本文選題：脊髓損傷 + 音猬因子��； 參考：《江蘇大學(xué)》2017年碩士論文

【摘要】：目的:探究應(yīng)用可緩釋音猬因子(sonic hedgehog,SHH)的纖維蛋白凝膠(fibrin glue,FG)支架促大鼠脊髓完全橫斷損傷的修復(fù)效果。方法:(1)制作體外緩釋SHH的纖維蛋白膠支架模型,探究緩釋效果。(2)選用健康的SD大鼠60只,制作大鼠脊髓完全橫斷模型,隨機(jī)分成三組:僅做脊髓橫斷損傷組(SCI),只植入纖維蛋白膠組(FG),植入音猬因子-支架移植組(F-SHH)。術(shù)后每周記錄大鼠雙下肢功能活動(BBB評分)。術(shù)后12周取出脊髓組織,采用免疫組織化學(xué)染色和免疫蛋白印跡檢測,觀察脊髓損傷的近遠(yuǎn)端神經(jīng)元存活情況及神經(jīng)絲蛋白(neurofilament200,NF200),生長再生相關(guān)蛋白(growth associated protein-43,GAP43),膠質(zhì)纖維酸性蛋白(glial fibrillary acidic protein,GFAP)表達(dá)情況。結(jié)果:(1)包封SHH的纖維蛋白凝膠擁有良好的緩釋SHH的效果。(2)F-SHH組BBB評分從術(shù)后到第十二周較FG、SCI組上升明顯(P0.05)。(3)免疫組化結(jié)果顯示:F-SHH組脊髓橫斷端有一些神經(jīng)元存活且排列規(guī)則,許多神經(jīng)纖維未完全潰變。SCI組細(xì)胞凋亡明顯,纖維潰變嚴(yán)重,退變空泡大,FG組較SCI組稍好。(4)Western Blot結(jié)果顯示:F-SHH組NF200,GAP43相對含量高于FG、SCI組(P0.05),而GFAP相對含量低于FG、SCI組(P0.05)。結(jié)論:SHH緩釋纖維蛋白支架緩釋效果良好,對大鼠脊髓完全橫斷損傷的修復(fù)有明顯的促進(jìn)作用,SHH對于脊髓損傷的修復(fù)機(jī)理值得進(jìn)一步研究。
[Abstract]:Aim: to investigate the effect of fibrin gel fibrin FG (fibrin gluein FG) scaffold on the repair of spinal cord complete transection injury (sci) in rats with sustainable-release hedgehog factor (SHH).Methods the fibrin gel scaffold model of sustained release of SHH in vitro was made by using: 1) 60 healthy Sprague-Dawley rats were selected to establish the spinal cord transection model.They were randomly divided into three groups: the spinal cord transection group (SCI), the fibrin glue group (FGG), and the Hedgehog factor-stent graft group (F-SHHH).The BBB scores were recorded weekly after operation.Spinal cord tissues were taken out 12 weeks after operation and were detected by immunohistochemistry and Western blot.The survival of proximal and distal neurons in spinal cord injury and the expression of neurofilament protein neurofilament200, growth and regeneration related protein associated protein-43 and glial fibrillary acidic protein were observed.Results the fibrin gel encapsulated with SHH had a good effect of sustained release SHH. The BBB score of F-SHH group was significantly higher than that of FGG sci group from postoperative to 12th week. The immunohistochemical results showed that there were some neurons in the transverse end of spinal cord in the FGF-SHH group.The results of Western Blot showed that the relative content of NF200 GAP43 in FG group was higher than that in FGSCI group, while the relative content of GFAP in FG group was lower than that in FGSCI group.Conclusion the sustained release effect of% SHH fibrin scaffold is good and can promote the repair of spinal cord complete transection injury in rats. The mechanism of SHH on spinal cord injury should be further studied.
【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R651.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 程志堅;祝文;李浩鵬;賀西京;;巨噬細(xì)胞促進(jìn)神經(jīng)干細(xì)胞向小鼠脊髓損傷區(qū)遷移[J];細(xì)胞與分子免疫學(xué)雜志;2016年09期

2 Wen-Chen Ji;Xiao-Wei Zhang;Yu-Sheng Qiu;;Selected suitable seed cell, scaffold and growth factor could maximize the repair effect using tissue engineering method in spinal cord injury[J];World Journal of Experimental Medicine;2016年03期

3 班德翔;劉洋;曹泰瑋;馮世慶;;組織工程學(xué)治療脊髓損傷的研究進(jìn)展[J];中華骨科雜志;2016年16期

4 陳紅麗;徐佳迪;南文濱;郭雙;李武山;連杰;豐慧根;林俊堂;;膠原/纖維蛋白膠復(fù)合藥膜的性質(zhì)及其體外抑瘤效應(yīng)初步研究[J];國際生物醫(yī)學(xué)工程雜志;2016年01期

5 彭展;陳亮;顧勇;張志明;孟斌;周峰;楊惠林;唐天駟;;胸腰段爆裂骨折伴不完全脊髓損傷的手術(shù)療效分析[J];中華創(chuàng)傷雜志;2015年11期

6 李棋;李箭;;堿性成纖維細(xì)胞生長因子及纖維蛋白膠在骨科的運(yùn)用[J];中國組織工程研究;2015年12期

7 邱滿樂;連小峰;徐建廣;;生物支架用于脊髓損傷治療研究進(jìn)展[J];國際骨科學(xué)雜志;2014年05期

8 周繼輝;趙叢然;田飛鵬;邵林;何曉峰;李強(qiáng);崔斌;;脊髓納米組織工程支架研究進(jìn)展[J];國際生物醫(yī)學(xué)工程雜志;2013年02期

9 徐向東;盧向紅;施勇;葉冠雄;朱錫元;;纖維蛋白膠順鉑凝膠化療的緩釋特性[J];中華醫(yī)學(xué)雜志;2011年18期

10 于鵬;宋展昭;孫培峰;陳君;鞠萍;隋艷;劉聘婷;;納米支架材料在脊髓損傷修復(fù)中的應(yīng)用[J];中國組織工程研究與臨床康復(fù);2010年47期

相關(guān)博士學(xué)位論文 前1條

1 邵志成;來源于大鼠脊髓星形膠質(zhì)細(xì)胞的神經(jīng)干細(xì)胞誘導(dǎo)分化成運(yùn)動神經(jīng)元的研究[D];上海交通大學(xué);2011年

，

本文編號：1772057