關(guān)會(huì)議論文 前1條

1 房業(yè)清;安文廣;劉志輝;;關(guān)于DZ系列熱水鍋爐前管板孔橋開裂和煙管前端磨損原因及解決辦法[A];安全責(zé)任 重在落實(shí)——第四屆吉林安全生產(chǎn)論壇論文集[C];2011年

相關(guān)重要報(bào)紙文章 前3條

1 張瑩;安裝不當(dāng)可致煙機(jī)隱患 方太業(yè)內(nèi)首推預(yù)埋煙管[N];科技日?qǐng)?bào);2009年

2 記者 趙士振　通訊員 李竹;勝利河口廠“電動(dòng)煙管清理器”獲國(guó)家專利[N];中國(guó)石化報(bào);2010年

3 劉文;國(guó)外吸煙禮俗[N];廠長(zhǎng)經(jīng)理日?qǐng)?bào);2001年

相關(guān)碩士學(xué)位論文 前1條

1 陳蕾;煙管菌多糖的分離制備及降血糖活性研究[D];沈陽(yáng)農(nóng)業(yè)大學(xué);2017年

，

本文編號(hào)：1952887