本文選題：根尖乳頭干細(xì)胞 + 腫瘤壞死因子-α�。� 參考：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究腫瘤壞死因子-α對鼠根尖乳頭干細(xì)胞增殖及多向分化能力的影響,初步探討腫瘤壞死因子-α對根尖乳頭干細(xì)胞生物學(xué)特性的影響。方法:將細(xì)胞分為實(shí)驗(yàn)組(腫瘤壞死因子-α濃度為5、10、15、20、30、40、50ng/mL)和對照組(腫瘤壞死因子-α濃度為0ng/mL),使用CCK-8法檢測細(xì)胞的增殖能力;采用堿性磷酸酶活性、茜素紅染色及實(shí)時(shí)定量PCR檢測細(xì)胞成骨/成牙本質(zhì)能力;油紅O染色檢測細(xì)胞成脂能力;實(shí)時(shí)定量PCR檢測血管相關(guān)基因的表達(dá)。結(jié)果:CCK-8顯示:各濃度組均能促進(jìn)根尖乳頭干細(xì)胞增殖(P0.05),其中10ng/m L腫瘤壞死因子-α的促進(jìn)作用最為顯著;堿性磷酸酶活性顯示:各濃度組均能明顯降低根尖乳頭干細(xì)胞的堿性磷酸酶活性(P0.05);茜素紅染色顯示:隨著腫瘤壞死因子-α濃度的增加,礦化結(jié)節(jié)逐漸變小,形成數(shù)量也逐漸變少;實(shí)時(shí)定量PCR顯示:3天,實(shí)驗(yàn)組骨鈣蛋白、牙本質(zhì)涎磷蛋白、牙本質(zhì)基質(zhì)蛋白-1表達(dá)量降低,其中骨鈣蛋白、牙本質(zhì)基質(zhì)蛋白-1差異具有統(tǒng)計(jì)學(xué)意義(P0.05),骨涎蛋白表達(dá)量增加(P0.05)。7天時(shí),骨鈣蛋白、牙本質(zhì)涎磷蛋白、牙本質(zhì)基質(zhì)蛋白-1表達(dá)量降低,其中牙本質(zhì)基質(zhì)蛋白-1差異具有統(tǒng)計(jì)學(xué)意義(P0.05),骨涎蛋白表達(dá)量與對照組相比仍稍有增加(P0.05);14天時(shí),骨涎蛋白、骨鈣蛋白、牙本質(zhì)基質(zhì)蛋白-1表達(dá)量均明顯降低(P0.05),牙本質(zhì)涎磷蛋白表達(dá)量稍有增加(P0.05)。油紅O染色結(jié)果顯示:實(shí)驗(yàn)組隨著腫瘤壞死因子-α濃度的增加,脂滴形成數(shù)量逐漸減少;實(shí)時(shí)定量PCR顯示:3、7天時(shí),血管生成素1、血管內(nèi)皮生長因子A、血小板內(nèi)皮細(xì)胞黏附分子-1表達(dá)量降低(P0.05)。結(jié)論:腫瘤壞死因子-α對根尖乳頭干細(xì)胞的增殖有明顯促進(jìn)作用,且抑制根尖乳頭干細(xì)胞的成骨/成牙本質(zhì)、成脂能力及血管相關(guān)基因的表達(dá)。
[Abstract]:Aim: to study the effects of tumor necrosis factor- 偽 (TNF- 偽) on the proliferation and multidirectional differentiation of rat apical papilla stem cells (RSCs), and to explore the effects of TNF- 偽 on the biological characteristics of root-tip papillary stem cells (RSCs).Methods: the cells were divided into two groups: the experimental group (tumor necrosis factor- 偽 concentration was 5n10151530ng / mL) and the control group (the tumor necrosis factor- 偽 concentration was 0ng / mLL). The proliferative ability of the cells was measured by CCK-8 assay, the activity of alkaline phosphatase was measured by alkaline phosphatase assay, the activity of alkaline phosphatase was measured by alkaline phosphatase assay.Alizarin red staining and real-time quantitative PCR were used to detect osteoblast / dentin ability; oil red O staining was used to detect the adipogenic ability of cells; and real-time quantitative PCR was used to detect the expression of vascular related genes.Results the results showed that every concentration group could promote the proliferation of apical papilla stem cells (P0.05), especially 10ng/m L tumor necrosis factor- 偽 (TNF- 偽).Alkaline phosphatase activity showed that the alkaline phosphatase activity of apical papilla stem cells was significantly decreased in each concentration group, and alizarin red staining showed that the mineralized nodules gradually decreased with the increase of tumor necrosis factor- 偽 concentration.Real time quantitative PCR showed that the expression of osteocalcin, dentin sialophosphorus protein and dentin matrix protein -1 decreased in the experimental group on day 3, in which osteocalcin, osteocalcin, and dentin matrix protein 1 were expressed in the experimental group.The difference of dentin matrix protein 1 was statistically significant (P 0.05). The expression of osteocalcin, dentin sialophosphorus protein and dentin matrix protein 1 decreased after the increase of bone sialoprotein expression.The difference of dentin matrix protein -1 was statistically significant (P 0.05). The expression of bone sialoprotein was still slightly increased after 14 days compared with the control group.The expression of dentine matrix protein-1 was significantly decreased, while that of dentin sialophosphate protein was slightly increased.The results of oil red O staining showed that the number of lipid droplets gradually decreased with the increase of tumor necrosis factor- 偽 concentration in the experimental group, and the real-time quantitative PCR showed that at 7 days after the tumor necrosis factor- 偽 concentration,The expression of angiopoietin 1, vascular endothelial growth factor A and platelet endothelial cell adhesion molecule-1 decreased (P 0.05).Conclusion: tumor necrosis factor- 偽 can significantly promote the proliferation of apical papilla stem cells, and inhibit osteogenesis / dentin formation, fat-forming ability and expression of vascular related genes of apical papilla stem cells.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R781

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