本文選題：太赫茲時域光譜技術(shù) + 太赫茲成像　； 參考：《中央民族大學》2017年碩士論文

【摘要】：太赫茲時域光譜技術(shù)(THz-TDS)是20世紀80年代以來新發(fā)展起來的一種技術(shù),利用光電導的方法產(chǎn)生THz輻射脈沖,具有帶寬大、信噪比高、能夠在室溫中工作等優(yōu)點。THz脈沖透射樣品或者在樣品表面發(fā)生反射,測量THz電場強度與時間的變化關(guān)系,對測得的數(shù)據(jù)進行經(jīng)傅里葉變換后得到頻域上幅值與相位的變化關(guān)系,從而獲得樣品的信息。太赫茲技術(shù)憑借其獨特的優(yōu)勢在中草藥的質(zhì)量檢測、定性定量和光譜成像分析中得到廣泛的應用。本文主要利用THz-TDS技術(shù)和光譜成像技術(shù)在中草藥光譜檢測及腫瘤診斷方面做了如下幾個部分的工作:首先,利用太赫茲時域光譜技術(shù)對不同含水量的天麻及不同鉻含量的明膠樣品分別進行了測量,得到其THz光譜,分別計算獲得天麻脫水過程中與頻率相關(guān)的吸收系數(shù)曲線,及不同鉻含量明膠與頻率相關(guān)的吸收系數(shù)曲線。發(fā)現(xiàn)含水量與吸收系數(shù)、明膠中鉻含量與吸收系數(shù)都呈線性正相關(guān)的關(guān)系,從而根據(jù)測量的結(jié)果對樣品的質(zhì)量進行評估。其次,利用太赫茲時域光譜技術(shù)重新檢測了青蒿素及其衍生物(雙氫青蒿素、青蒿琥酯、蒿甲醚)等四種青蒿素類藥物在太赫茲波段的吸收特性,分別得到它們在特定波段(0.2-2.7THz)的吸收光譜。并對實驗結(jié)果與現(xiàn)有的指紋譜數(shù)據(jù)進行了對比分析。結(jié)果顯示,絕大部分的共振峰與文獻[24]中的一致,但峰位和強度略有差異,并且有新的峰位出現(xiàn)。然后,討論了樣品制備和數(shù)據(jù)處理方法對數(shù)據(jù)結(jié)果的影響,以及分子結(jié)構(gòu)與峰位的關(guān)系。并基于此進一步確認了數(shù)據(jù)的有效性。結(jié)果為進一步研究青蒿素及其衍生物的藥性提供參考,并對其無損鑒別、定量定性分析提供了一種快速高效的鑒定方法。最后,運用太赫茲時域光譜成像技術(shù)對肝臟正常組織與肝臟腫瘤組織、腎臟正常組織與腎臟腫瘤組織分別進行探測,分別得到了他們的太赫茲時域光譜圖,經(jīng)數(shù)據(jù)處理得到了腫瘤組織與正常組織的吸收系數(shù)光譜圖,并對正常組織與腫瘤組織的吸收光譜分別進行了分析。然后利用逐點掃描太赫茲成像系統(tǒng)分別對肝臟、腎臟的腫瘤組織進行了成像,分別得到它們在時域顯示模式和頻域顯示模式下的THz重構(gòu)圖,成像結(jié)果能較好區(qū)分出腫瘤組織與正常組織。最后,對成像結(jié)果進行了討論,提出了實驗過程中出現(xiàn)的問題,并針對已有的問題提供了解決的辦法,為以后太赫茲技術(shù)在腫瘤診斷領(lǐng)域的研究奠定了基礎(chǔ)。
[Abstract]:Terahertz time-domain spectroscopy (THz-TDS) is a new technology developed since 1980s. It uses photoconductive method to generate THz radiation pulse, which has a large bandwidth and high signal-to-noise ratio (SNR). It can work at room temperature and other advantages. THz pulse transmission samples or on the surface of the sample reflect, measure the change of THz electric field intensity and time, and get the change of amplitude and phase in frequency domain by Fourier transform. In order to obtain the sample information. Terahertz technology has been widely used in the quality detection, qualitative and quantitative analysis and spectral imaging analysis of Chinese herbal medicine with its unique advantages. In this paper, THz-TDS and spectral imaging techniques are used in the detection of Chinese herbal medicine spectrum and tumor diagnosis as follows: first, The THz spectra of Gastrodia elata and gelatin samples with different water content and different chromium content were measured by terahertz time-domain spectroscopy. The absorption coefficient curves related to frequency in the dehydration process of Gastrodia elata were calculated, respectively. And the absorption coefficient curve of different chromium content gelatin and frequency correlation. It was found that there was a linear positive correlation between water content and absorption coefficient, chromium content in gelatin and absorption coefficient, so the quality of the sample was evaluated according to the measured results. Secondly, the absorption characteristics of artemisinin and its derivatives (dihydroartemisinin, artesunate, artemisinin) in terahertz band were re-examined by THz time-domain spectroscopy. Their absorption spectra of 0.2-2.7 THZ were obtained respectively. The experimental results are compared with the existing fingerprint data. The results show that most of the resonance peaks are consistent with those in literature [24], but the peak position and intensity are slightly different, and new peaks appear. Then, the influence of sample preparation and data processing on the data results and the relationship between molecular structure and peak position are discussed. Based on this, the validity of the data is further confirmed. The results provided a rapid and efficient method for further study of artemisinin and its derivatives, nondestructive identification and quantitative qualitative analysis of artemisinin and its derivatives. Finally, terahertz time-domain imaging technique was used to detect normal liver tissue and liver tumor tissue, kidney normal tissue and renal tumor tissue, and their terahertz time-domain spectra were obtained. The absorption coefficient spectra of tumor and normal tissues were obtained by data processing, and the absorption spectra of normal and normal tissues were analyzed respectively. Then the liver and kidney tumor tissues were examined by point-by-point scanning terahertz imaging system, and their THz reconstructed images in time-domain and frequency-domain display modes were obtained, respectively. The imaging results can distinguish tumor tissue from normal tissue. Finally, the imaging results are discussed, the problems in the experiment are put forward, and the solutions to the existing problems are provided, which will lay a foundation for the future research of terahertz technology in the field of tumor diagnosis.
【學位授予單位】：中央民族大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R284;R730.4;O657.3

【參考文獻】

相關(guān)期刊論文 前10條

1 湯祖華;;原子吸收分光光度法測定明膠空心膠囊中鉻含量不確定度的評定[J];海峽藥學;2015年08期

2 葛建華;徐繼華;;電感耦合等離子體質(zhì)譜法測定明膠空心膠囊中鉻元素含量[J];中國藥業(yè);2014年21期

3 劉尚建;余菲;李凱;周靜;;太赫茲光譜與成像在生物醫(yī)學領(lǐng)域中的應用[J];物理;2013年11期

4 齊娜;張卓勇;相玉紅;;太赫茲技術(shù)在醫(yī)學檢測和診斷中的應用研究[J];光譜學與光譜分析;2013年08期

5 YANG YuPing;LEI XiangYun;YUE Ai;ZHANG ZhenWei;;Temperature-dependent THz vibrational spectra of clenbuterol hydrochloride[J];Science China(Physics,Mechanics & Astronomy);2013年04期

6 楊玉平;董睿林;張振偉;;六種生物藥物分子的高分辨太赫茲光譜研究[J];光譜學與光譜分析;2012年11期

7 何明霞;郭帥;;太赫茲波技術(shù)在藥學上的應用研究[J];電子測量與儀器學報;2012年08期

8 史會齊;白雁;謝彩俠;龔海燕;王星;;近紅外光譜法快速測定六味地黃丸中水分含量[J];實驗室研究與探索;2011年05期

9 尚麗平;鄧琥;劉娟;夏祖學;廖小春;;基于光導開關(guān)脈沖偏置電壓的太赫茲時域光譜系統(tǒng)[J];光電工程;2011年03期

10 汪一帆;尉萬聰;周鳳娟;薛照輝;;太赫茲(THz)光譜在生物大分子研究中的應用[J];生物化學與生物物理進展;2010年05期

，

本文編號：1814254