本文選題：通便湯 + 慢傳輸便秘�。� 參考：《南京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:檢測通便湯對水通道蛋白3(AQP3)、水通道蛋白4(AQP4)的影響,明確通便湯對AQP3、AQP4的調(diào)節(jié)作用,并探討通便湯治療慢傳輸便秘的作用機(jī)理。研究方法:將70只大鼠隨機(jī)分為正常組(20只)與模型組(50只),正常組進(jìn)食普通飼料,模型組進(jìn)食含有復(fù)方地芬諾酯的飼料。模型復(fù)制成功后,處死正常組、模型組大鼠各10只,檢測腸道推進(jìn)程度及AQP3、AQP4表達(dá)及分布。剩余的10只正常組大鼠仍為正常組,剩余的40只模型組大鼠分為空白對照組、通便湯組、福松組、莫沙必利組,分別給予相應(yīng)干預(yù)。運(yùn)用炭墨推進(jìn)法檢測腸道傳輸功能,計(jì)數(shù)大鼠結(jié)腸內(nèi)存留大便,檢測結(jié)腸壁AQP3、AQP4的表達(dá)及在粘膜的分布及相對含量。結(jié)果:1模型復(fù)制成功后,與正常組比,模型組炭墨推進(jìn)率低(P0.01)、存留大便多(P0.01);AQP3MOD值模型組高于正常組(p0.01);AQP4MOD值模型組高于正常組(p0.05)。2大鼠用藥后,大便粒數(shù)及含水量:與空白對照組比,其余4組均比較高(p0.05);通便湯組與莫沙必利組差異不顯著(p0.05),但高于福松組(p0.05)。炭墨推進(jìn)率:與空白對照組比,通便湯組、莫沙必利組較高(p0.01),福松組差異不明顯(p0.05)。結(jié)腸存留大便:通便湯組、福松組、莫沙必利組均與空白對照組有顯著差異(p0.001)。3 IHC:AQP3主要分布在結(jié)腸粘膜頂部絨毛上皮細(xì)胞上,杯狀細(xì)胞表達(dá)較少。著色主要是在細(xì)胞膜頂部、基底及腔面,其中頂部表達(dá)最多,空白對照組著色較深�？瞻讓φ战M含量顯著高于其余4組,其次為福松組,其含量高于通便湯組(p0.05)。AQP4主要在吸收細(xì)胞上表達(dá),且以局灶表達(dá)為主,而在杯狀細(xì)胞中無表達(dá),黏膜頂部的表達(dá)高于底部,細(xì)胞基底面、腔面的表達(dá)高于側(cè)壁。通便湯組與莫沙必利組無差異(p0.05),其余每兩組之間均有顯著差異(p0.01)。4Western-blot:空白對照組的AQP3、AQP4含量明顯高于正常組(p0.001),通便湯組、福松組比空白對照組低(p0.05),莫沙必利組含量明顯低于空白對照組(p0.01)。5熒光定量-pcr:AQP3:與空白對照組比,福松組與之接近(p0.05),莫沙必利組次之(p0.05),通便湯組最低(p0.01)。AQP4:與空白對照組比,通便湯組較低(p0.05),福松組、莫沙必利組均無顯著差異(p0.05)。結(jié)論:1通便湯可明顯改善便秘癥狀,通過下調(diào)AQP3、AQP4調(diào)節(jié)腸內(nèi)水分吸收,治療慢傳輸便秘效果良好。2本實(shí)驗(yàn)中,治療慢傳輸便秘效果,通便湯最優(yōu),莫沙必利與之相近,福松次之。3通便湯與莫沙必利作用機(jī)制相似,但安全性更高,故臨床上治療慢傳輸便秘可能會(huì)更勝一籌。
[Abstract]:Objective: to investigate the effect of Tongbian decoction on aquaporin 3 aquaporin (AQP3) and aquaporin 4 (AQP4), to clarify the regulating effect of Tongbian decoction on AQP3 and AQP4, and to explore the mechanism of Tongbian decoction in the treatment of slow transit constipation. Methods: 70 rats were randomly divided into normal group (n = 20) and model group (n = 50). After the model was successfully copied, 10 rats in normal group and 10 rats in model group were killed. The intestinal propulsion degree and the expression and distribution of AQP3 AQP4 were detected. The remaining 10 normal rats were still normal, and the remaining 40 model groups were divided into blank control group, Tongbian decoction group, Fusong group and mosapride group, respectively. The intestinal transport function was detected by carbon ink propulsive method, and the colon defecation was counted. The expression of AQP3AAQP4 in colon wall and the distribution and relative content of AQP3AAQP4 in mucous membrane were detected. Results after the successful replication of the 1 / 1 model, compared with the normal group, the propelling rate of carbon ink in the model group was lower than that in the normal group (P 0.01), and the AQP3MOD value in the model group was higher than that in the normal control group (P 0.01) and AQP4MOD value in the model group was higher than that in the normal group (p 0.05.2), the number of feces and the water content in the model group were higher than those in the blank control group. There was no significant difference between Tongbian decoction group and mosapride group (P 0.05), but it was higher than that in Fusong group (P 0.05). The propelling rate of carbon and ink: compared with the blank control group, Tongbian decoction group and mosapride group were higher than that of the control group (P 0.01), but the difference of Fusong group was not significant (p 0.05). Colonic defecation: Tongbian decoction group, Fusapride group and mosapride group were significantly different from the blank control group in the distribution of p0.001t.3 IHC:AQP3 in the epithelial cells of the top of the colonic mucosa, and the expression of goblet cells was less. The staining was mainly on the top of the cell membrane, the basement and the surface of the cavity, the top of which was the most expressed, and the blank control group was deeply stained. The content of the blank control group was significantly higher than that of the other four groups, followed by the Fusong group, and its content was higher than that of the Tongbian decoction group, which was mainly expressed on the absorption cells and mainly on the focal cells, but not in the goblet cells, and the expression at the top of the mucous membrane was higher than that at the bottom. The expression of cell basal surface and cavity surface was higher than that of lateral wall. There was no difference between Tongbian decoction group and mosapride group, but there was significant difference between the other two groups. The content of AQP3 and AQP4 in the blank control group was significantly higher than that in the normal control group (P 0.001), and that in the Tongbian decoction group was significantly higher than that in the control group. Compared with the blank control group, the Fosong group was lower than the blank control group (P < 0.05), and the content of mosapride group was significantly lower than that of the blank control group (P 0.01). The fluorescence quantitative -pcr1: AQP3: compared with the blank control group, the Fusong group was close to the control group, the mosapride group was the second group, the lowest p0.01 .AQP4: compared with the blank control group. There was no significant difference between the Tongbian decoction group and the Fusapride group (P 0.05). Conclusion the constipation symptom can be obviously improved by using the decoction: through down-regulating AQP3AAQP4 to regulate the absorption of water in the intestine, the effect of treating slow transit constipation is good in this experiment, the effect of Tongbian decoction is the best, and mosapride is similar to the constipation. The mechanism of Fusong decoction is similar to that of mosapride, but the safety is higher, so the clinical treatment of slow transit constipation may be better.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 顏帥;錢海華;陳映輝;樂音子;孫明明;王曉鵬;;基于治療便秘方劑的頻次分析探討便秘從氣津論治理論[J];吉林中醫(yī)藥;2016年02期

2 王曉玲;李汀;江梅;;水通道蛋白9在便秘型腸易激綜合征患者結(jié)腸黏膜表達(dá)的研究[J];胃腸病學(xué)和肝病學(xué)雜志;2015年09期

3 錢海華;徐天舒;曾莉;顏帥;張榮枝;;通便顆粒調(diào)節(jié)慢傳輸型便秘大鼠結(jié)腸水通道蛋白3,水通道蛋白8表達(dá)的研究[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2014年24期

4 占煜;陳泰宇;唐詩宇;劉芳;孔鵬飛;覃勤;唐學(xué)貴;;從水通道蛋白3介導(dǎo)的結(jié)腸水液代謝思考治療慢性便秘的藥物研究[J];川北醫(yī)學(xué)院學(xué)報(bào);2014年06期

5 丁超;顧紅;陳玉根;楊柏霖;朱秉宜;陳紅錦;;“養(yǎng)陰潤腸方”用藥分析及其治療慢傳輸型便秘藥理學(xué)探究[J];遼寧中醫(yī)雜志;2014年11期

6 辛玉;張紅星;周利;;功能性便秘大鼠模型的研究進(jìn)展[J];湖北中醫(yī)雜志;2014年03期

7 向雪蓮;侯曉華;;《2013年中國慢性便秘診治指南》重點(diǎn)解讀[J];中國實(shí)用外科雜志;2013年11期

8 顏帥;劉佃溫;曾莉;錢海華;;張東岳從臟論治便秘經(jīng)驗(yàn)[J];中國中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志;2013年10期

9 顏帥;曾莉;錢海華;;養(yǎng)陰潤腸湯治療慢傳輸型便秘54例[J];南京中醫(yī)藥大學(xué)學(xué)報(bào);2013年04期

10 王品;張有成;;慢傳輸型便秘的病因及發(fā)病機(jī)制研究進(jìn)展[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2012年30期

，

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