本文選題：脂肪顆粒 + 凍存液�。� 參考：《大連醫(yī)科大學(xué)》2017年碩士論文

【摘要】：研究背景:由于自體脂肪組織的生物相容性優(yōu)于人工生物制品及異體材料,且無免疫排斥現(xiàn)象,成本相對低廉,目前已成為理想的軟組織填充材料,得到了廣泛臨床應(yīng)用。然而移植脂肪高吸收率、低存活率的問題一直困擾著整形外科醫(yī)師,若想達(dá)到長期滿意效果,常需進(jìn)行二次手術(shù)移植。國內(nèi)外學(xué)者一直在試圖尋找更好的方法以提高脂肪移植成活率、盡量減少吸脂手術(shù)次數(shù):如一次吸脂后將移植后多余的脂肪顆粒體外凍存,二次移植時(shí)復(fù)溫后再注射;在移植組織中添加PRP、SVF等不同成分;選擇合適的純化方式及抽吸管徑、負(fù)壓等等。各類研究都在進(jìn)行,關(guān)于吸脂尚無一套統(tǒng)一的操作規(guī)范,本研究選擇脂肪組織移植供區(qū)、脂肪組織凍存液配比兩個(gè)因素對脂肪組織活性的影響進(jìn)行實(shí)驗(yàn)研究。目的:探索不同配比凍存液對不同部位凍存脂肪組織活性的影響,以優(yōu)化脂肪組織凍存液比例并為臨床脂肪移植供區(qū)選擇提供理論依據(jù)。方法:1.常規(guī)收集45例吸脂患者大腿內(nèi)、外側(cè)及腹部的顆粒脂肪。將每例標(biāo)本平均分為兩份分別存入A、B兩種比例的凍存液中,于 80°C的深低溫冰箱中凍存6個(gè)月。其中A組凍存液配比為:60%小牛血清、15%二甲基亞砜(DMSO)、25%高糖培養(yǎng)基(DMEM)。B組為:30%小牛血清、15%二甲基亞砜、55%高糖培養(yǎng)基。2.6個(gè)月后將標(biāo)本復(fù)蘇,用HE染色觀察組織形態(tài),計(jì)算完整細(xì)胞比率;臺(tái)盼藍(lán)染色計(jì)算死亡細(xì)胞比例;肌酸激酶實(shí)驗(yàn)測定標(biāo)本的細(xì)胞破壞率;免疫組化CD105檢測脂肪干細(xì)胞形態(tài)及數(shù)量。3.不同凍存液儲(chǔ)存的脂肪顆粒復(fù)溫后,每個(gè)注射點(diǎn)取0.2ml顆粒脂肪移植于裸鼠背部皮下,術(shù)后4周處死裸鼠,取出移植脂肪組織,進(jìn)行稱重、測量體積、HE染色,透射電鏡觀察細(xì)胞形態(tài)。結(jié)果:HE染色:大腿內(nèi)側(cè)A組脂肪細(xì)胞完整率為78.3±2.03%,B組為66.4±3.23%;大腿外側(cè)A組脂肪細(xì)胞完整率為70.5±4.53%,B組為55.4±6.03%;腹部A組脂肪細(xì)胞完整率為65.1±4.63%,B組為51.9±5.08%。每個(gè)部位的A、B兩組之間差異有統(tǒng)計(jì)學(xué)意義(P0.05),每組凍存液中大腿內(nèi)側(cè)與其余兩個(gè)部位間差異有統(tǒng)計(jì)學(xué)意義。臺(tái)盼藍(lán)染色示A組凍存液條件下的脂肪細(xì)胞拒染率較B組高,同一組內(nèi)大腿內(nèi)側(cè)脂肪細(xì)胞拒染率較高,肌酸激酶結(jié)果與臺(tái)盼藍(lán)一致,以A組(60%小牛血清、15%DMSO、25%DMEM)長期凍存的脂肪活性較高。來源于大腿外側(cè)、大腿內(nèi)側(cè)及腹部的脂肪標(biāo)本中,其中大腿內(nèi)側(cè)的活性較高,有利于脂肪移植的成活。結(jié)論:A組凍存液長期凍存脂肪活性較高。大腿內(nèi)側(cè)顆粒脂肪活性高于大腿外側(cè)及腹部。
[Abstract]:Background: since the biocompatibility of autogenous adipose tissue is superior to that of artificial biological products and allogeneic materials and there is no immune rejection and the cost is relatively low, autologous adipose tissue has become an ideal soft tissue filling material and has been widely used in clinical practice. However, the problems of high fat absorption rate and low survival rate have been a problem for plastic surgeons. If they want to achieve long-term satisfactory results, it is often necessary to carry out secondary surgery transplantation. Scholars at home and abroad have been trying to find a better way to improve the survival rate of fat transplantation, as far as possible to reduce the number of liposuction operations: such as after the first liposuction transplantation of excess fat particles frozen in vitro, secondary transplantation after rewarming and then injected; Different components such as PRPN SVF were added to the transplanted tissue, and the proper purification methods and suction diameter, negative pressure were selected. All kinds of studies are being carried out, and there is no uniform operation standard for liposuction. In this study, the effects of two factors, adipose tissue transplantation donor area and ratio of frozen fat tissue storage solution, on adipose tissue activity were studied experimentally. Objective: to explore the effect of different proportion of frozen liquid on the activity of frozen adipose tissue in different parts in order to optimize the proportion of frozen liquid of adipose tissue and to provide theoretical basis for the selection of donor area for clinical fat transplantation. Method 1: 1. Granule fat in the inner, lateral and abdominal thighs of 45 patients with liposuction was routinely collected. Each specimen was divided into two parts in two proportions of Agna B, and frozen in 80 擄C deep cryopreservation refrigerator for 6 months. The ratio of cryopreservation solution in group A was: 1. 60% calf serum, 15% DMSOxone, 25% high sugar culture medium, DMEMN. Group B: 30% calf serum, 15% dimethyl sulfoxide, 55% high sugar. After 2.6 months, the specimens were resuscitated, the tissue morphology was observed by HE staining, and the complete cell ratio was calculated. Trypan blue staining was used to calculate the proportion of dead cells, creatine kinase assay was used to determine the cell destruction rate, and immunohistochemical CD105 was used to detect the morphology and number of adipose stem cells. After rewarming the fat particles stored in different frozen solution, the fat particles of 0.2ml were taken from each injection point and transplanted subcutaneously into the back of nude mice. After 4 weeks of operation, the nude mice were killed, the adipose tissue was removed and weighed, and the volume of fat was measured by HE staining. Cell morphology was observed by transmission electron microscope. Results the complete rate of adipocytes in group A was 78.3 鹵2.03and 66.4 鹵3.23in group B, 70.5 鹵4.53 in group B, 55.4 鹵6.03in group B, and 65.1 鹵4.63in group B, 51.9 鹵5.08in group B.Results the complete rate of adipocytes in group A was 78.3 鹵2.03g, and that in group B was 61.9 鹵5.08.The percentage of adipocytes in group B was 70.5 鹵4.53 and 55.4 鹵6.03in group B, respectively. There was significant difference between two groups (P 0.05), and there was significant difference between the inner thigh and the other two parts in the frozen liquid of each group. Trypan blue staining showed that the rejection rate of adipocytes in group A was higher than that in group B, and the rejection rate of adipocytes in the inner thigh of the same group was higher than that in group B, and the results of creatine kinase were consistent with those of trypan blue. The fat activity of group A (60% calf serum) was higher than that of group A (DMEM). Among the fat samples from lateral thigh, medial thigh and abdomen, the activity of medial thigh is higher, which is beneficial to the survival of fat transplantation. Conclusion the fat activity of group A was higher than that of group A. The fat activity of medial thigh granules was higher than that of lateral thigh and abdomen.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R622

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 鄭志芳;張益;李筱s，

本文編號(hào)：1856790