本文選題：白虎加人參湯 + 糖尿病��； 參考：《黑龍江中醫(yī)藥大學》2017年碩士論文

【摘要】：目的:研究白虎加人參湯對糖尿病大鼠模型血清中丙二醛(MDA)及背根神經(jīng)節(jié)(Drg)中TRPA1mRNA表達水平的影響,探討白虎加人參湯對糖尿病及其并發(fā)癥的療效及其作用機制,為臨床應(yīng)用提供實驗依據(jù)。方法:清潔級健康雄性SD大鼠45只,體重220±10g,普通飼料適應(yīng)性喂養(yǎng)1周,隨機選取10只作為空白對照組,剩余35只禁食12小時后給予單次腹腔注射鏈脲佐菌素(STZ)50mg/kg,72小時后尾靜脈采血,共31只大鼠血糖≥16.7mmol/L,糖尿病大鼠模型造模成功,4只血糖未達標,造模不成功,成膜后大鼠隨機分為白虎加人參湯組10只、α-硫辛酸組10只、模型對照組11只。白虎加人參湯組給予9.38g/kg/d灌胃,α-硫辛酸組給予20mg/kg/d腹腔注射,空白對照組、模型對照組給予同體積生理鹽水灌胃,8周后測定相關(guān)指標,ELISA法測定大鼠血糖、血清MDA含量;光鏡觀察大鼠坐骨神經(jīng)病理形態(tài)學改變;RT-PCR檢測大鼠背根神經(jīng)節(jié)中TRPA1mRNA的表達水平。結(jié)果:1、大鼠體重:治療前各組大鼠體重基本相同,無統(tǒng)計學意義(P0.05);治療后白虎加人參湯組、α-硫辛酸組大鼠體重較空白對照組體重增長緩慢,具有統(tǒng)計學意義(P0.01),較模型對照組體重增長快,具有統(tǒng)計學意義(P0.05),白虎加人參湯組和α-硫辛酸組比較,無統(tǒng)計學意義(P0.05)。2、大鼠血糖:造模72h后,與空白對照組比較,模型對照組、白虎加人參湯組和α-硫辛酸組大鼠血糖均升高,具有統(tǒng)計學意義(P0.01)。治療后,白虎加人參湯組和α-硫辛酸組大鼠血糖較模型組下降,具有統(tǒng)計學意義(P0.01),白虎加人參湯組和α-硫辛酸組比較,白虎加人參湯組大鼠血糖下降較多,具有統(tǒng)計學意義(P0.05)。3、大鼠血清MDA:與空白對照組比較,模型對照組、白虎加人參湯組和α-硫辛酸組大鼠血清MDA水平升高,具有統(tǒng)計學意義(P0.05);與模型對照組比較,白虎加人參湯組和α-硫辛酸組大鼠血清MDA水平均下降,具有統(tǒng)計學差異(P0.01);白虎加人參湯組和α-硫辛酸組比較,無統(tǒng)計學意義(P0.05)。4、大鼠坐骨神經(jīng)形態(tài)學光鏡觀察:空白對照組大鼠坐骨神經(jīng)纖維排列整齊,結(jié)構(gòu)清楚,神經(jīng)束模連續(xù)完整,厚度基本一致,細胞核清晰,軸索、髓鞘結(jié)構(gòu)完整;模型對照組神經(jīng)纖維排列紊亂,分布不均,神經(jīng)束模斷裂破損,厚度不均勻,郎飛氏結(jié)間隙增寬,細胞核固縮,軸索變性,腫脹或皺縮,髓鞘呈節(jié)段性脫失;白虎加人參湯組和α-硫辛酸組神經(jīng)纖維排列較整齊,束模部分斷裂,部分細胞核固縮,部分軸索腫脹或皺縮,髓鞘個別脫失,程度均較模型對照組輕。5、大鼠背根神經(jīng)節(jié)細胞TRPA1mRNA表達水平:與空白對照組比較,模型對照組、白虎加人參湯組和α-硫辛酸組大鼠背根神經(jīng)節(jié)細胞中TRPA1mRNA的表達升高,具有統(tǒng)計學意義(P0.01);與模型對照組比較,白虎加人參湯組、硫辛酸組大鼠背根神經(jīng)節(jié)細胞TRPA1mRNA的表達均下降,具有統(tǒng)計學意義(P0.05);白虎加人參湯組和α-硫辛酸組比較,白虎加人參湯組下降更明顯,具有統(tǒng)計學差異(P0.05)。結(jié)論:1、白虎加人參湯可以降低糖尿病大鼠血糖。2、白虎加人參湯可以降低糖尿病大鼠血清MDA的含量減輕氧化應(yīng)激狀態(tài)。3、白虎加人參湯可以改善糖尿病大鼠坐骨神經(jīng)病理形態(tài)。4、白虎加人參湯可以下調(diào)糖尿病大鼠背根神經(jīng)節(jié)中TRPA1mRNA的表達。
[Abstract]:Objective: To study the effect of white tiger and human ginseng soup on the level of TRPA1mRNA expression of malondialdehyde (MDA) and dorsal root ganglion (Drg) in the serum of diabetic rats, and to explore the effect and mechanism of the white tiger plus human Ginseng Decoction on diabetes and its complications and to provide experimental basis for clinical application. Methods: 45 healthy male SD rats with a clean grade and 220 body weight were used. For 1 weeks, 10 rats were randomly selected as blank control group for 1 weeks. The remaining 35 rats were given a single intraperitoneal injection of streptozotocin (STZ) 50mg/kg after 12 hours of fasting, and after 72 hours the tail vein was collected. A total of 31 rats had blood sugar above 16.7mmol/L. The model of diabetic rats was successfully built, 4 blood glucose failed to reach the standard, and the model was unsuccessful. After the formation of the model, the membrane formation was unsuccessful and membrane formation after forming the membrane. Rats were randomly divided into 10 rats in the white tiger plus Shen Shen Decoction group, 10 in the alpha lipoic acid group and 11 in the model control group. The white tiger plus human ginseng decoction group was given 9.38g/kg/d gavage, the alpha lipoic acid group was given 20mg/kg/d intraperitoneal injection and the control group was given the control group. The model control group was given the same volume of normal saline, and the related indexes were measured after 8 weeks. The blood glucose of rats was measured by ELISA method and the serum M was measured. DA content; the pathological changes of the sciatic nerve in rats were observed by light microscope, and the expression level of TRPA1mRNA in the dorsal root ganglion of rats was detected by RT-PCR. Results: 1, the weight of rats in the rats before treatment was basically the same, without statistical significance (P0.05); the weight of the rats in the group of alpha lipoic acid group after treatment was higher than that of the blank control group. Slow, statistically significant (P0.01), compared with the model control group, the body weight increased rapidly and had statistical significance (P0.05). The white tiger plus Shen Shen Decoction group and the alpha lipoic acid group had no statistical significance (P0.05).2, the rat blood sugar was compared with the blank control group, the model control group, the white tiger addition Shen Shen Decoction group and the alpha lipoic acid group increased the blood sugar level. Higher, with statistical significance (P0.01). After treatment, the blood glucose of the White Tiger Group and the alpha lipoic acid group decreased compared with the model group, with statistical significance (P0.01). Compared with the white tiger and the alpha lipoic acid group and the alpha lipoic acid group, the blood glucose decreased more in the white tiger and the human ginseng decoction group, with statistical significance (P0.05).3, the rat serum MDA: and the blank control group Compared with the model control group, the serum MDA level of the rats in the white tiger plus human ginseng soup group and the alpha lipoic acid group increased, with statistical significance (P0.05). Compared with the model control group, the serum MDA level of the white tiger and the alpha lipoic acid group decreased with statistical difference (P0.01), and the white tiger plus human ginseng decoction group and the alpha lipoic acid group were not statistically significant. Study significance (P0.05).4, rat sciatic nerve morphological light microscope observation: the blank control group the sciatic nerve fibers arranged neatly, the structure is clear, the nerve bundle model is continuous and complete, the thickness is basically the same, the nucleus clear, the axon, the myelin sheath structure complete; the model control group the nerve fiber arrangement is disorderly, the distribution is uneven, the nerve bundle mode fracture breakage, thickness not Even, the clearance of the Lang flying junction widened, nucleus retraction, axonal degeneration, swelling or shrinkage, the myelin sheath showed segmental loss; the white tiger and the alpha lipoic acid group and the group of alpha lipoic acid group were neatly arranged, part of the nucleus was fractured, some nuclei were retracted, some axons swelled or crinkled, and the myelin sheath was individually lost, and the degree was less.5 than the model control group. The expression level of TRPA1mRNA in root ganglion cells: compared with the blank control group, the expression of TRPA1mRNA in the dorsal root ganglion cells of the model control group, the white tiger adding Shen Shen Decoction group and the alpha lipoic acid group increased, with statistical significance (P0.01). Compared with the model control group, the white tiger plus ginseng decoction group and the rat dorsal root ganglion cells of the lipoic acid group were TRPA1mRNA. The expression of the white tiger plus human ginseng decoction group and the group of alpha lipoic acid, white tiger and human ginseng soup group decreased more obviously, with statistical difference (P0.05). Conclusion: 1, white tiger plus Shen Shen decoction can reduce the blood sugar.2 of diabetic rats, white tiger plus ginseng decoction can reduce the content of serum MDA in diabetic rats and reduce oxidation. Stress state.3, white tiger plus Shen Shen decoction can improve the pathological morphological.4 of sciatic nerve in diabetic rats, and white tiger plus Shen Shen decoction can reduce the expression of TRPA1mRNA in the dorsal root ganglion of diabetic rats.

【學位授予單位】：黑龍江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R285.5;R-332

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